Mitochondrial DNA Rates and Biogeography in European Newts (Genus Euproctus)

Caccone, Adalgisa; Milinkovitch, Michel C.; Sbordoni, Valerio; Powell, Jeffrey R.

doi:10.2307/2413640

Cited by 39 publications

(51 citation statements)

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“…The null hypothesis of equal evolutionary rate throughout the tree was not rejected (with clock lnL = −1039.21, without clock lnL = −1023.49, p = .1411). This is in keeping with the rate constancy reported in interspecific studies of salamandrids (Caccone et al., ; Weisrock et al., ). In the absence of independent evidence to inform the calibration of a relaxed clock model (Arbogast, Edwards, Wakeley, Beerli, & Slowinski, ), a strict molecular clock was therefore used.…”

Section: Methodssupporting

confidence: 89%

“…15–9 Mya (Boccaletti et al., ), is generally used to infer the timing of the divergence of E . platycephalus from Euproctus montanus , the only other surviving member of the genus, which is endemic to Corsica (Caccone, Milinkovitch, Sbordoni, & Powell, ; Carranza & Amat, ; Miaud et al., ; Steinfartz, Veith, & Tautz, ; Zhang, Papenfuss, Wake, Qu, & Wake, ). Despite its long isolation, there is no morphological evidence for subspeciation of E. platycephalus .…”

Section: Introductionmentioning

confidence: 99%

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Islands within an island: Population genetic structure of the endemic Sardinian newt,Euproctus platycephalus

Ball

Bovero²,

Sotgiu³

et al. 2017

Ecology and Evolution

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The identification of historic and contemporary barriers to dispersal is central to the conservation of endangered amphibians, but may be hindered by their complex life history and elusive nature. The complementary information generated by mitochondrial (mtDNA) and microsatellite markers generates a valuable tool in elucidating population structure and the impact of habitat fragmentation. We applied this approach to the study of an endangered montane newt, Euproctus platycephalus. Endemic to the Mediterranean island of Sardinia, it is threatened by anthropogenic activity, disease, and climate change. We have demonstrated a clear hierarchy of structure across genetically divergent and spatially distinct subpopulations. Divergence between three main mountain regions dominated genetic partitioning with both markers. Mitochondrial phylogeography revealed a deep division dating to ca. 1 million years ago (Mya), isolating the northern region, and further differentiation between the central and southern regions ca. 0.5 Mya, suggesting an association with Pleistocene severe glacial oscillations. Our findings are consistent with a model of southward range expansion during glacial periods, with postglacial range retraction to montane habitat and subsequent genetic isolation. Microsatellite markers revealed further strong population structure, demonstrating significant divergence within the central region, and partial differentiation within the south. The northern population showed reduced genetic diversity. Discordance between mitochondrial and microsatellite markers at this scale indicated a further complexity of population structure, in keeping with male‐biased dispersal and female philopatry. Our study underscores the need to elucidate cryptic population structure in the ecology and conservation strategies for endangered island‐restricted amphibians, especially in the context of disease and climate change.

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Section: Methodssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Islands within an island: Population genetic structure of the endemic Sardinian newt,Euproctus platycephalus

Ball

Bovero²,

Sotgiu³

et al. 2017

Ecology and Evolution

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“…The Knersvlakte dates back to the upliftment of the great western escarpment during the Miocene, ≈ 18 Ma (Moon & Dardis 1988) and the best speculative estimate of the split between the Agama clades indicates a divergence at ≈ 715 000 years before present based on allozyme divergence (Flemming 1996). In contrast, however, using the suggested rate of 0.5–1.0%/Ma for 16 S rRNA data (Caccone et al . 1997) the time of divergence among individuals belonging to the respective clades is roughly 2.2–4.4 Myr before present.…”

Section: Discussionmentioning

confidence: 96%

Population fragmentation in the southern rock agama, Agama atra: more evidence for vicariance in Southern Africa

Matthee

Flemming

2002

Molecular Ecology

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Mitochondrial DNA sequence data derived from two genes were used to infer phylogeographical relationships between 13 Agama atra populations. Three distinct geographical assemblages were found among the lizard populations. The first occurs in southern Namibia, the second is restricted to the western dry arid regions of South Africa, whereas the third is distributed throughout the more mesic southern and eastern parts of the subcontinent. Geographically structured differences among populations within Agama clades are probably the result of dispersal and historic isolations among populations. At the broader scale, there were marked congruences between the Agama genetic discontinuities and those described previously in other rock-dwelling vertebrates such as Pronolagus rupestris and Pachydactylus rugosus. This suggests vicariance, probably as a response to natural climatic changes during the past three million years.

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“…N e can be calculated directly from θ if mutation rates are known. For salamanders, a large range of mtDNA substitution rates have been estimated (Tan and Wake 1995; Caccone et al 1997; Mueller et al 2004; Samuels et al 2005) but there is little published data on microsatellite mutation rates (although see Gopurenko et al 2006). Therefore, we estimated N e and N ef using a range of plausible mutation rates for both microsatellites and mtDNA to represent these uncertainties.…”

Section: Discussionmentioning

confidence: 99%

Inferring Population History and Demography Using Microsatellites, Mitochondrial Dna, and Major Histocompatibility Complex (Mhc) Genes

et al. 2008

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Microsatellites and mitochondrial DNA (mtDNA) have traditionally been used in population genetics because of their variability and presumed neutrality, whereas genes of the major histocompatibility complex (MHC) are increasingly of interest because strong selective pressures shape their standing variation. Despite the potential for MHC genes, microsatellites, and mtDNA sequences to complement one another in deciphering population history and demography, the three are rarely used in tandem. Here we report on MHC, microsatellite, and mtDNA variability in a single large population of the eastern tiger salamander (Ambystoma tigrinum tigrinum). We use the mtDNA mismatch distribution and, on microsatellite data, the imbalance index and bottleneck tests to infer aspects of population history and demography. Haplotype and allelic variation was high at all loci surveyed, and heterozygosity was high at the nuclear loci. We find concordance among neutral molecular markers that suggests our study population originated from post-Pleistocene expansions of multiple, fragmented sources that shared few migrants. Differences in N e estimates derived from haploid and diploid genetic markers are potentially attributable to secondary contact among source populations that experienced rapid mtDNA divergence and comparatively low levels of nuclear DNA divergence. We find strong evidence of natural selection acting on MHC genes and estimate long-term effective population sizes (N e ) that are very large, making small selection intensities significant evolutionary forces in this population.

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Mitochondrial DNA Rates and Biogeography in European Newts (Genus Euproctus)

Cited by 39 publications

References 0 publications

Islands within an island: Population genetic structure of the endemic Sardinian newt,Euproctus platycephalus

Islands within an island: Population genetic structure of the endemic Sardinian newt,Euproctus platycephalus

Population fragmentation in the southern rock agama, Agama atra: more evidence for vicariance in Southern Africa

Inferring Population History and Demography Using Microsatellites, Mitochondrial Dna, and Major Histocompatibility Complex (Mhc) Genes

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