2012
DOI: 10.1152/physiolgenomics.00060.2012
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Mitochondrial proteomic analysis reveals deficiencies in oxygen utilization in medullary thick ascending limb of Henle in the Dahl salt-sensitive rat

Abstract: In the present studies we isolated mitochondria from mTAL of SS and salt-resistant control strain SS.13 BN rats on 0.4 and 8% salt diet for 7 days and performed a proteomic analysis. Purity of mTAL and mitochondria isolations exceeded 93.6 and 55%, respectively. Using LC/MS spectral analysis techniques we identified 96 mitochondrial proteins in four biological mTAL mitochondria samples, run in duplicate, as defined by proteins with a false discovery rate Ͻ5% and scan count Ն2. Seven of these 96 proteins, inclu… Show more

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Cited by 44 publications
(48 citation statements)
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“…Medullary interstitial infusion of a fumarate precursor in the SS.13 BN rats significantly increased medullary tissue H 2 O 2 and exacerbated salt-induced hypertension. Consistent with these observations, a proteomic analysis of isolated mTAL mitochondria found nine proteins to be reduced in SS rats compared with SS.13 BN rats (203). The downregulation of these proteins was predicted to reduce the efficiency of mitochondrial O 2 utilization, which was confirmed by high-resolution respirometry of the mTAL cells and in mitochondria isolated from the outer medulla of SS rats.…”
Section: Medullary O 2 ·ϫ No and H 2 O 2 As Determinants Of Blood mentioning
confidence: 52%
“…Medullary interstitial infusion of a fumarate precursor in the SS.13 BN rats significantly increased medullary tissue H 2 O 2 and exacerbated salt-induced hypertension. Consistent with these observations, a proteomic analysis of isolated mTAL mitochondria found nine proteins to be reduced in SS rats compared with SS.13 BN rats (203). The downregulation of these proteins was predicted to reduce the efficiency of mitochondrial O 2 utilization, which was confirmed by high-resolution respirometry of the mTAL cells and in mitochondria isolated from the outer medulla of SS rats.…”
Section: Medullary O 2 ·ϫ No and H 2 O 2 As Determinants Of Blood mentioning
confidence: 52%
“…28 Chromosome 13 contains several critical genes such as genes encoding renin and NADPH subunit p67 phox and several studies utilizing this model illustrate its critical role in BP regulation. [28][29][30][31][32][33] After 3 weeks on a HS diet, MAP was significantly lower in SS. 13 BN rats compared with SS rats, as Figure 1.…”
Section: Enac Plays a Role In Development Of Salt-sensitive Hypertensmentioning
confidence: 96%
“…Downregulation of proteins connected with energy metabolism predicted reduction of oxygen utilization in mitochondria in medullary thick ascending limb of Henle's loop of salt sensitive rats 94 . 86 .…”
Section: Hypertensionmentioning
confidence: 95%
“…Using salt-sensitive rats as model organism for hypertension, effect of salt-induced hypertension on mitochondria of thick ascending limb of Henle's loop was tested by proteomics 94 . Downregulation of proteins connected with energy metabolism predicted reduction of oxygen utilization in mitochondria in medullary thick ascending limb of Henle's loop of salt sensitive rats 94 .…”
Section: Hypertensionmentioning
confidence: 99%
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