Mitochondrial membrane tension governs fission

Mahecic, Dora; Carlini, Lina; Kleele, Tatjana; Colom, Adai; Goujon, Antoine; Matile, Stefan; Roux, Aurélien; Manley, Suliana

doi:10.1016/j.celrep.2021.108947

Cited by 53 publications

(51 citation statements)

References 79 publications

(99 reference statements)

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“…The time resolution of the slow and EDA modes was evaluated by calculating the minimal width of constriction measured during the experiment. The measurement was performed similar to the method described in (11). Parameters are the ones used for the mitochondria analysis with Caulobacter values in brackets.…”

Section: Discussionmentioning

confidence: 99%

“…Note 2). This data set was recorded on a fast dual-color SIM setup at Janelia Farm (19) and published previously (11). The ground truth was computed from the intensity in the Drp1 channel, multiplied by the negative product of principal curvatures of the mitochondrial signal (Sup.…”

Section: Sim Imaging and Training Datamentioning

confidence: 99%

“…Training data was acquired at the fast SIM using a SLM and LCD for generating and controlling the illumination pattern (19). The training data consisted of constriction events published previously (11).…”

Section: Sim Imaging and Training Datamentioning

confidence: 99%

“…Such events are challenging to capture, because they are infrequent and can occur at any location within the mitochondrial network distributed throughout the cell. Once initiated, constriction can proceed to division within tens of seconds (11). To detect events of interest, we trained a neural network with a U-net architecture (12) to recognise mitochondrial constrictions in the presence of dynamin related protein 1 (DRP1).…”

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confidence: 99%

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Event-driven acquisition for content-enriched microscopy

Mahecic

Stepp

Zhang

et al. 2021

Preprint

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In fluorescence microscopy, the amount of information that can be collected from the sample is limited, often due to constraints imposed by photobleaching and phototoxicity. Here, we report an event-driven acquisition (EDA) framework, which combines real-time, neural network-based recognition of events of interest with automated control of the imaging parameters in an instant structured illumination microscope (iSIM). On-the-fly prioritization of imaging rate or experiment duration is achieved by switching between a slow imaging rate to detect the onset of biological events of interest and a fast imaging rate to enable high information content during their progression. In this way, EDA allows the data capture of mitochondrial and bacterial divisions at imaging rates that match their dynamic timescales, while extending the accessible imaging duration, and thereby increases the density of relevant information in the acquired data.

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Section: Discussionmentioning

confidence: 99%

Section: Sim Imaging and Training Datamentioning

confidence: 99%

Section: Sim Imaging and Training Datamentioning

confidence: 99%

mentioning

confidence: 99%

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Event-driven acquisition for content-enriched microscopy

Mahecic

Stepp

Zhang

et al. 2021

Preprint

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“…Currently, most of the proteins known to be associated with mitochondrial fission are GTPase family proteins, including Drp1, Fis1, Mff, and Mid49/51 (Chen K. H. et al, 2018;Kamerkar et al, 2018;Yu et al, 2019a,b). Also present are dynamin protein 2 (Dyn2/Dnm2), and Bax interacting factor 1 (Bif1), which assist Drp1 in regulating mitochondrial fission (Mahecic et al, 2021), and ganglioside-induced differentiationassociated protein 1 (GDAP1) (Rzepnikowska and Kochanski, 2018), death-associated protein 3 (DAP3) (Xiao et al, 2015), and mitochondria protein 18 (MTP18) (Aung et al, 2019).…”

Section: Mitochondrial Fission-related Proteinsmentioning

confidence: 99%

Molecular Machinery and Pathophysiology of Mitochondrial Dynamics

Chiu

Kuo

2021

Front. Cell Dev. Biol.

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Mitochondria are double-membraned organelles that exhibit fluidity. They are the main site of cellular aerobic respiration, providing energy for cell proliferation, migration, and survival; hence, they are called “powerhouses.” Mitochondria play an important role in biological processes such as cell death, cell senescence, autophagy, lipid synthesis, calcium homeostasis, and iron balance. Fission and fusion are active processes that require many specialized proteins, including mechanical enzymes that physically alter mitochondrial membranes, and interface proteins that regulate the interaction of these mechanical proteins with organelles. This review discusses the molecular mechanisms of mitochondrial fusion, fission, and physiopathology, emphasizing the biological significance of mitochondrial morphology and dynamics. In particular, the regulatory mechanisms of mitochondria-related genes and proteins in animal cells are discussed, as well as research trends in mitochondrial dynamics, providing a theoretical reference for future mitochondrial research.

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Fluorescent Flippers: Small‐Molecule Probes to Image Membrane Tension in Living Systems

et al. 2023

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Flipper probes have been introduced as small molecule fluorophores to image physical forces, that is, membrane tension in living systems. Their emergence over one decade is described, from evolution in design and synthesis to spectroscopic properties. Responsiveness to physical compression in equilibrium at the ground state is identified as the ideal origin of mechanosensitivity to image membrane tension in living cells. A rich collection of flippers is described to deliver and release in any subcellular membrane of interest in a leaflet‐specific manner. Chalcogen‐bonding cascade switching and dynamic covalent flippers are developed for super‐resolution imaging and dual‐sensing of membrane compression and hydration. Availability and broad use in the community validate flipper probes as a fine example of the power of translational supramolecular chemistry, moving from fundamental principles to success on the market.

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Mitochondrial membrane tension governs fission

Cited by 53 publications

References 79 publications

Event-driven acquisition for content-enriched microscopy

Event-driven acquisition for content-enriched microscopy

Molecular Machinery and Pathophysiology of Mitochondrial Dynamics

Fluorescent Flippers: Small‐Molecule Probes to Image Membrane Tension in Living Systems

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