Mitochondrial damage are involved in Aflatoxin B1-induced testicular damage and spermatogenesis disorder in mice

Huang, Wanyue; Cao, Zheng; Yao, Qiucheng; Ji, Qiang; Zhang, Jian; Li, Yanfei

doi:10.1016/j.scitotenv.2019.135077

Cited by 48 publications

(26 citation statements)

References 63 publications

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“…Reductions in SOD, CAT and GSH-dependent enzymes (GST and GPx) activities and GSH levels in AFB1 alone-treated animals signify the inhibition of their antioxidant function which is related to the cellular RONS buildup and oxidative damage in the epididymis, testes, and hypothalamus of the rats. The current data substantiate earlier reports on AFB1-mediated stimulation of oxidative stress testes of rats, 15,16 although decreases in MDA and RONS levels with a simultaneous increase in the antioxidant enzymes activities in rats co-treated with AFB1 and GA are associated with the antioxidant effect of GA previously reported. 62…”

Section: Discussionsupporting

confidence: 93%

“…13 Moreover, data from experimental animals have demonstrated that AFB1 may be an essential risk factor for male infertility. 14,15 Earlier animal studies revealed that acute and chronic AFB1 exposure induced testicular damage disrupted endocrine function and spermatogenesis, leading to impaired fertility. 16,17 Elucidated mechanisms associated with AFB1-mediated reproductive toxicity include suppression of androgen biosynthetic proteins (17 β-hydroxysteroid dehydrogenase 3, 3β-hydroxysteroid dehydrogenase, and steroidogenic acute regulator), induction of oxidative stress, inhibition of antioxidant defense systems, germ cell apoptosis and autophagy.…”

Section: Introductionmentioning

confidence: 99%

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Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

Owumi

Adedara

Akomolafe

et al. 2020

Exp Biol Med (Maywood)

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Aflatoxin B1 (AFB1) is reported to elicit adverse reproductive outcomes in animals. Gallic acid (GA) is known to exhibit antioxidant and inflammatory bioactivities. The impact of GA on AFB1-facilitated reproductive dysfunction is nonexistent in literature. This investigation elucidated GA protective effect on AFB1-induced reproductive toxicities in rats, exposed for 28 consecutive days to AFB1 (75 µg/kg), or co-treated with GA (20 or 40 mg/kg) body weight. AFB1 significantly (p < 0.05) reduced testicular function biomarkers, serum hormonal levels, and functional sperm characteristics in experimental animals. GA abated AFB1-induced increases (p < 0.05) in lipid peroxidation and reactive oxygen and nitrogen species, suppressed myeloperoxidase, interleukin-1β, nitric oxide, and tumor necrosis factor-α levels—inflammatory biomarkers—in testes, epididymis, and hypothalamus. Furthermore, GA improved antioxidant defenses and alleviated reduction in interleukin-10, caspase-3 activation, and histological variations in epididymis, testes, and hypothalamus of rats dosed with AFB1. Conclusively, GA enhanced reproductive function in AFB1-exposed rats by modulating inflammatory, oxidative stress, and apoptosis mediators. Impact statement Infertility resulting from reproductive deficiency can be stressful. Exposure to aflatoxin B1, a dietary mycotoxin prevalent in improperly stored grains, is reported to elicit reproductive insufficiencies and infertility. We, therefore, examined the likely beneficial effect of gallic acid (GA) a phytochemical, recognized to exhibit in vitro and in vivo pharmacological bioactivities against oxidative stress and related inflammatory damages in rats, since AFB1 toxicities are predicated on oxidative epoxide formation, in a bid to proffer new evidence to advance the field of nutriceutical application from plant-derived chemopreventive agents. Our findings will advance the field of chemoprevention by presenting data absent in the literature on GA. Our results demonstrate further evidence for GA conferred protection against AFB1-mediated histological lesions in testes, epididymis, and hypothalamus of treated rats; suppresses oxidative damages, relieved inflammatory and apoptotic responses, restored sperm functional characteristics, and hormonal levels relevant for reproductive integrity and function.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

Owumi

Adedara

Akomolafe

et al. 2020

Exp Biol Med (Maywood)

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“…NRF1 can regulate TFAM , which is mainly involved in mitochondrial DNA replication and transcription ( Hock and Kralli, 2009 ). Huang et al (2020) reported that AFB 1 decreased ATP levels, reduced the activities of mitochondrial ETC I-IV, and downregulated mRNA expression of mitochondrial biogenesis genes, including PGC-1α, NRF1 , and TFAM in mice testes. Similarly, increased mitochondrial swelling, reduced ATP concentration, and decreased activities of ETC mitochondrial complexes I, II, III, and V were observed in the present study.…”

Section: Discussionmentioning

confidence: 98%

Protective effects of lycopene on mitochondrial oxidative injury and dysfunction in the liver of aflatoxin B1-exposed broilers

