Mitochondrial DNA genetic polymorphism in thirteen rice cytoplasmic male sterile lines

Abstract

Thirteen rice CMS lines derived from different cytoplasms were classified into eight groups by PCR amplification on mtDNA. The orf79 gene, which causes Boro II CMS, possibly results in Dian1-CMS. Thirteen rice cytoplasmic male sterile (CMS) lines derived from different cytoplasms are widely used for hybrid rice breeding. Based on 27 loci on mitochondrial DNA, including single nucleotide polymorphisms and segmental sequence variations between typical indica and japonica as well as high-polymorphism segmental sequence variations and single nucleotide polymorphisms among rice CMS lines, the 13 rice CMS lines were classified into eight groups: (I) wild-abortive CMS, Indonesian Shuitiangu CMS, K-CMS, Gang CMS, D-CMS and dwarf abortive CMS; (II) Maxie-CMS; (III) Honglian CMS; (IV) Boro II CMS; (V) Dian1-CMS; (VI) Liao-CMS; (VII) Lead CMS; and (VIII) Chinese wild rice CMS. According to their pollen abortion phenotypes, groups I and II (including 7 CMS lines) were classified as sporophytic CMS lines, the cytoplasmic genetic relationships among which were very close. They could have originated from similar, or even the same, cytoplasm donors. Groups III-VIII (including 6 CMS lines) were categorized as gametophytic CMS lines, the cytoplasms of which differed from one another, with some having relatively far genetic relationships. Dian1-CMS was found to harbor the orf79 gene, which causes Boro II CMS, whereas Liao-CMS had an orf79 structure that does not result in Lead CMS. Therefore, we speculated that orf79 is associated with Dian1-CMS but not with Liao-CMS. The atp6-orf79 structure related to sterility was also found to experience multiple evolutionary turnovers. All sporophytic CMS lines were indica-like. Except the Honglian CMS line, which was indica-like, all gametophytic CMS lines were japonica-like.

Comparative studies of mitochondrial proteomics reveal an intimate protein network of male sterility in wheat (Triticum aestivumL.)

“…More recently, with the mitochondrial genome sequence completely elucidated in some higher plant species, there has been an increased application of genomic approaches to study anther development and pollen reproduction, for example in the monocotyledonous plants Oryza sativa (rice) ( Notsu et al , 2002 ), Zea mays (maize) ( Clifton et al , 2004 ), and Triticum species (wheat) ( Ogihara et al , 2005 ), and dicotyledonous plants such as Arabidopsis thaliana ( Unseld et al , 1997 ), Brassica napus ( Handa, 2003 ), and Nicotiana tabacum (tobacco) ( Sugiyama et al , 2005 ). Many studies suggest that the mitochondrial genome is the carrier of fertility-related genes ( Hanson and Bentolila, 2004 ; Chen et al , 2010 ; Luo et al , 2013 ; Tang et al , 2014 ). Some of these genes and/or open reading frames (ORFs) encode pollen abortion-related proteins, such as ORF256 (7kDa) ( Song and Hedgcoth, 1994 ), ORF13 (13kDa) ( Klein et al , 2005 ), ORF138 (19kDa) ( Yasumoto et al , 2009 ), ORF288 (32kDa) ( Jing et al , 2012 ), ORF79 (8.9kDa) ( Wang et al , 2006 ; Luan et al , 2013 ), PCF (25kDa) ( Bentolila et al , 2002 ), and WA352 protein ( Luo et al , 2013 ; Tang et al , 2014 ).…”

Wide crossing diversify mitogenomes of rice

“…It is well documented that cytoplasmic inheritance in rice and most of the other higher plants is typically due to maternal inheritance. However, increasing reports of the complicated mitotypes in rice seems compromising this opinion [19,26]. In the present research, paternal mtDNA was not transmitted to the progenies ( Supplementary Table S7), but the mitotypes in BIL lines were strongly differentiated ( Fig.…”

Genetic characterisation and cytological identification of a male sterile mutant in maize (Zea mays L.)

“…Research in recent years shows that mtDNA is the genetic carrier (Hu et al 2014) for the occurrence of CMS, and that various plants in different types of cytoplasm contained mtDNA with distinct structures and sequences (Allen et al 2007;Alverson et al 2011;Chen and Liu 2014). At present, specific primers for identifying cytoplasmic types have been found in plants including rice (Luan et al 2013), maize (Slischuk et al 2011), rape (Zhao et al 2010), etc. By using mtDNA specific primers for PCR identification, this study found that the bands of K932MS were consistent with those of CMS-C Mo17 , further indicating that K932MS is a C-type cytoplasmic sterile mutant.…”