Mitochondrial genetic variation within and between<i>Holbrookia lacerata lacerata</i>and<i>Holbrookia lacerata subcaudalis</i>, the spot-tailed earless lizards of Texas

Roelke, Corey E.; Maldonado, Jose A.; Pope, Blake W.; Firneno, Thomas J.; LaDuc, Travis J.; Hibbitts, Toby J.; Ryberg, Wade A.; Rains, Nathan D.; Fujita, Matthew K.

doi:10.1080/00222933.2018.1436726

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…Here we have found significantly more divergence (0.73%) between the Northern and Southern Spot-Tailed Earless Lizard using conserved orthologous sequences than previous study has estimated between two allopatric species using nuclear genes [52]. The results in this study, alongside Roelke et al (2018) finding that both the northern and southern lizards are genetically distinct using mitochondrial genomes, all further support the Hibbitts et al (2019) recent reclassification of their taxonomy. The elevation of the Northern and Southern Spot-Tailed Earless Lizard from subspecies to full species status requires the FWS consider them separately for protection, as each species faces unique threats [23,24].…”

Section: Pairwise Distancessupporting

confidence: 85%

“…The continuing reduction of per-base costs of high-throughput sequencing (HTS) methodologies has provided new opportunities to generate large-scale genomic and transcriptomic resources. This is especially beneficial for data deficient, non-model organisms that may require conservation action, are commercially valuable, or are excellent systems to investigate ecological and evolutionary questions [1][2][3][4]. The field of conservation genetics has historically relied on the use of few dozen genetic markers (allozymes, microsatellites, mitochondrial DNA, and a few nuclear genes) to understand population structure, viability, and evolutionary processes (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, two subspecies of H. lacerata were recognized, H. l. lacerata and H. l. subcaudalis, that are geographically separated by the Balcones Escarpment fault line. Renewed interest in resolving their taxonomic status revealed that the disjunct populations are reciprocally monophyletic with an approximately 4% mitochondrial sequence divergence [3]. Most recently, Hibbits et al (2019) took an integrative approach and used morphology, mitochondrial, and nuclear data to elevate each subspecies to full species status [23].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome sequencing reveals signatures of positive selection in the Spot-Tailed Earless Lizard

et al. 2020

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The continual loss of threatened biodiversity is occurring at an accelerated pace. Highthroughput sequencing technologies are now providing opportunities to address this issue by aiding in the generation of molecular data for many understudied species of high conservation interest. Our overall goal of this study was to begin building the genomic resources to continue investigations and conservation of the Spot-Tailed Earless lizard. Here we leverage the power of high-throughput sequencing to generate the liver transcriptome for the Northern Spot-Tailed Earless Lizard (Holbrookia lacerata) and Southern Spot-Tailed Earless Lizard (Holbrookia subcaudalis), which have declined in abundance in the past decades, and their sister species, the Common Lesser Earless Lizard (Holbrookia maculata). Our efforts produced high quality and robust transcriptome assemblies validated by 1) quantifying the number of processed reads represented in the transcriptome assembly and 2) quantifying the number of highly conserved single-copy orthologs that are present in our transcript set using the BUSCO pipeline. We found 1,361 1-to-1 orthologs among the three Holbrookia species, Anolis carolinensis, and Sceloporus undulatus. We carried out dN/dS selection tests using a branch-sites model and identified a dozen genes that experienced positive selection in the Holbrookia lineage with functions in development, immunity, and metabolism. Our single-copy orthologous sequences additionally revealed significant pairwise sequence divergence (~.73%) between the Northern H. lacerata and Southern H. subcaudalis that further supports the recent elevation of the Southern Spot-Tailed Earless Lizard to full species.

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Section: Pairwise Distancessupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome sequencing reveals signatures of positive selection in the Spot-Tailed Earless Lizard

et al. 2020

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“…However, in study systems where lineage history is reticulate rather than bifurcate, models with a phylogenetic network (rather than a tree) more appropriately account for evolutionary history. To estimate the history of diversification and hybridization of the five species of Aspidoscelis, we sequenced mitochondrial genomes (following Roelke et al 2018) and downloaded available mitochondrial sequence data from GenBank (Table S2). We used several software packages to estimate the phylogenetic network (Than and Nakhleh, 2008;Nguyen et al 2015;Solís-Lemus et al 2017); we provide details in the Supplementary Methods.…”

Section: Phylogenetic Network Estimationmentioning

confidence: 99%

Reduced Mitochondrial Respiration in Hybrid Asexual Lizards

Klabacka

Parry

Yap

et al. 2022

The American Naturalist

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The scarcity of asexual reproduction in vertebrates alludes to an inherent cost. Several groups of asexual vertebrates exhibit lower endurance capacity (a trait predominantly sourced by mitochondrial respiration) compared to congeneric sexual species. Here we measure endurance capacity in five species of Aspidoscelis lizards and examine mitochondrial respiration between sexual and asexual species using mitochondrial respirometry. Our results show reduced endurance capacity, mitochondrial respiration, and phenotypic variability in asexual species compared to parental sexual species along with a positive relationship between endurance capacity and mitochondrial respiration. Results of lower endurance capacity and lower mitochondrial respiration in asexualAspidoscelis are consistent with hypotheses involving mitonuclear incompatibility.

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“…, Eurasia(Agarwal et al 2018;Ceríaco and Bauer 2018;Grismer et al 2018), Oceania(Ruane et al 2018), North America(Cox et al 2018;Meik et al 2018;Roelke et al 2018), and South America(Kok et al 2018;Torres-Carvajal et al 2018)evidence that biodiversity research in squamates is global.…”

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confidence: 99%