Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity

Zhang, Guowei; Wang, Yang; Zou, Peng; Fan, Jun; Zeng, Yingfei; Chen, Qing; Sun, Lei; Yang, Huan; Zhou, Nina; Wang, Xiaogang; Liu, Jinyi; Cao, Jia; Zhou, Ziyuan; Ao, Lin

doi:10.1093/humrep/dey335

Cited by 69 publications

(58 citation statements)

References 49 publications

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“…This could explain the lower ORP values found in the V + E group, a treatment that provides a greater scavenging capacity. Zhang et al (2019) reported that spermatozoa with low MMP had a lower AR rate, which is consistent with our findings in the V + E group where we observed a better preservation of the acrosome and low spontaneous AR. The reason of this may be the low concentration of ROS, since the plasma membrane is highly susceptible to these highly reactive molecules due to the specific composition of the plasma membrane, rich in polyunsaturated fatty acids, which are structurally unstable and prone to lipid peroxidation (Agarwal & Dan Bui, 2017).…”

Section: Discussionsupporting

confidence: 93%

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant

Coria-Gómez¹,

Torres-Rodríguez²,

Villar-Muñoz³

et al. 2021

Andrologia

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The cryopreservation of spermatozoa has the main purpose of preserving male fertility. However, current preservation techniques have shown to produce lesions in the structure and alter sperm functions, probably due to the production of reactive oxygen species (ROS) during cryopreservation. To overcome the damage provoked by ROS, we introduced a novel antioxidant called EmbryORP® in a vitrification protocol and compared eight fertility parameters: motility, viability, morphology, concentration, the semen pH, the oxidation‐reduction potential (ORP), the spontaneous acrosomal reaction (AR) and the mitochondrial membrane potential (MMP), in the presence or absence of EmbryORP®. We analysed 20 samples from healthy human sperm donors and observed that the antioxidant significantly decreased the semen pH as well as the MMP and the ORP affecting the balance of ROS. The antioxidant also lowered the motility and viability of the cells, but preserved the acrosome and sperm morphology in general. We concluded that EmbryORP® lowered the ORP, but to a suboptimal level that may be harmful to spermatozoa. Despite these results, our work opens new perspectives on how to improve cryopreservation media. Therefore, we recommend exploring the EmbryORP® potential benefit by reducing its concentration or changing the exposure time during the cryopreservation protocol.

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Section: Discussionsupporting

confidence: 93%

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant

Coria-Gómez¹,

Torres-Rodríguez²,

Villar-Muñoz³

et al. 2021

Andrologia

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“…The functional capabilities of spermatozoa can be correlated with mitochondrial functions, which are reflected by intact MMP (Agnihotri et al, 2016). Spermatozoa with low MMP are less capable of undergoing the acrosome reaction (Gallon, Marchetti, Jouy, & Marchetti, 2006;Zhang et al, 2019). Mitochondrial functionality by way of an intact MMP seems to be a pre-requisite in maintaining acrosin activity, acrosome reaction and chromatin integrity in human spermatozoa.…”

Section: Mitochondrial Memb R Ane P Otential a S An Ind I C Ator Ofmentioning

confidence: 99%

“…Mitochondrial functionality by way of an intact MMP seems to be a pre-requisite in maintaining acrosin activity, acrosome reaction and chromatin integrity in human spermatozoa. Moreover, the dissipation of sperm MMP resulted in an overproduction of ROS along with decreased ATP levels (Zhang et al, 2019).…”

