Mitochondrial iron transport and homeostasis in plants

Jain, Anshika; Connolly, Erin L.

doi:10.3389/fpls.2013.00348

Cited by 55 publications

(32 citation statements)

References 66 publications

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“…It has been already shown in mammals and yeast that Fe may exit the mitochondria in a few forms: as heme, as Fe‐S clusters, or as Fe 2+ ions. As plants cells may synthesize heme exclusively in plastids, and then export it to the cytosol and mitochondria (Van Lis et al ., ; Mochizuki et al ., ), it is assumed that heme exporters may be absent in plant cell mitochondria (Jain and Connolly, ). However, protein candidates for the mitochondrial exporters of Fe‐S clusters and Fe 2+ have been identified in both yeast and plant cells.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess

Migocka

Małas

Maciaszczyk-Dziubińska

et al. 2018

The Plant Journal

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The plant metal tolerance protein family (MTP) includes 12 members that have been classified into three phylogenetically different subgroups - Zn-cation diffusion facilitator (CDF), Fe/Zn-CDF and Mn-CDF - based on their putative metal specificity. To date, only members belonging to the Zn-CDF or Mn-CDF group have been characterized functionally. The plant Fe/Zn-CDF subgroup includes two proteins, MTP6 and MTP7, but their function and metal specificity have not been confirmed. In this study we showed that cucumber CsMTP7 is a highly specific mitochondrial Fe importer that is able to confer yeast tolerance to Fe excess through increased accumulation of Fe in the mitochondria. We also demonstrated that CsMTP7 contributes to the increased accumulation of Fe in the mitochondria of Arabidopsis thaliana protoplasts. The transcripts and mitochondrial levels of CsMTP7 and ferritin - the iron-storing protein - are significantly increased in cucumber roots in response to Fe excess. This finding suggests that CsMTP7 and ferritin work in concert to accumulate Fe in plant mitochondria. As genes that encode orthologous proteins have been identified in phylogenetically distant organisms, including Archaea, cyanobacteria, humans and plants, but not in yeast, we concluded that the MTP7-mediated mitochondrial Fe accumulation may be conserved in the species, and express mitochondrial ferritin for mitochondrial Fe storage.

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Section: Introductionmentioning

confidence: 99%

Retracted: Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess

Migocka

Małas

Maciaszczyk-Dziubińska

et al. 2018

The Plant Journal

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“…In the present study, the expression of the XM_004490603 . 2 gene, encoding a mitoferrin‐like (a mitochondrial Fe transporter) involved in storage of Fe in mitochondria (Jain and Connolly, ), and the XM_004495449 . 2 gene, encoding the Yellow Strip‐like 1 (YSL1) protein involved in transport of nicotianamine (NA)‐Fe complexes into cells (Waters et al ., ), was up‐regulated by 2.13‐ and 2.26‐fold, respectively, in Mm SWRI9‐Pd/ Mm SWRI9‐Ps but not in Mc CP‐31‐Pd/ Mc CP‐31‐Ps (Table ), which might suggest Pi deficiency‐induced Fe over‐accumulation in Mm SWRI9‐induced nodules.…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis of nodules of two Mesorhizobium–chickpea associations with differential symbiotic efficiency under phosphate deficiency

et al. 2017

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Phosphate (Pi) deficiency is known to be a major limitation for symbiotic nitrogen fixation (SNF), and hence legume crop productivity globally. However, very little information is available on the adaptive mechanisms, particularly in the important legume crop chickpea (Cicer arietinum L.), which enable nodules to respond to low-Pi availability. Thus, to elucidate these mechanisms in chickpea nodules at molecular level, we used an RNA sequencing approach to investigate transcriptomes of the nodules in Mesorhizobium mediterraneum SWRI9-(MmSWRI9)-chickpea and M. ciceri CP-31-(McCP-31)-chickpea associations under Pi-sufficient and Pi-deficient conditions, of which the McCP-31-chickpea association has a better SNF capacity than the MmSWRI9-chickpea association during Pi starvation. Our investigation revealed that more genes showed altered expression patterns in MmSWRI9-induced nodules than in McCP-31-induced nodules (540 vs. 225) under Pi deficiency, suggesting that the Pi-starvation-more-sensitive MmSWRI9-induced nodules required expression change in a larger number of genes to cope with low-Pi stress than the Pi-starvation-less-sensitive McCP-31-induced nodules. The functional classification of differentially expressed genes (DEGs) was examined to gain an understanding of how chickpea nodules respond to Pi starvation, caused by soil Pi deficiency. As a result, more DEGs involved in nodulation, detoxification, nutrient/ion transport, transcriptional factors, key metabolic pathways, Pi remobilization and signalling were found in Pi-starved MmSWRI9-induced nodules than in Pi-starved McCP-31-induced nodules. Our findings have enabled the identification of molecular processes that play important roles in the acclimation of nodules to Pi deficiency, ultimately leading to the development of Pi-efficient chickpea symbiotic associations suitable for Pi-deficient soils.

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“…The mitochondrial FRO3 was upregulated (Fig. 3A), suggesting that mitochondria continue to import Fe (Jain and Connolly, 2013). MIT1 and MIT2, homologs of the Mitochondrial Iron Transporter well-characterized in other species (Bashir et al, 2011) were not differentially expressed, but they generally do not respond to Fe deficiency (Balk and Schaedler, 2014).…”

Section: Iron-dependent Respiratory Complexes In the Mitochondria Arementioning

confidence: 92%

Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds

et al. 2018

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During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double mutant is unable to mobilize Fe stores and does not develop in the absence of external Fe. We used RNA sequencing to compare gene expression in and wild type during germination and early seedling development. Even though sufficient Fe was supplied, the Fe-responsive transcription factors ,, , and and their downstream targets and mediating Fe uptake were strongly upregulated in the mutant. Activation of the Fe deficiency response was confirmed by increased ferric chelate reductase activity in the mutant. At early stages, genes important for chloroplast redox control ( and ), Fe homeostasis ( and ), and chlorophyll metabolism ( and ) were downregulated, indicating limited Fe availability in plastids. In contrast, expression of, encoding a ferric reductase involved in Fe import into the mitochondria, was maintained, and Fe-dependent enzymes in the mitochondria were unaffected in Together, these data show that a failure to mobilize Fe stores during germination triggered Fe deficiency responses and strongly affected plastids, but not mitochondria.

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Mitochondrial iron transport and homeostasis in plants

Cited by 55 publications

References 66 publications

Retracted: Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess

Retracted: Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess

Comparative transcriptome analysis of nodules of two Mesorhizobium–chickpea associations with differential symbiotic efficiency under phosphate deficiency

Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds

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