Mitochondrial DNA restriction fragment length polymorphisms in natural populations of Lentinula edodes

Evaluation of the Use of Outbred Lines for Screening of Genetic Markers in Shiitake (Lentinula edodes)

“…All the Japanese strains, including wild and commercial strains, formed one cluster. These results reflected the geographical distribution revealed from isozyme patterns, RFLPs of mtDNA, sequence data of rDNA (Ohmasa and Furukawa 1986, Fukuda et al 1994, Hibbett et al 1995. Among the Japanese strains, the range of similarity (Sxy = 1 − Dxy) was 0.60-0.79.…”

“…The Japanese strains of shiitake have been classified into a different clade from the New Zealand strains using restriction fragment length polymorphism (RFLP) of mtDNA (Fukuda et al 1994), and sequence analysis of rDNA (Hibbett et al 1995). Terashima et al (2002) and Kwan and Xu (2002) constructed genetic linkage maps of L. edodes using molecular markers.…”

Similarities in Restriction Fragment Patterns of Mitochondrial DNAs ofPhytophthoraSpecies Strongly Depend on the Restriction Enzyme Used Due to Heterogeneous Base Distribution and Sequence Conservation

“…It is generally assumed that restriction patterns of mtDNA can only be used for studies at or below the species level. It is commonly applied to study closely related species (Möller et al, 1993;Guillamón et al, 1994) or intraspecific variability (Hwang et al, 1991;Förster and Coffey, 1993;Fukuda et al, 1994;Lacourt et al, 1994;Varga et al, 1994). However, using the enzyme ScrFI we found 56% of the average number of bands to be present in all six Phytophthora species examined.…”

“…2 is a widely applied method in taxonomical and phylogenetic studies of different groups of true fungi (e.g., Bates et al, 1993;Fukuda et al, 1994, Guillamón et al, 1994Varga et al, 1994) as well as the oomycetes, of which Phytophthora has been studied in greatest detail (Förster et al, 1987(Förster et al, , 1988(Förster et al, , 1989(Förster et al, , 1990Förster and Coffey, 1993;Hwang et al, 1991;De Cock et al, 1992;Möller et al, 1993). The principle is simple: in the course of time base substitutions (point mutations) and insertions, deletions, inversions, and transpositions (macromutations) take place in mtDNA molecules.…”

Characterization of Subrepeat Regions within rDNA Intergenic Spacers of the Edible BasidiomyceteLentinula edodes

“…In many fungi, discrimination among inter-and intra-speciˆc strains has been achieved by random ampliˆed polymorphic DNA (RAPD), [24][25][26] arbitrarily primed polymerase chain reaction (AP-PCR), 27,28) restriction fragment length polymorphisms (RFLP) of mitochondrial DNA, 29,30) and sequence analysis of ITS. 2,4,5) In L. edodes, the analysis of 19 cultivated strains in China by AP-PCR, RAPD, and rDNARFLPs has shown their genetic homogeneity.…”