Mitochondrial genome of Angiostrongylus vasorum: Comparison with congeners and implications for studying the population genetics and epidemiology of this parasite

Gasser, Robin B.; Jabbar, Abdul; Mohandas, Namitha; Schnyder, Manuela; Deplazes, Peter; Littlewood, D. Timothy J.; Jex, Aaron R.

doi:10.1016/j.meegid.2012.07.022

Cited by 36 publications

(44 citation statements)

References 46 publications

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“…The size of rrnS gene of A. mackerrasae was 696bp and the rrnL was 961bp. The size of both genes was identical to those described for A. vasorum 132 and Bunostomum trigonocephalum 137 and very similar to the size of rRNAs described previously for other nematodes (Table 5). costaricensis and A. vasorum) ( Table 7).…”

Section: Protein Genessupporting

confidence: 81%

“…1), similar in length to those of A. cantonensis (13,497 bp), A. costaricensis (13,585 bp) 133 and A.vasorum (13,422 bp) 132 .…”

Section: Characteristics Of Mt Genome Of a Mackerrasaementioning

confidence: 66%

“…Each protein coding mt gene was identified by local alignment comparison (performed in all six frames) using amino acid sequences from corresponding genes from mt genomes of A. vasorum, A. cantonensis and A. costaricensis; accession nos.NC_018602, GQ398121 and GQ398122, respectively 132,133 . The large and small subunits (rrnL and rrnS) of mt ribosomal RNA genes were identified by local alignment, and all transfer RNA (tRNA) genes were predicted and annotated based on available data from selected nematode superfamilies, (the Metastrongyloidea, Trichostrongyloidea, Ancylostomatidea and Strongyloidea).…”

Section: Sequencing and Data Analysesmentioning

confidence: 99%

“…As previously described for A. vasorum 132 , the two genes were separated from each other by protein-encoding genes, including nad3, nad5 and nad4L (Fig. 1).…”

Section: Protein Genesmentioning

confidence: 99%

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Molecular and pathological studies on Angiostrongylus species in southeast Queensland

Aghazadeh¹

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Despite an apparent increase in cases of angiostrongyliasis in humans and animals in Australia, the epidemiology of infection with the two species of Angiostrongylus that co-exist in this country, namely A. cantonensis and A. mackerrasae, is poorly understood. This knowledge gap is particularly important with respect to Angiostrongylus mackerrasae, a species evidently native to Australia, as its ability to cause disease in humans is unknown.Likewise, there is little information on the roles of native and introduced species of rodents and molluscs as hosts of Angiostrongylus species in Australia. This thesis focuses on the gaps in the knowledge about the epidemiology of two species in southeast Queensland, highlighting the hotspots for transmission of the parasite in urban Brisbane. A comprehensive survey of rats belonging to the species Rattus rattus, Rattus norvegicus and Rattus fuscipes was conducted. The prevalence of infection with Angiostrongylus spp. was 16.5% in Rattus spp. trapped in urban Brisbane and surrounds which is much higher than estimates of earlier studies. This highlights the possible risk of zoonotic infection in children, dogs and wildlife in this region and indicates the necessity for public awareness as well as more detailed epidemiological studies on this parasite in eastern Australia.Of the two Australian species, Angiostrongylus mackerrasae appears to be adapted to life in the native bush rat, Rattus fuscipes. This species evidently has an identical life cycle to that of Angiostrongylus cantonensis. A. mackerrasae may have greater potential to infect non-rodent hosts as it has been found as adult form in the lungs, as has been recorded for an individual flying fox.This raises concerns as to the potential of this native angiostrongylid to infect domestic animals and humans. To date, data on the taxonomy, epidemiology and population genetics of A. mackerrasae are poor or unknown. Here, we describe the mitochondrial (mt) genome of A. mackerrasae with the aim of addressing some of these knowledge gaps.The complete mitochondrial (mt) genome of A. mackerrasae was amplified from a single morphologically identified adult worm, by long-PCR in two overlapping amplicons (8 kb and 10 kb). The amplicons were sequenced using the MiSeq Illumina platform and annotated using an in-house pipeline. Amino acid sequences inferred from individual protein coding genes of the mt genomes were concatenated and then subjected to phylogenetic analysis using Bayesian inference.The mt genome of A. mackerrasae is 13,640 bp in size and contains 12 protein coding genes (cox1-3, nad1-6, nad4L, atp6 and cob), and two ribosomal RNA (rRNA) and 22 transfer RNA (tRNA) genes. The mt genome of A. mackerrasae has similar characteristics to those of other Angiostrongylus species which is explained in detail in chapter 3. Phylogenic comparisons reveal III that A. mackerrasae is closely related to A. cantonensis and the two sibling species may have recently diverged compared with all other species in the genus with a highl...