Section: Mitochondrial Memb R Ane P Otential a S An Ind I C Ator Ofmentioning

confidence: 99%

Causes and consequences of sperm mitochondrial dysfunction

et al. 2020

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Mitochondria are multifunctional organelles found in most eukaryotic organisms. The mitochondrion forms a widespread network in many cells, which are dynamically regulated by a cascade of processes that are closely related albeit independent (Wu, Zhang, & Ren, 2019). The mitochondrion is referred to as the cell's powerhouse, as it supplies most of the cell's chemical energy in the form of adenine triphosphate (ATP). Besides generation of ATP, mitochondria play a key role in numerous basic and advanced cellular processes, including cellular homeostasis and apoptosis (Herst, Rowe, Carson, & Berridge, 2017). They are also essential for cellular signalling and communication through various means, including reactive oxygen species (ROS). The mitochondrial electron transport chain (ETC) induces the production of ROS required in signalling pathways, but if produced in excess, can cause oxidative damage (St John, Bowles, & Amaral, 2007). Although almost all of the cytoplasm is removed during spermatogenesis, mitochondria are retained in mature spermatozoa, which suggest its importance in male fertility (Zhang et al., 2019). Spermatozoa contain a limited number of mitochondria in the midpiece, which play a vital role in sperm function. The energy required for spermatozoa to carry out cellular processes necessary for successful fertilisation such as motility, hyperactivation,

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“…Clinical trials showed that the mtDNA of oligo-asthenozoospermic patients present some defects that made DNA unavailable for amplification 28 and that large-scale deletions of mtDNA may be genetic risk factors for poor sperm quality in asthenoteratozoospermia-induced male infertility 29 . Numerous studies on humans pointed the importance of mitochondrial membrane potential not only for spermatozoa functionality 30 – 32 but also, in combination with sperm DNA fragmentation, as a superior to standard semen parameters for the prediction of natural conception 33 . It has been shown that TFAM is associated with the reduction in mtDNA content of human sperm 34 and that TFAM gene expression positive correlate with abnormal forms, sperm DNA fragmentation and mtDNA copy number 35 , 36 .…”

Section: Introductionmentioning

confidence: 99%

Reduced spermatozoa functionality during stress is the consequence of adrenergic-mediated disturbance of mitochondrial dynamics markers

Starovlah

Pletikosic

Kostic

et al. 2020

Sci Rep

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Here we investigate the stress-signaling responsible for the effects of acute/repeated psychological stresses (the most common stresses in human society) on spermatozoa number and functionality, as well as the transcriptional profile of mitochondrial dynamics markers by using the in vivo and ex vivo approaches. Acute and repeated stress inhibit spermatozoa functionality (acute –> 3.2-fold, repeated –> 2.5-fold), while only repeated stress reduces the spermatozoa number (1.7-fold). Stress hormones mimic these effects and decrease the spermatozoa functionality (adrenaline: 10 µM –> 2.4-fold, 100 µM – > 2.8-fold; hydrocortisone: 50 pM –> 2.7-fold, 500 pM –> 8.5-fold). They also significantly disturb the transcriptional profile of all main mitochondrial dynamics markers in spermatozoa. Ex vivo manipulation of stress signaling in spermatozoa reveals that most of these effects are mediated through ɑ1-and/or-β-adrenergic receptors. The transcription of these receptors and their kinases in the same samples is under the significant influence of adrenergic signaling. Our results are the first to show the importance of mitochondrial dynamics markers in spermatozoa since the transcriptional profiles of sixteen-out-of-ninteen are disturbed by manipulation of stress-hormones-signaling. This is a completely new molecular approach to assess spermatozoa functionality and it is important for a better understanding of the correlations between stress, environmental-life-style and other factors, and male (in)fertility.

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Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity

Cited by 69 publications

References 49 publications

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant

Causes and consequences of sperm mitochondrial dysfunction

Reduced spermatozoa functionality during stress is the consequence of adrenergic-mediated disturbance of mitochondrial dynamics markers

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Mitochondrial functionality modifies human sperm acrosin activity, acrosome reaction capability and chromatin integrity

Cited by 69 publications

References 49 publications

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ® : A novel antioxidant

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ® : A novel antioxidant

Causes and consequences of sperm mitochondrial dysfunction

Reduced spermatozoa functionality during stress is the consequence of adrenergic-mediated disturbance of mitochondrial dynamics markers

Contact Info

Product

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Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant

Comparative study of fertility parameters in vitrified human spermatozoa in the presence or absence of EmbryORP ^® : A novel antioxidant