Mitochondrial Function in Cell Wall Glycoprotein Synthesis in Saccharomyces cerevisiae NCYC 625 (Wild Type) and [ rho ⁰ ] Mutants

Abstract

We studied phosphopeptidomannans (PPMs) of two Saccharomyces cerevisiae NCYC 625 strains (S. diastaticus): a wild type strain grown aerobically, anaerobically, and in the presence of antimycin and a [rho 0] mutant grown aerobically and anaerobically. The aerobic wild-type cultures were highly flocculent, but all others were weakly flocculent. Ligands implicated in flocculation of mutants or antimycin-treated cells were not aggregated as much by concanavalin A as were those of the wild type. The [rho 0] mutants and antimycin-treated cells differ from the wild type in PPM composition and invertase, acid phosphatase, and glucoamylase activities. PPMs extracted from different cells differ in the protein but not in the glycosidic moiety. The PPMs were less stable in mitochondrion-deficient cells than in wild-type cells grown aerobically, and this difference may be attributable to defective mitochondrial function during cell wall synthesis. The reduced flocculation of cells grown in the presence of antimycin, under anaerobiosis, or carrying a [rho 0] mutation may be the consequence of alterations of PPM structures which are the ligands of lectins, both involved in this cell-cell recognition phenomenon. These respiratory chain alterations also affect peripheral, biologically active glycoproteins such as extracellular enzymes and peripheral PPMs.

Mitochondrial Associated Yeast Flocculation -The Effect of Acetylsalicylic Acid

“…This observation can be related to the presence of mitochondrial produced 3-OH oxylipins in strongly flocculating cells (i.e., 20 h) and their absence in weakly flocculent cells (i.e., 6 h) of this yeast 35 . This increase in mitochondrial activity towards the flocculent phase is in accordance with reports that suggest that mitochondrial function is important for flocculation 6,[9][10][11][16][17][18]27 . An opposite trend was evident in the non-flocculent S. cerevisiae UOFS Y-0122 control strain (Fig.…”

“…Thus, literature suggests that mitochondrial function and yeast flocculation is interrelated 11,21,33,35 . The importance of mitochondrial function in yeast flocculation has also been further demonstrated by the use of drugs (i.e., antimycin A, ethionine) that inhibit mitochondrial function 9,18,27 and by cells that carry deletions in mitochondrial genes 16 and petite (respiratory deficient) mutants 10,17 .…”

Enhancement of ethanol production in very high gravity fermentation by reducing fermentation-induced oxidative stress in Saccharomyces cerevisiae

“…These results suggest that the increased sensitivity of rho − mutant to these stresses might be caused by some other defects in this mutant. Previously, it has been reported that the rho mutation affects peripheral, biologically active glycoproteins (such as cell wall or extracellular enzymes) and structural glycoproteins (such as peripheral phosphopeptidomannans (PPMs)), leading to severe defects in cell wall biosynthesis and cell-cell recognition process 30 . In addition, a loss of mitochondrial DNA has been shown to result in unusual mitochondrial morphology 31 and defective intergenomic signaling, which functions in coordinating mitochondrial and nuclear gene expression 32 .…”

Cell Wall N-Linked Mannoprotein Biosynthesis Requires Goa1p, a Putative Regulator of Mitochondrial Complex I in Candida albicans

“…A link between mitochondrial metabolic activity and cell wall composition has been described briefly in S . cerevisiae [ 39 ]. Phosphopeptidomannans were less stable in mitochondrion-deficient cells than in wild type cells grown aerobically; a consequence of deficient mitochondria was a decreased cell flocculation [ 39 ].…”