Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria

Agbebi, O. T.; Echefu, C; Adeosun, Ifeoluwa Rachael; Ajibade, A.J.; Adegbite, EA; Adebambo, A.O.; Ilori, Matthew O.; Durosaro, Samuel O.; Ajibike, A. B.

doi:10.9734/bbj/2016/25470

Cited by 7 publications

(6 citation statements)

References 15 publications

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“…Hence, there is urgent need for conservation. This finding is in line with the report of Agbebi et al (2016) who observed low nucleotide diversity in all the four Tilapia species studied. Babatunde et al (2016) also reported a low level of nucleotide diversity in Nigerian locally adapted chickens.…”

Section: Discussionsupporting

confidence: 93%

Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis

et al. 2020

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Abstract. Ukenye E, Megbowon I, Oguntade O, Oketoki T, Amusa O, Usman A, Sokenu B, Adeleke R, Joseph B, Omatah C. 2020. Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis Biodiversitas 21: 5317-5321. Tilapia guineensis is an important cichlid species of West African coastal waters with good nutritional, economic, and aquaculture relevance. The knowledge of the genetic basis of variation in growth traits in Tilapia fish is of great importance to support genetic improvement in the context of aquaculture. In this study, regions of the Tilapia guineensis IGF-1 gene were sequenced, aligned and compared across populations to identify single nucleotide polymorphism and genetic diversity among four populations of T. guineensis in South-west Nigerian coastal waters. A total of thirty-four SNPs were identified across the studied populations and were detected in the forward reaction with twenty-two transversions and twelve transitions. Badagry population showed the highest genetic diversity with the highest molecular diversity indices; number of polymorphic sites, pairwise differences, number of segregating sites and nucleotide diversity while the least diverse population was Pepe. Analysis of molecular variance (AMOVA) revealed that genetic variation was mostly within populations. This finding provides more information regarding variation in insulin growth factor I of T. guineensis and will encourage association study for production traits that will inform useful selection marker for breeding program.

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Section: Discussionsupporting

confidence: 93%

Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis

et al. 2020

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“…The findings here corroborate the earlier report by [22] that populations of tilapia species in Nigeria showed no evidence of sub-structuring in their phylogeny but may be traced to a single ancestral lineage. Similarly, phylogenetic analysis by [35] and [36] also revealed population-based phylogenetic clustering of fish.…”

Section: Discussionsupporting

confidence: 92%

“…ward (5'-GGATTYTAACCCYTRCCCC-3') and Czilli Reverse (3'-AGTAAAG TCAGGACCAAGCC-5') for D-loop region as previously used by [22]. Czilli Forward (5'-GGATTTTAACCCTTACCCC-3') and Czilli Reverse (3'-AGTAAAGTCAGGACCAAGCC-5') were designed and used for cytochrome b region of the mtDNA.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Phylogenetics and Molecular Divergence of Tilapia Fish (Oreochromis Species) Using Mitochondrial D-Loop and Cytochrome b Regions

Ekerette¹,

Ikpeme²,

Udensi³

et al. 2018

AJMB

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Understanding the level of genetic diversity in any population is an important requisite towards strategizing measures for conservation and improvement of stocks. This study focused on the assessment of phylogenetics and molecular divergence of tilapia fish species obtained from two populations (Domita in South-South and Odeda in South-West, Nigeria) using the displacement loop (D-loop) and cytochrome b region of the mitochondrial deoxyribonucleic acid (mtDNA). A total of 28 samples (15 from South-South and 13 from SouthWest) were used for the genetic analysis. DNA was extracted from the tissue of all the samples using Quik-gDNA TM miniPrep kit. The D-loop containing the hypervariable region was sequenced for all samples from the two populations, while cytochrome b (Cyt b) region of mtDNA was only sequenced for samples from South-South population. Chromatograms of the sequences were viewed and edited using Bioedit software. Multiple sequence alignment was carried out using molecular evolutionary genetic analysis (MEGA) software before subsequent genetic analyses. Phylogenetic analysis grouped the samples into two clusters based on population. Also, when the two mitochondrial regions were pooled together, they clustered into two major groups based on mitochondrial regions. Analysis of molecular variance (AMOVA) revealed 37.32% variation within population and 62.68% variation among population with a significant fixation index of 0.627 (p < 0.05).

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“…These high similarity levels may be a result of a major event like flooding which is common in Nigeria or escapes from restricted areas (farms). Oreochromis niloticus used in previous work to assess mitochondria diversity of some cichlids in Nigeria also showed varying levels of similarity 36 . However, varying levels of similarity could be an indication that the species is hybridizing with other tilapia species.…”

Section: Resultsmentioning

confidence: 92%

Genetic Similarity Levels in Nile Tilapia of Some Nigerian Water Bodies Using SSR Markers

T.O.¹,

Anumudu²

2023

J. of Fisheries and Aquatic Science

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Background and Objective: In Nigeria, there is a paucity of information on the genetic similarity among wild Oreochromis niloticus species. Though there are assertions based on morphological observation, by fish farmers and some researchers of fisheries that most of these species in the wild are no longer pure and possibly hybridizing. Most molecular assessments are usually among tilapia species with limited sample coverage of O. niloticus species or of a lower marker. This was a preliminary investigation of the genetic similarity of Oreochromis niloticus from selected water bodies along the River Niger, in six states of Nigeria, using SSR markers. Materials and Methods: Similarity levels among Oreochromis niloticus from water bodies in six states in Nigeria were assessed using seven SSR markers with their various primers (UNH 208, 143, 104, 022, 119, 180 and 156), which were selected from the NCBI database. Only UNH 104, UNH143 and UNH 022 produced DNA amplifications. Phylogenetic representation using UPGMA cluster analysis produced a dendrogram with a total of 118 clusters grouped into 7 major clusters with up to 8 subgroups, indicating different degrees of variation and similarities. Results: Genetic similarities were observed at various coefficient levels, possibly due to hybridization. At the coefficient level of 1 or 100%, 39 clusters occurred with different samples on each cluster indicating they are genetically the same. Samples from South Delta (SSD), South West Oyo (SWO) and North West Kebbi (NWK) states were always present in each cluster at this coefficient level. A lot of migration is likely taking place among these fishes. The seven major clusters A, B, C, D, E, F and G at varying degrees of similarity 73, 67, 64, 59, 56, 48 and 44% showed the decreasing order of genetic similarity. Conclusion: These data supported the view that the very high similarity between tilapia from these water bodies stems from its highly prolific nature which may have led to a high probability of hybridization between species and strains. The genetic distance between strains may suggest that there are varying levels of genetic variability among O. niloticus in Nigeria water bodies under study.

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Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria

Cited by 7 publications

References 15 publications

Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis

Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis

Phylogenetics and Molecular Divergence of Tilapia Fish (Oreochromis Species) Using Mitochondrial D-Loop and Cytochrome b Regions

Genetic Similarity Levels in Nile Tilapia of Some Nigerian Water Bodies Using SSR Markers

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