Mitochondrial Membrane Permeability After Nanosecond Electromagnetic Pulsed Exposure

Жаркова, Л. П.; Romanchenko, I. V.; Buldakov, Michael A.; Priputnev, Pavel V.; Bolshakov, M. A.; Rostov, V. V.

doi:10.1109/ishce.2018.8521236

Cited by 3 publications

(1 citation statement)

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“…It was also indicated that nanosecond pulses caused recruitment of intracellular Ca 2+ [58]. It was previously observed that mitochondria and other membranous cell organelles are "porated" during exposure to nanosecond pulses [22,[59][60][61]. The authors also observed that after applying nsPEF, calcium ions are released from internal calcium-stores such as ER and mitochondria or taken up from outside the cell through permeabilization.…”

Section: Discussionmentioning

confidence: 93%

The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells

Kulbacka

Rembiałkowska

Szewczyk

et al. 2021

Cancers

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(1) Background: Calcium electroporation (CaEP) is based on the application of electrical pulses to permeabilize cells (electroporation) and allow cytotoxic doses of calcium to enter the cell. (2) Methods: In this work, we have used doxorubicin-resistant (DX) and non-resistant models of human breast cancer (MCF-7/DX, MCF-7/WT) and colon cancer cells (LoVo, LoVo/DX), and investigated the susceptibility of the cells to extracellular Ca2+ and electric fields in the 20 ns–900 ns pulse duration range. (3) Results: We have observed that colon cancer cells were less susceptible to PEF than breast cancer cells. An extracellular Ca2+ (2 mM) with PEF was more disruptive for DX-resistant cells. The expression of glycoprotein P (MDR1, P-gp) as a drug resistance marker was detected by the immunofluorescent (CLSM) method and rhodamine-123 efflux as an MDR1 activity. MDR1 expression was not significantly modified by nanosecond electroporation in multidrug-resistant cells, but a combination with calcium ions significantly inhibited MDR1 activity and cell viability. (4) Conclusions: We believe that PEF with calcium ions can reduce drug resistance by inhibiting drug efflux activity. This phenomenon of MDR mechanism disruption seems promising in anticancer protocols.

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