Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration

Valdez, Laura B.; Bombicino, Silvina Sonia; Iglesias, Darío E.; A, Ivana Rukavina Mikusic; Boveris, Alberto

doi:10.15406/ijmboa.2018.03.00051

Cited by 9 publications

(6 citation statements)

References 67 publications

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“…Lastly, we treated SWIR-HD with a panel of biologically relevant reactive oxygen and nitrogen species, as well as redox active metal ions (Figure 2c). Although the parent HD is prone to rapid oxidative decomposition, 43,44 SWIR-HD was remarkably resistant against strong oxidants such as peroxynitrite even at levels 100,000-fold higher than the reported cellular concentration of 5-10 nM. 44 Likewise, we observed no significant loss of SWIR-HD in the presence of redox-active metals (Fe and Cu) capable of generating ROS via Fenton and Fenton-like chemistry, respectively.…”

Section: In Vitro Characterization and Stability Assaysmentioning

confidence: 48%

Synthesis of silicon-substituted hemicyanines for multimodal SWIR imaging

East

Lee

Smaga³

et al. 2022

Preprint

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SWIR dyes offer many advantages over their more common NIR congeners; however, the available options are limited. New SWIR imaging agents can be accessed by remodeling existing NIR molecules (i.e., hemicyanines (HD)). In this study, we syn-thesized SWIR-HD, a modified HD featuring silicon-dimethyl and benzo[cd]indolium groups that are designed to red-shift the absorbance and emission to 988 and 1126 nm, respectively. SWIR-HD was tested in three cell lines and shown to exhibit ex-cellent biocompatibility.

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Section: In Vitro Characterization and Stability Assaysmentioning

confidence: 48%

Synthesis of silicon-substituted hemicyanines for multimodal SWIR imaging

East

Lee

Smaga³

et al. 2022

Preprint

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“…Similar to ROS, these reactive nitrogen species (RNS) are highly reactive and can oxidize thiols and nitrate proteins ( Adams et al, 2015 ). Since RNS generation is directly dependent on superoxide ( Valdez et al, 2018 ), focus will be placed on ROS.…”

Section: Ros/rns: Double-edged Swordsmentioning

confidence: 99%

Iron and Cadmium Entry Into Renal Mitochondria: Physiological and Toxicological Implications

Thévenod

Lee

Garrick

2020

Front. Cell Dev. Biol.

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Regulation of body fluid homeostasis is a major renal function, occurring largely through epithelial solute transport in various nephron segments driven by Na + /K + -ATPase activity. Energy demands are greatest in the proximal tubule and thick ascending limb where mitochondrial ATP production occurs through oxidative phosphorylation. Mitochondria contain 20–80% of the cell’s iron, copper, and manganese that are imported for their redox properties, primarily for electron transport. Redox reactions, however, also lead to reactive, toxic compounds, hence careful control of redox-active metal import into mitochondria is necessary. Current dogma claims the outer mitochondrial membrane (OMM) is freely permeable to metal ions, while the inner mitochondrial membrane (IMM) is selectively permeable. Yet we recently showed iron and manganese import at the OMM involves divalent metal transporter 1 (DMT1), an H + -coupled metal ion transporter. Thus, iron import is not only regulated by IMM mitoferrins, but also depends on the OMM to intermembrane space H + gradient. We discuss how these mitochondrial transport processes contribute to renal injury in systemic (e.g., hemochromatosis) and local (e.g., hemoglobinuria) iron overload. Furthermore, the environmental toxicant cadmium selectively damages kidney mitochondria by “ionic mimicry” utilizing iron and calcium transporters, such as OMM DMT1 or IMM calcium uniporter, and by disrupting the electron transport chain. Consequently, unraveling mitochondrial metal ion transport may help develop new strategies to prevent kidney injury induced by metals.

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“…SWIR-HD was remarkably resistant against all analytes tested. For instance, peroxynitrite is a strong oxidant that is notorious for dye decomposition; however, SWIR-HD was stable even against levels 100,000-fold higher than reported cellular concentrations (5–10 nm) …”

Section: Design Of Silicon-substituted Hemicyanine Dyes (Sihds)mentioning

confidence: 99%

Synthesis of Silicon-Substituted Hemicyanines for Multimodal SWIR Imaging

et al. 2022

View full text Add to dashboard Cite

SWIR dyes offer many advantages over their more common NIR congeners; however, the available options are limited. New SWIR imaging agents can be accessed by remodeling existing NIR molecules (i.e., hemicyanines (HDs)). In this study, we synthesized SWIR-HD, a modified HD featuring dimethylsilicon and benzo[cd]indolium groups that are designed to red-shift the absorbance and emission to 988 and 1126 nm, respectively. SWIR-HD was employed to visualize the liver and tumors via multimodal SWIR imaging.

show abstract

Mitochondrial peroxynitrite generation is mainly driven by superoxide steady-state concentration rather than by nitric oxide steady-state concentration

Cited by 9 publications

References 67 publications

Synthesis of silicon-substituted hemicyanines for multimodal SWIR imaging

Synthesis of silicon-substituted hemicyanines for multimodal SWIR imaging

Iron and Cadmium Entry Into Renal Mitochondria: Physiological and Toxicological Implications

Synthesis of Silicon-Substituted Hemicyanines for Multimodal SWIR Imaging

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