Mitochondrial DNA and chromosomal studies of wild mice (Mus) from Turkey and Iran

Abstract

Complete D-loop sequences of 20 Mus from three localities in Turkey and seven in Iran were characterized. These countries are thought to be close to the place of origin of the subspecies Mus musculus domesticus. Five new M. m. domesticus haplotypes were added to the nine already known for the region. Four of these 14 haplotypes were very similar to the consensus D-loop sequence for western Europe defined by Nachman et al. (1994), which may represent the ancestral condition for M. m. domesticus. A divergent mtDNA lineage is found in various parts of Turkey and northern Iran; it has spread into western Europe, but other European lineages were not found in either Turkey or Iran. The other Mus D-loop sequences were of M. m. castaneus and Mus macedonicus and confirmed M. macedonicus as a monotypic species with low nucleotide diversity. The prevalence of the standard 40-chromosome complement in this region is particularly interesting with regards M. m. domesticus, as it is consistent with the in situ origin of Robertsonian karyotypic races (2n < 40) in western Europe.

Of mice and the ‘Age of Discovery’: the complex history of colonization of theAzorean archipelago by the house mouse (Mus musculus) as revealed by mitochondrialDNAvariation

“…All sequence traces were checked for ambiguous bases in Sequencher v. 4.5 (Gene Codes Corp., Ann Harbor, MI, USA) and aligned by eye using Bioedit v7.1.3.0. (Hall, ) with7 520 previously published M. m. domesticus haplotypes downloaded from GenBank (Prager et al ., , , ; Nachman et al ., ; Gündüz et al ., , , ; Ihle et al ., ; Rajabi‐Maham et al ., ; Förster et al ., ; Searle et al ., , b; Hardouin et al ., ; Jones et al ., , , b, ; Gabriel et al ., ) or directly from the publication (mouse sequences in Bonhomme et al ., ; from countries neighbouring the Atlantic; Gray et al ., ). All sequences resulting in new D‐loop haplotypes were deposited in GenBank (accession numbers: KP178629–KP178668).…”

“…The fast‐evolving D‐loop is by far the most extensively studied molecular marker in house mouse colonization studies (Prager et al ., , , ; Nachman et al ., ; Gündüz et al ., , , ; Ihle et al ., ; Rajabi‐Maham et al ., ; Förster et al ., ; Searle et al ., , b; Hardouin et al ., ; Jones et al ., , , b, ; Bonhomme et al ., ; Gabriel et al ., ; Gray et al ., ). There is evidence that the D‐loop is particularly good at revealing initial colonization of an area, with the marker (being maternally inherited) reflecting a lack of penetrance of later‐arriving female mice into pre‐existing populations (Förster et al ., ; Gabriel et al ., ; Bonhomme & Searle, ).…”

The diverse origins of New Zealand house mice

“…The complete mt D-loop and flanking regions were amplified with the PCR primers and conditions as described in Gü ndü z et al (2000) or with the primer pairs L15320/ H15782 and L15735/H00072 of Prager et al (1993). The fragments generated were sequenced in both directions using the same primers.…”

“…The phylogenetic analysis of M. m. domesticus is the same as that reported in the companion paper (Searle et al 2009) (Prager et al 1996;Gü ndü z et al 2000) and we adopted those approaches here as well. The NJ tree was also based on the substitution model selected by MODELTEST with bootstrap values from 10 000 replicates.…”

Ege Denizi ve Marmara Denizi’ndeki adalardan Türkiye kemirici faunasının karyolojisine ve yayılışına katkılar

“…Standard karyotype including 40 acrocentric chromosomes has been reported for M. domesticus and M. macedonicus from Central Anatolia, Western Black Sea and Eastern Anatolia in Turkey (Gündüz et al 2000b;Gözcelioğlu et al 2005;. However, the karyotype with 38 chromosomes including a metacentric chromosome pair arising from Robertsonian fusion has been detected in a population of M. macedonicus from Western Anatolia (Gündüz et al 2000a).…”