2021
DOI: 10.1101/2021.02.11.430746
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Mitochondrial F1FOATP synthase determines the local proton motive force in cristae tips

Abstract: The classical view of oxidative phosphorylation is that a proton motive force PMF generated by the respiratory chain complexes fuels ATP synthesis. Under glycolytic conditions, ATP synthase in its reverse mode also can contribute to the PMF. Here, we dissected the two functions of ATP synthase and the role of the inhibitory factor 1 (IF1) under different metabolic conditions in detail. pH profiles of mitochondrial sub-compartments were recorded with high spatial resolution in live mammalian cells by positionin… Show more

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“…A recent paper has further confirmed one of the assertions of this present paper, and its patent application predecessors (e.g. [405–406]), that (to quote it) “IF1 is required to prevent the onset of ATP hydrolysis under OXPHOS conditions”, “IF1 is essential under OXPHOS conditions to prevent reverse ATP synthase activity”, “our data clearly show that IF1 is necessary to block ATP hydrolysis under OXPHOS conditions”, “to prevent futile ATP synthesis/hydrolysis cycles by reverse ATP synthase activity and ATP hydrolysis, IF1 is essential even and especially under OXPHOS conditions”, “IF1 is beneficial for ATP production even under OXPHOS conditions”, “ATP levels were lower in IF1-KO cells under OXPHOS conditions”, “clearly, this data demonstrates the importance of IF1 to block futile ATP hydrolysis under OXPHOS conditions” [407].…”
Section: Discussionmentioning
confidence: 99%
“…A recent paper has further confirmed one of the assertions of this present paper, and its patent application predecessors (e.g. [405–406]), that (to quote it) “IF1 is required to prevent the onset of ATP hydrolysis under OXPHOS conditions”, “IF1 is essential under OXPHOS conditions to prevent reverse ATP synthase activity”, “our data clearly show that IF1 is necessary to block ATP hydrolysis under OXPHOS conditions”, “to prevent futile ATP synthesis/hydrolysis cycles by reverse ATP synthase activity and ATP hydrolysis, IF1 is essential even and especially under OXPHOS conditions”, “IF1 is beneficial for ATP production even under OXPHOS conditions”, “ATP levels were lower in IF1-KO cells under OXPHOS conditions”, “clearly, this data demonstrates the importance of IF1 to block futile ATP hydrolysis under OXPHOS conditions” [407].…”
Section: Discussionmentioning
confidence: 99%