Mitochondrial Cytochrome B Variation in Populations of the Visceral Leishmaniasis Vector Lutzomyia Longipalpis Across Eastern Brazil

Abstract

A population analysis of peridomestic, light-trapped, field specimens of the phlebotomine sand fly Lutzomyia longipalpis was targeted to six locations representing a geographic transect across eastern Brazil. Mitochondrial cytochrome b gene sequences established the pattern of genetic variation among the populations. Alignment of a 261-basepair region at the 3Ј end of cytochrome b identified 30 haplotypes and 21 segregating sites from 78 sand flies. Pairwise comparisons indicated statistically significant population structuring between northern and southern populations, as well as structuring among the southern populations. Prominent spatial clustering was evident for two of the populations in a minimum spanning network of the haplotypes, but sequence divergence was not sufficient to indicate cryptic species.

Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review

“…According to Soto et al (2001), their results question the conspecific status of Brazilian populations. Arrivillaga et al (2002) and Hodgkinson et al (2003) reach a different conclusion, even though the latter study did find a significant differentiation between Northern and Southern populations in their analysis of mitochondrial cytochrome b gene sequences in six locations representing a geographic transect across Eastern Brazil.…”

“…longipalpis, analysis of mitochondrial DNA in three different studies (Soto et al 2001, Arrivillaga et al 2002, Hodgkinson et al 2003) gave somewhat ambiguous results. According to Soto et al (2001), their results question the conspecific status of Brazilian populations.…”

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

“…A total of 83 intraspecific studies has been recorded in the literature (Esseghir et al, 1997;Marcondes et al, 1997;Dias et al, 1998;Aransay et al, 2000Aransay et al, , 2003Di Muccio et al, 2000;Esseghir and Ready, 2000;Mukhopadhyay et al, 2000;Yin et al, 2000;Marquez et al, 2001;Soto et al, 2001;Arrivillaga et al, 2002Arrivillaga et al, , 2003Bauzer et al, 2002a,b;Depaquit et al, , 2004aDepaquit et al, , 2005bDepaquit et al, , 2008bDepaquit et al, , 2013Depaquit et al, , 2014Hodgkinson et al, 2002Hodgkinson et al, , 2003Testa et al, 2002;Bottecchia et al, 2004;Margonari et al, 2004;Pesson et al, 2004;Yahia et al, 2004;Elnaiem et al, 2005;Meneses et al, 2005;Perrotey et al, 2005;Dvorak et al, 2006Dvorak et al, , 2011Mazzoni et al, 2006Mazzoni et al, , 2008de Souza Rocha et al, 2007;Hamarsheh et al, 2007;Moin-Vaziri et al, 2007a,b;Baron et al, 2008;Bounamous et al, 2008Bounamous et al, , 2014Lins et al, 2008…”

The biogeography and population genetics of neotropical vector species

“…Several allozyme studies (Lanzaro et al, 1993;Dujardin et al, 1997;Mukhopadhyay et al, 1998;Mutebi et al, 2002), a chromosome study (Yin et al, 1998), and mitochondrial DNA sequences (Soto et al, 2001;Arrivillaga et al, 2002Arrivillaga et al, , 2003Hodgkinson et al, 2003) provide evidence for cryptic species except in Brazil, and thus are in disagreement with the pheromone, courtship song, period and cacophony sequence and microsatellite data discussed above.…”