Wan

Chen

et al. 2021

Poultry Science

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This study was conducted to investigate the effects of lycopene ( LYC ) on mitochondrial oxidative injury and dysfunction in the liver of aflatoxin B 1 ( AFB 1 )-exposed broilers. A total of 192 healthy 1-day-old male broilers were randomly divided into 3 groups with 8 replicates of 8 birds each. Birds in the 3 groups were fed basal diet ( control ), basal diet with 100 µg/kg AFB 1 , and basal diet with 100 µg/kg AFB 1 and 200 mg/kg LYC, respectively. The experiment lasted 42 d. The results showed that AFB 1 decreased average daily body weight gain ( ADG ), average daily feed intake, and gain to feed ratio ( G :F ) compared to the control group, the LYC supplementation increased ADG and G/F compared to AFB 1 group ( P < 0.05). Broilers in the AFB 1 group had lower mitochondrial glutathione ( mGSH ) concentration and glutathione peroxidase ( GSH-Px ), manganese superoxide dismutase ( MnSOD ), and thioredoxin reductase activities, and higher hydrogen peroxide ( H 2 O 2 ) and reactive oxygen species ( ROS ) concentrations than the control group ( P < 0.05). The LYC increased mGSH concentration and GSH-Px and MnSOD activities, and decreased H 2 O 2 and ROS concentrations compared to AFB 1 group ( P < 0.05). Broilers fed the AFB 1 diet showed increased mitochondrial swelling and decreased adenosine triphosphate concentration than the control group, and LYC had opposite effects ( P < 0.05). The AFB 1 decreased the activities of mitochondrial electron transfer chain ( ETC ) complexes I, II, III, and V, downregulated the mRNA expression levels of hepatic MnSOD, thioredoxin 2, thioredoxin reductase, peroxiredoxin-3, peroxisome proliferator-activated receptor γ coactivator 1α, nuclear respiratory factor 1 , and mitochondrial transcription factor A compared with the control group ( P < 0.05), and LYC increased activities of mitochondrial ETC complexes III and V, and upregulated mRNA expression levels of these genes in comparison to AFB 1 group ( P < 0.05). In conclusion, the LYC protected broilers from AFB 1 -induced liver mitochondrial oxidative injury and dysfunction by stimulating mitochondrial antioxidant capacity and maintaining mitochondrial biogenesis.

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“…Quantitative real-time PCR (BioEasy SYBR Green High ROX Master Mix, Bioer, Hangzhou, China) was performed according to the methods described by Vinué et al (2015) . The relative expression levels of the tested genes were calculated using the 2 –ΔΔ CT method as described by Huang W. et al (2020) .…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Characteristic of Swine-Origin mcr-1-Positive Escherichia coli in Northeastern China

et al. 2021

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The emergence of the plasmid-mediated colistin resistance gene mcr-1 is threatening the last-line role of colistin in human medicine. With mcr-1-positive Escherichia coli (E. coli) isolated from food animal being frequently reported in China, the prevalence of mcr-1 in food animal has attracted public attention. In the present study, a total of 105 colistin-resistant E. coli strains were isolated from 200 fecal samples collected from six swine farms in northeastern China. mcr-PCR revealed that the prevalence of mcr-1 in colistin-resistant E. coli was 53.33% (56/105). mcr-1-positive E. coli showed extensive antimicrobial resistance profiles with the presence of additional resistance genes, increased expression of multidrug efflux pump-associated genes, and increased biofilm formation ability. MLST differentiated all the mcr-1-positive E. coli into 25 sequence types (STs) and five unknown ST, and the most common ST was ST10 (n = 11). By phylogenetic group classification, the distribution of all mcr-1-positive E. coli belonging to groups A, B1, B2, and D was 46.43, 35.71, 5.36, and 5.36%, respectively. Conjugation experiment demonstrated that most of the mcr-1 were transferable at frequencies of 2.68 × 10–6–3.73 × 10–3 among 30 representative mcr-1-positive E. coli. The plasmid replicon types IncI2 (n = 9), IncX4 (n = 5), IncHI2 (n = 3), IncN (n = 3), and IncP (n = 1) were detected in the transconjugants. The results of growth assay, competition experiment, and plasmid stability testing showed that acquisition of mcr-1-harboring plasmids could reduce the fitness of bacterial hosts, but mcr-1 remained stable in the recipient strain. Due to the potential possibility of these mcr-1-positive E. coli being transmitted to humans through the food chain or through horizontal transmission, therefore, it is necessary to continuously monitor the prevalence and dissemination of mcr-1 in food animal, particularly in swine.

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Mitochondrial damage are involved in Aflatoxin B1-induced testicular damage and spermatogenesis disorder in mice

Cited by 48 publications

References 63 publications

Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

Protective effects of lycopene on mitochondrial oxidative injury and dysfunction in the liver of aflatoxin B1-exposed broilers

Prevalence and Characteristic of Swine-Origin mcr-1-Positive Escherichia coli in Northeastern China

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