Mitochondrial DNA from yeast “petite” mutants: Specific changes of buoyant density corresponding to different cytoplasmic mutations

Mounolou, Jean‐Claude; Jakob, H; Słonimski, Piotr P.

doi:10.1016/0006-291x(66)90723-6

Cited by 221 publications

(52 citation statements)

References 13 publications

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“…For example, it was known that in yeast, chloramphenicol and other selective inhibitors of mitochondrial ribosomes, blocked the development of a functional cytochrome system but had no pronounced effect on most of the other enzymatic machinery of the organelle (4,5). A similar phenotype was found in cytoplasmic "petite" mutants of yeast (6) which are deficient in mitochondrial protein synthesis due to the loss of genetic information coding for mitochondrial ribosomal and transfer RNAs (7)(8)(9).…”

Section: In Vivo Studiessupporting

confidence: 61%

Mitochondrial Genes and Translation Products

Tzagoloff¹,

Macino²

1979

Annu. Rev. Biochem.

245

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Section: In Vivo Studiessupporting

confidence: 61%

Mitochondrial Genes and Translation Products

Tzagoloff¹,

Macino²

1979

Annu. Rev. Biochem.

245

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“…The relatively high concentration of cardiolipin in the diploid cytoplasmic petite yeast (YFAp) grown to log phase in galactose (Table V) particularly suggests that there is no major defect in cardiolipin synthesis in this mutant . It is of interest that the chromosomal petite, which as far as can be ascertained has qualitatively normal mitochondrial DNA (Mounolou, Jakob, and Slonimski, 1966), had a normal cardiolipin content when grown in the chemostat under maximally derepressed conditions . Thus, the genetic defect in this case probably does not S .…”

Section: Cells Grown In Chemostatmentioning

confidence: 99%

Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

Jakovcic

Getz

Rabinowitz

et al. 1971

The Journal of Cell Biology

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The phospholipid composition of various strains of the yeast, Saccharomyces cerevisiae, and several of their derived mitochondrial mutants grown under conditions designed to induce variations in the complement of mitochondrial membranes has been examined . Wild type and petite (cytoplasmic respiratory deficient) yeasts were fractionated into various subcellular fractions, which were monitored by electron microscopy and analyzed for cytochrome oxidase (in wild type) and phospholipid composition . 90% or more of the phospholipid, cardiolipin was found in the mitochondrial membranes of wild type and petite yeast . Cardiolipin content differed markedly under various growth conditions . Stationary yeast grown in glucose had better developed mitochondria and more cardiolipin than repressed log phase yeast . Aerobic yeast contained more cardiolipin than anaerobic yeast . Respiration-deficient cytoplasmic mitochondrial mutants, both suppressive and neutral, contained less cardiolipin than corresponding wild types . A chromosomal mutant lacking respiratory function had normal cardiolipin content . Log phase cells grown in galactose and lactate, which do not readily repress the development of mitochondrial membranes, contained as much cardiolipin as stationary phase cells grown in glucose . Cytoplasmic mitochondrial mutants respond to changes in the glucose concentration of the growth medium by variations in their cardiolipin content in the same way as wild type yeast does under similar growth conditions. It is concluded that cardiolipin content of yeast is correlated with, and is a good indicator of, the state of development of mitochondrial membrane .

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“…The ATPase of the entire FIFO-ATPase complex is sensitive to the antibiotic oligomycin and, like most proteins, is relatively stable at 0 "C. However, once F1 is detached from Fo, it becomes cold labile and its ATPase is no longer oligomycin sensitive. Together with Harvey Penefsky, I purified Fl from yeast mitochondria and showed that it could bind to Fo from beef heart mitochondria and regain its sensitivity to oligomycin When I returned to Vienna in the fall of 1966, Piotr Slonimski and others had just shown that the petite mutations of yeast profoundly altered the buoyant density of mitochondrial DNA, suggesting massive deletions (Mounolou et al, 1966). I decided to check whether these mutations also altered the mitochondrial ATPase complex.…”

mentioning

confidence: 99%

The hunt for mitochondrially synthesized proteins

Schatz

1997

Protein Science

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Mitochondrial DNA from yeast “petite” mutants: Specific changes of buoyant density corresponding to different cytoplasmic mutations

Cited by 221 publications

References 13 publications

Mitochondrial Genes and Translation Products

Mitochondrial Genes and Translation Products

Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

The hunt for mitochondrially synthesized proteins

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