“…We therefore selected three genes for which primer sequences were available (see also Simon et al, 1994;Brower and DeSalle, 1994) and which we predicted were likely to be informative over a wide time period when analyzed together. Within insects, cytochrome b and the second expansion segment (also called divergent domains or variable regions) of the 28S ribosomal subunit have been used mainly to examine relatively recent divergences (Campbell et al, 1993;Pélandakis and Solignac, 1993;Crozier et al, 1995;Porter and Collins, 1996), while showing some conservation between distantly related taxa (Vossbrinck and Friedman 1989;Jermiin and Crozier, 1994;Schmitz and Moritz, 1994). In contrast, elongation factor-1a is a highly conserved gene (Friedlander et al, 1994), which, nevertheless, appears also to have some utility for recent divergences (Cho et al, 1995).…”