Mitochondrial Membrane Complex That Contains Proteins Necessary for tRNA Import in Trypanosoma brucei

Seidman, David; Johnson, David; Gerbasi, Vincent R.; Golden, Daniel E.; Orlando, Ron; Hajduk, Stephen L.

doi:10.1074/jbc.m111.300186

Cited by 21 publications

(9 citation statements)

References 51 publications

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“…Table S6 summarises the prediction of these features in the 279 genes. Another pathway whose components may co-express with protein import components is mitochondrial tRNA import (Esseiva et al, 2004;Pino et al, 2010;Sharma and Sharma, 2015), as in some organisms these two pathways are linked (Tschopp et al, 2011;Seidman et al, 2012). Mitochondrial tRNA import is likely essential for mitochondrial translation, for which components are enriched in the 43-gene dataset, proposing these 43 genes as candidate for component of this pathway.…”

Section: Identification Of New Mitochondrial Proteins Using An In Silmentioning

confidence: 99%

Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation

Lacombe

Maclean

Ovciarikova

et al. 2019

Molecular Microbiology

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Summary Apicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including fundamental processes such as mitochondrial translation. Despite evidence that mitochondrial translation is essential for parasite survival, it is largely understudied. Progress has been restricted by the absence of functional assays to detect apicomplexan mitochondrial translation, a lack of knowledge of proteins involved in the process and the inability to identify and detect mitoribosomes. We report the localization of 12 new mitochondrial proteins, including 6 putative mitoribosomal proteins. We demonstrate the integration of three mitoribosomal proteins in macromolecular complexes, and provide evidence suggesting these are apicomplexan mitoribosomal subunits, detected here for the first time. Finally, a new analytical pipeline detected defects in mitochondrial translation upon depletion of the small subunit protein 35 (TgmS35), while other mitochondrial functions remain unaffected. Our work lays a foundation for the study of apicomplexan mitochondrial translation.

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Section: Identification Of New Mitochondrial Proteins Using An In Silmentioning

confidence: 99%

Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation

Lacombe

Maclean

Ovciarikova

et al. 2019

Molecular Microbiology

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“…Thus, we expect that the machinery for mitochondrial tRNA import also is encoded by POMPs that encode core essential functions. It is even possible, as has been suggested for the mitochondrial IM (57,58), that some protein import factors might also be required for tRNA import. Interestingly, of the three newly discovered factors that are required in order to maintain normal mitochondrial morphology (Fig.…”

Section: Verification Of Om Localization Of Seven Pomps-despitementioning

confidence: 99%

Mitochondrial Outer Membrane Proteome of Trypanosoma brucei Reveals Novel Factors Required to Maintain Mitochondrial Morphology

Niemann

Wiese

Mani

et al. 2013

Molecular & Cellular Proteomics

141

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Trypanosoma brucei is a unicellular parasite that causes devastating diseases in humans and animals. It diverged from most other eukaryotes very early in evolution and, as a consequence, has an unusual mitochondrial biology. Moreover, mitochondrial functions and morphology are highly regulated throughout the life cycle of the parasite. The outer mitochondrial membrane defines the boundary of the organelle. Its properties are therefore key for understanding how the cytosol and mitochondria communicate and how the organelle is integrated into the metabolism of the whole cell. We have purified the mitochondrial outer membrane of T. brucei and characterized its proteome using label-free quantitative mass spectrometry for protein abundance profiling in combination with statistical analysis. Our results show that the trypanosomal outer membrane proteome consists of 82 proteins, twothirds of which have never been associated with mitochondria before.

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“…Most mitochondrial mRNAs are subjected to editing by a process first discovered in these cells [4] (reviewed in [5]). The mitochondrial genome of trypanosomes does not contain tRNAs and the whole set of these tRNAs needs to be imported by a mechanism that share components with the protein import machinery [6, 7]. Some respiratory complexes are incomplete or absent in some trypanosomatids, such as in Phytomonas spp.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial calcium transport in trypanosomes

Docampo

Vercesi

Huang

2014

Molecular and Biochemical Parasitology

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The biochemical peculiarities of trypanosomes were fundamental for the recent molecular identification of the long-sought channel involved in mitochondrial Ca2+ uptake, the mitochondrial Ca2+ uniporter or MCU. This discovery led to the finding of numerous regulators of the channel, which form a high molecular weight complex with MCU. Some of these regulators have been bioinformatically identified in trypanosomes, which are the first eukaryotic organisms described for which MCU is essential. In trypanosomes MCU is important for buffering cytosolic Ca2+ changes and for activation of the bioenergetics of the cells. Future work on this pathway in trypanosomes promises further insight into the biology of these fascinating eukaryotes, as well as the potential for novel target discovery.

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Mitochondrial Membrane Complex That Contains Proteins Necessary for tRNA Import in Trypanosoma brucei

Cited by 21 publications

References 51 publications

Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation

Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation

Mitochondrial Outer Membrane Proteome of Trypanosoma brucei Reveals Novel Factors Required to Maintain Mitochondrial Morphology

Mitochondrial calcium transport in trypanosomes

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