Mitochondrial genome of a flashwing demoiselle,Vestalis melaniafrom the Philippine Archipelago

Abstract

This study determined the first complete mitochondrial genome of a demoiselle, Vestalis melania (Odonata, Zygoptera, Calopterygidae) using long-range PCR and a primer walking approach. This mitogenome is 16,685 bp long and contains the entire set of 37 genes and an A + T-rich control region typically found in insects. Presently, this mitogenome is the largest mitogenome of all available odonates, mainly because of its long A + T-rich region (2036 bp). The gene arrangement of the V. melania mitogenome is identical to that of other known odonates. The inter-genic spacer s5 shared by the Anisoptera is absent in V. melania, which supports the view that the absence of the s5 spacer is a synapomorphy of the Zygoptera.

The complete mitochondrial genome of the neotropical helicopter damselflyMegaloprepus caerulatus(Odonata: Zygoptera) assembled from next generation sequencing data

“…In all observed parameters, the presented mitochondrial genome shows a strong similarity to all other already published odonate mitochondrial genomes (e.g. Lorenzo-Carballa et al 2014;Tang et al 2014;Yu et al 2014;Chen et al 2015;Feindt et al 2016;Herzog et al 2016). It contains the common arrangement of 37 genes including 13 protein-coding genes, 2 rRNA (16S and 12S rRNA) genes, 22 tRNA genes, and an A þ T rich (control) region of 1376 bp in length.…”

The complete mitochondrial genome of the emperor dragonflyAnax imperatorLEACH, 1815 (Odonata : Aeshnidae) via NGS sequencing

“…It exhibits the standard metazoan gene content of 37 genes, comprising 13 protein-coding genes, 22 tRNA genes, and two rRNA genes which are identically arranged as in the few other odonate mitochondrial genomes (e.g. Simon & Hadrys 2013;Lorenzo-Carballa et al 2014;Chen et al 2015;Yu et al 2014;. Overall base frequency is 76.0% AT-biased, for the 1291 bp long control (A þ T rich) region even 93.5%.…”

“…For mitochondrial genome annotation, the MITOS WebServer (mitos.bioinf.uni-leipzig.de/index.py) was applied and results were checked manually using BLAST (Altschul et al 1990) and available odonate mitochondrial genomes (e.g. Yu et al 2014;Chen et al 2015). Transfer RNA genes were predicted using both, the tRNAscan-SE v.1.21 Search Server (Lowe & Eddy 1997) and ARWEN v.1.2 (Laslett & Canb€ ack 2008).…”

Complete mitochondrial genomes of two damselfly species in coenagrionidae and phylogenetic implications

“…In order to determine the phylogenetic relationship of A. femina and I. senegalensis in Coenagrionidae, we collected 22 other mitogenome sequences from GenBank in Zygoptera (Lin et al 2010;Lorenzo-Carballa et al 2014;Chen et al 2015;Feindt et al 2016;Okuyama and Takahashi 2017;Zhang et al 2017;Xu et al 2018;Lan et al 2019;Song et al 2019 contaminata (Yu et al 2016)). We reconstructed the Maximum-likelihood tree by iqtree 2.1.2 (Nguyen et al 2015) (Figure 1).…”