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Section: Protein Genessupporting

confidence: 81%

“…1), similar in length to those of A. cantonensis (13,497 bp), A. costaricensis (13,585 bp) 133 and A.vasorum (13,422 bp) 132 .…”

Section: Characteristics Of Mt Genome Of a Mackerrasaementioning

confidence: 66%

Section: Sequencing and Data Analysesmentioning

confidence: 99%

“…As previously described for A. vasorum 132 , the two genes were separated from each other by protein-encoding genes, including nad3, nad5 and nad4L (Fig. 1).…”

Section: Protein Genesmentioning

confidence: 99%

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Molecular and pathological studies on Angiostrongylus species in southeast Queensland

Aghazadeh¹

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“…These mitochondrial markers may be powerful to understand whether the different clinical forms of angiostrongylosis are caused by specific genetic variants of A. vasorum. 118 The recent exploration of the A. vasorum transcriptome predicted molecules essential in metabolic and biological processes, in host-immune responses as well as in drug targets and drug candidates. The most highly transcribed …”

Section: Innovative Insightsmentioning

confidence: 99%

Canine angiostrongylosis: recent advances in diagnosis, prevention, and treatment

Cesare¹,

Traversa²

2014

VMRR

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Angiostrongylus vasorum is a parasitic nematode affecting the heart and pulmonary arteries of wild (eg, foxes) and domestic canids. The parasite has an indirect life cycle in which slugs and snails act as intermediate hosts.In the last few years the parasite has spread outside the traditional endemic foci, and there is a rise of documented cases of canine angiostrongylosis across Europe. Angiostrongylus vasorum causes cardiopulmonary disorders and coagulopathies, along with different nonspecific clinical signs. Fatal infections are frequently reported. Given the severity of the infection and the recent geographic spreading of the parasite, this article reviews and discusses the current knowledge of A. vasorum, with a special focus on recent insights on diagnosis, prevention, and treatment of dog angiostrongylosis.

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Characterisation of the mitochondrial genome of Parafilaroides normani (lungworm) of Arctocephalus pusillus doriferus (Australian fur seal)

2014

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Parafilaroides normani is a metastrongyloid nematode of clinical relevance in some species of pinnipeds. The taxonomy, biology and epidemiology of this and related species are not well understood. Mitochondrial (mt) DNA can provide markers for studies in these areas, but genetic data are scant. Here, we characterised the mt genome of P. normani. Adults of this nematode were collected from Arctocephalus pusillus doriferus from Phillip Island, Victoria, Australia, and genomic DNA was extracted from individuals. The complete mt genome was amplified from DNA from a single adult male by long-range PCR, as two overlapping amplicons (~5 and ~10 kb), and then sequenced employing 454 technology (Roche). The mt genome was assembled from reads of ~300 bp and annotated using an established bioinformatic pipeline. This circular mt genome is 13,411 bp and contains two ribosomal RNA, 12 protein-coding and 22 transfer RNA genes, consistent with those of other lungworms studied to date. This mt genome is intended to provide a source of genetic markers for future investigations of the biology, epidemiology and systematics of Parafilaroides spp. and related lungworms.

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Mitochondrial genome of Angiostrongylus vasorum: Comparison with congeners and implications for studying the population genetics and epidemiology of this parasite

Cited by 36 publications

References 46 publications

Molecular and pathological studies on Angiostrongylus species in southeast Queensland

Molecular and pathological studies on Angiostrongylus species in southeast Queensland

Canine angiostrongylosis: recent advances in diagnosis, prevention, and treatment

Characterisation of the mitochondrial genome of Parafilaroides normani (lungworm) of Arctocephalus pusillus doriferus (Australian fur seal)

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