Mitochondrial complex I ROS production and redox signaling in hypoxia

Okoye, Chidozie N.; Koren, Shon A.; Wojtovich, Andrew P.

doi:10.1016/j.redox.2023.102926

Cited by 29 publications

(12 citation statements)

References 313 publications

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“…According to our experiment results, replacement of DMEM (high sugar) with αMEM led to a decrease in ATP production and increase in ROS production. Under hypoxic conditions, the ROS content increases [ 49 ]. In our RNA-seq analysis, we found that PDK1 , LDHA , and SLC7A11 were upregulated in MCF7 cells cultured in αMEM.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-wide analysis of the differences between MCF7 cells cultured in DMEM or αMEM

Jiao,

Zhao,

et al. 2024

PLoS ONE

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MCF7 cells have been used as an experimental model for breast cancer for decades. Typically, a culture medium is designed to supply cells with the nutrients essential for their continuous proliferation. Each medium has a specific nutritional composition. Therefore, cells cultured in different media may exhibit differences in their metabolism. However, only a few studies have investigated the effects of media on cells. In this study, we compared the effects of Dulbecco’s modified Eagle medium (DMEM) and minimum essential medium alpha modification (αMEM) on MCF7 cells. The two media differentially affected the morphology, cell cycle, and proliferation of MCF7 cells, but had no effect on cell death. Replacement of DMEM with αMEM led to a decrease in ATP production and an increase in reactive oxygen species production, but did not affect the cell viability. RNA-sequencing and bioinformatic analyses revealed 721 significantly upregulated and 1247 downregulated genes in cells cultured in αMEM for 48 h compared with that in cells cultured in DMEM. The enriched gene ontology terms were related to mitosis and cell proliferation. Kyoto encyclopedia of genes and genomes analysis revealed cell cycle and DNA replication as the top two significant pathways. MCF7 cells were hypoxic when cultured in αMEM. These results show that the culture medium considerably affects cultured cells. Thus, the stability of the culture system in a study is very important to obtain reliable results.

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Section: Discussionmentioning

confidence: 99%

Transcriptome-wide analysis of the differences between MCF7 cells cultured in DMEM or αMEM

Jiao,

Zhao,

et al. 2024

PLoS ONE

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“…[ROS]: Reactive oxygen species (ROS) encompass a range of oxygen-containing free radicals and peroxides, closely associated with oxygen metabolism in organisms. These typically include peroxides, superoxides, hydroxyl radicals, singlet oxygen, and α-oxygen [ 272 , 273 ]. The detection of ROS primarily relies on the oxidative properties of the substrate.…”

Section: Sensing and Detectionmentioning

confidence: 99%

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Zhang,

Yu,

et al. 2024

Materials Today Bio

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“…Under conditions of hypoxic stress, evidence of ROS formation under conditions of low oxygen content was found [42]. The decrease in the ATP content observed during hypoxia increases the likelihood of ROS formation due to the inhibition of the mitochondrial electron transport chain [43].…”

Section: Content Rosmentioning

confidence: 99%

Features of the Effect of Quercetin on Different Genotypes of Wheat under Hypoxia

Fedoreyeva,

Lazareva,

Kononenko

2024

IJMS

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Hypoxia is one of the common abiotic stresses that negatively affects the development and productivity of agricultural crops. Quercetin is used to protect plants from oxidative stress when exposed to environmental stressors. O2 deficiency leads to impaired development and morphometric parameters in wheat varieties Orenburgskaya 22 (Triticum aestivum L.) and varieties Zolotaya (Triticum durum Desf.). Cytological analysis revealed various types of changes in the cytoplasm under conditions of hypoxia and treatment with quercetin. The most critical changes in the cytoplasm occur in the Zolotaya variety during pretreatment with quercetin followed by hypoxia, and in the Orenburgskaya 22 variety during hypoxia. Quercetin has a protective effect only on the Orenburgskaya 22 variety, and also promotes a more effective recovery after exposure to low O2 content. Hypoxia causes an increase in reactive oxygen species and activates the antioxidant system. It has been shown that the most active components of the antioxidant system in the Orenburgskaya 22 variety are MnSOD and Cu/ZnSOD, and in the Zolotaya variety GSH. We have shown that quercetin provides resistance only to the wheat genotype Orenburgskaya 22, as a protective agent against abiotic stress, which indicates the need for a comprehensive study of the effects of exogenous protectors before use in agriculture.

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Mitochondrial complex I ROS production and redox signaling in hypoxia

Cited by 29 publications

References 313 publications

Transcriptome-wide analysis of the differences between MCF7 cells cultured in DMEM or αMEM

Transcriptome-wide analysis of the differences between MCF7 cells cultured in DMEM or αMEM

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Features of the Effect of Quercetin on Different Genotypes of Wheat under Hypoxia

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