Mitochondrial maturation during neuronal differentiation <i>in vivo</i> and <i>in vitro</i>

Cordeau-Lossouarn, Laurence; Vayssière, Jean-Luc; Larcher, Jean-Christophe; Gros, François; Croizat, Bernard

doi:10.1016/0248-4900(91)90051-n

Cited by 28 publications

(18 citation statements)

References 21 publications

(23 reference statements)

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“…Considering that 1 cm 3 of brain consumes about 2 lmoles O 2 /min, while 1 cm 3 of mitochondria consumes about 0.8 lmoles O 2 /min (calculated from Mela and Seitz [8]), it may be paradoxically concluded that even if brain was made of mitochondria, these would not be sufficient to account for the measured O 2 combustion. Intriguingly, a burst of mitochondrial protein synthesis was reported during development of differentiating neuronal cells [9], while the number of mitochondria remained almost unchanged. been proposed.…”

Section: Introductionmentioning

confidence: 98%

Hypothesis of an Energetic Function for Myelin

Morelli

Ravera

Panfoli

2011

Cell Biochem Biophys

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Nervous system is a great oxygen consumer, but the site of oxygen absorption has remained elusive. Four proteomic studies have shown that the respiratory complexes I to V may be expressed in isolated myelin. Myelin is an outgrowth of glial cells, surrounding many axons in multiple spires both in peripheral and central nervous system. Recent quantitative analyses strongly support the daring hypothesis that myelin is functional in aerobic ATP production, to supply the neuron with chemical energy. A vision of myelin sheath as a structure devoted to the oxygen absorbance for glucose combustion in nervous system thank to its enormous surface, would be also supported by an impressive series of characteristics and properties of myelin that do not presently find an explanation, all of which are herein examined.

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Section: Introductionmentioning

confidence: 98%

Hypothesis of an Energetic Function for Myelin

Morelli

Ravera

Panfoli

2011

Cell Biochem Biophys

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“…Gels were incubated for 30 min in Amplify (Amersham) before drying and autoradiography with Amersham Hyperfilm. Proteins encoded by mitochondrial DNA were identified as described (13).…”

Section: Methodsmentioning

confidence: 99%

Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40.

Vayssière

Petit

Risler

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

285

150

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Rodent embryo cells immortalized with temperature-sensitive mutants of simian virus 40 large tumor (T) antigen have a proliferative potential that depends on temperature. At the restrictive temperature, heat-inactivation oflarge T antigen causes p53 release, growth arrest, and cell death.Morphological and molecular analysis indicate that the induced cell death corresponds to apoptosis. Flow cytometric analysis using a combination of forward light scatter and side scatter allows a discrimination of cells committed to apoptosis within the whole population. These cells display a reduction in cell size and a higher cellular density, confirming the apoptotic nature of the cell death. When cells exhibiting the morphological features of apoptosis were stained with a fluorescent probe of the mitochondrial membrane potential, a decreased accumulation of the dye was recorded. Measures of cellular respiration, performed with whole-cell populations, showed that the lower mitochondrial membrane potential (At,) correlates, as expected, with an uncoupling of electron transport from ATP production and is linked to the induction of apoptosis. We also show that this decrease in A'I is associated with a decrease in the rate ofmitochondrial translation. These events are detected at early stages of the apoptotic process, when most of the cells are not irreversibly committed to death, suggesting that mitochondria could be a primary target during apoptosis.Mammalian cells grown in culture exhibit a finite life-span for proliferation. After a variable number of divisions they stop dividing, undergo a variety of changes, and finally die. Some oncogenes have the ability to confer an unlimited proliferative potential (immortality) to primary cells in culture. In the case of polyomavirus-induced immortalization of rodent embryo fibroblasts, it has been shown that the unlimited proliferative capacity is maintained by the large tumor (T) antigen (1)(2)(3)(4) MATERIALS AND METHODSCell Lines and Cell Culture. The REtsAF and RELPB cell lines were isolated at low cell density from a rat embryo fibroblast culture infected with SV40 (2). REtsAF was obtained by using a temperature-sensitive tsA58 mutant and is temperature sensitive for immortalization, whereas RELPB was obtained with wild-type SV40 and is immortal at both 330 and 39.50C. REtsAF-Revl was derived from REtsAF by selection for proliferation at 39.50C (8

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“…Thus, acute changes in mitochondrial membrane potential, mitochondriogenesis, and mitochondrial gene expression are associated with neuronal differentiation (Cordeau-Lossouam et al, 1991; Vayssiere et al, 1992). Moreover, a reversible block in neu-0 1996 WILEY-LISS, INC. ron differentiation was induced by inhibition of mitochondrial mRNAs' translation but not by the specific inhibition of ATP synthase activity.…”

mentioning

confidence: 98%

Changes in mitochondrial activity during avian myoblast differentiation: Influence of triiodothyronine or v-erbA expression

Rochard¹,

Cassar-Malek

Marchal

et al. 1996

J. Cell. Physiol.

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Numerous data suggest that mitochondrial activity is involved in the regulation of cell growth and differentiation. Therefore, we have studied the changes in mitochondrial activity in avian myoblast cultures (QM7 line) undergoing differentiation or in BrdU-treated, differentiation-deficient cells. As we have previously shown that triiodothyronine and v-erb A expression stimulate myogenic differentiation, we have also observed their influence upon mitochondrial activity. Comparison of control and BrdU-treated myoblasts indicated that precocious differentiation events were associated with a stimulation of citrate synthase and cytochrome oxidase activities. They also induced a transient decrease in mitochondrial membrane potential assessed by rhodamine 123 uptake. In control myoblasts, a general stimulation of mitochondrial activity was recorded at cell confluence, prior to terminal differentiation. These events did not occur in BrdU-treated myoblasts, thus indicating that they were tightly linked to myoblast commitment. Whereas no significant triiodothyronine influence could be detected upon mitochondrial activity, we observed that v-erb A expression significantly depresses the mitochondrial membrane potential in control myoblasts. This action was not observed in BrdU-treated myoblasts, thus suggesting that it involves an indirect pathway linked to differentiation. Moreover, the oncoprotein abrogated the decrease in E2-PDH subunit level observed at cell confluence. These data underline that changes in mitochondrial activity occurred prior to myoblast terminal differentiation and could be involved in the processes regulating myogenesis. In addition, they provide the first evidence that the v-erb A oncoprotein influences mitochondrial activity.

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Mitochondrial maturation during neuronal differentiation in vivo and in vitro

Cited by 28 publications

References 21 publications

Hypothesis of an Energetic Function for Myelin

Hypothesis of an Energetic Function for Myelin

Commitment to apoptosis is associated with changes in mitochondrial biogenesis and activity in cell lines conditionally immortalized with simian virus 40.

Changes in mitochondrial activity during avian myoblast differentiation: Influence of triiodothyronine or v-erbA expression

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