Mitochondrial DNA IV. Interaction of ribopolynucleotides with the complementary strands of chick-liver mitochondrial DNA

Borst, Piet; Ruttenberg, G.J.C.M.

doi:10.1016/0005-2787(69)90089-6

Cited by 31 publications

(2 citation statements)

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“…co-workers concluded that rG-rich polymers bind to dC-rich clusters in DNA by Watson-Crick base pairing. Previous experiments from this laboratory on the binding of poly(rI,G), poly(rC) and poly(rI) to the separated strands of animal mtDNA are compatible with this interpretation [14,15]. By extrapolation, poly(rU) is considered to bind to dA-rich clusters in DNA, but a major objection to this interpretation is the absence of poly(rA) binding to the complementary dT-rich clusters that should be present on the other strand.…”

Section: Introductionsupporting

confidence: 60%

“…Binding of polyribonucleotides to single-stranded DNA in concentrated CsCl provides a convenient method to separate the complementary strands of many DNAs [1][2][3][4][5][6][7][8][9][10], because more of the polyribonucleotide is bound to one strand than to the other. Interest in the mechanism of polyribonucleotide binding was raised by the finding that binding often correlates with transcriptional specificity [11][12][13][14]. With many DNAs the coding DNA strand preferentially binds rG-rich polymers; its complement, rU-rich polymers.…”

Section: Introductionmentioning

confidence: 99%

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The binding of poly(rA) and poly(rU) to denatured DNA.I. Model studies with homopolymers.

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Borst

1976

Nucleic Acids Research

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We have compared the properties of the poly(rA).oligo(dT) complex with those of the poly(rU).oligo(dA)n complex. Three main differences were found. First, poly(rA) and oligo(dT)n do not form a complex in concentrations of CsCl exceeding 2 M because the poly(rA) is insoluble in high salt. If the complex is made in low salt, it is destabilized if the CsCl concentration is raised. Complexes between poly(rU) and oligo(dA)n, on the other hand, can be formed in CsCl concentrations up to 6.6 M. Second, complexes between poly(rA) and oligo(dT)n are more rapidly destabilized with decreasing chain length than complexes between poly(rU) and oligo(dA)n. Third, the density of the complex between poly(rA) and poly(dT) in CsCl is slightly lower than that of poly(dT), whereas the density of the complex between poly(rU) and poly(dA) in CsCl is at least 300 g/cm3 higher than that of poly(dA). These results explain why denatured natural DNAs that bind poly(rU) in a CsCl gradient usually do not bind poly(rA).

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Section: Introductionsupporting

confidence: 60%