Mitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts

Scott, Richard T.; Sun, Li; Zhan, Yiping; Marín, Diego; Tao, Xin; Seli, Emre

doi:10.1016/j.rbmo.2020.04.011

Cited by 33 publications

(32 citation statements)

References 18 publications

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“…These embryos have significantly higher levels of mtDNA compared to controls, thus suggesting a compensatory replicative mechanism to increase the number of normal copies necessary for embryo development [153]. However, the aforementioned findings that "the lower the mtDNA content, the better" has been refuted by other authors, who did not find any difference between blastocysts, regardless of ploidy, woman's age, or implantation potential [154][155][156]. Another study found that mtDNA quantitation did not distinguish between embryos that implanted and embryos that did not implant following a double embryo transfer (DET) [157].…”

Section: Mtdna and Trophectoderm Biopsymentioning

confidence: 98%

Embryo and Its Mitochondria

et al. 2021

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The mitochondria, present in almost all eukaryotic cells, produce energy but also contribute to many other essential cellular functions. One of the unique characteristics of the mitochondria is that they have their own genome, which is only maternally transmitted via highly specific mechanisms that occur during gametogenesis and embryogenesis. The mature oocyte has the highest mitochondrial DNA copy number of any cell. This high mitochondrial mass is directly correlated to the capacity of the oocyte to support the early stages of embryo development in many species. Indeed, the subtle energetic and metabolic modifications that are necessary for each of the key steps of early embryonic development rely heavily on the oocyte’s mitochondrial load and activity. For example, epigenetic reprogramming depends on the metabolic cofactors produced by the mitochondrial metabolism, and the reactive oxygen species derived from the mitochondrial respiratory chain are essential for the regulation of cell signaling in the embryo. All these elements have also led scientists to consider the mitochondria as a potential biomarker of oocyte competence and embryo viability, as well as a key target for future potential therapies. However, more studies are needed to confirm these findings. This review article summarizes the past two decades of research that have led to the current understanding of mitochondrial functions in reproduction

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Section: Mtdna and Trophectoderm Biopsymentioning

confidence: 98%

Embryo and Its Mitochondria

et al. 2021

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“…A final interesting group of oocyte abnormalities that may result in RPL relates to mitochondrial abnormalitiesboth alterations in mitochondrial number and function. Initial studies showed diminished outcomes with an increase in mitochondrial DNA content, but subsequent studies failed to find any predictive value to this measurement (32,33). Further studies focusing on patients with otherwise unexplained RPL are needed to resolve whether mitochondrial abnormalities are a marker of impaired reproductive potential, are causally involved in embryonic arrest, or are simply uncorrelated with clinical outcomes.…”

Section: The Oocytementioning

confidence: 99%

Role of the sperm, oocyte, and embryo in recurrent pregnancy loss

Klimczak

Patel

Hotaling

et al. 2021

Fertility and Sterility

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“…Fragouli and Ravichandran et al established a screening threshold which embryos with mtDNA content above the threshold was less likely to implant (29,30). Whereas subsequent studies have reached conflicting conclusions (31)(32)(33). These contradictory findings limit the application of mtDNA in the accurate assessment of embryo viability in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer

et al. 2021

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An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth. In the study, we investigated whether mtDNA content is a reliable screening biomarker for live birth after single blastocyst transfer. A total of 233 couples with 316 blastocyst stage embryos undergoing in vitro fertilization treatment and pre-implantation genetic testing analysis were included in the study. All embryos were chromosomally normal and had undergone single-embryo transfers. There was no significant difference observed in the blastocyst mtDNA content among the live birth, miscarriage and non-implanted groups (p=0.999), and the mtDNA content in blastocysts from the miscarriage and live birth groups was similar [median (interquartile range), 1.00*108(7.59*107- 1.39*108) vs 1.01*108 (7.37*107- 1.32*108)]. Similarly, no significant association was observed between mtDNA content and embryo implantation potential (p=0.965). After adjusting for multiple confounders in a logistic regression analysis with generalized estimating equations, no associations between mtDNA content and live birth were observed in all blastocysts, Day-5 and Day-6 blastocysts (p=0.567, p=0.673, p=0.165, respectively). The live birth rate was not significantly different between blastocysts with an elevated mtDNA content and blastocysts with a normal mtDNA content (26.7% vs 33.6% p=0.780). Additionally, there was no linear correlation between the mtDNA content and maternal age (p=0.570). In conclusion, the mtDNA content does not seem to be a potential biomarker for embryo transfer outcomes (i.e., implantation and live birth) based on the existing testing tools. Embryos with an elevated mtDNA content also have development potential for successful live birth.

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Mitochondrial DNA content is not predictive of reproductive competence in euploid blastocysts

Cited by 33 publications

References 18 publications

Embryo and Its Mitochondria

Embryo and Its Mitochondria

Role of the sperm, oocyte, and embryo in recurrent pregnancy loss

Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer

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