MITOCHONDRIAL BIOGENESIS IN <i>NEUROSPORA CRASSA  </i>

Howell, Neil; Zuiches, Carol A.; Munkres, Kenneth D.

doi:10.1083/jcb.50.3.721

Cited by 33 publications

(3 citation statements)

References 39 publications

(37 reference statements)

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“…Ethanol, at the same Wnal concentration (2% v/v), was used in growth measurement controls. Chloramphenicol is an antibiotic inhibiting mitochondrial protein synthesis, e.g., cytochrome c oxidase and malate dehydrogenase (Howell et al, 1971). Concentrations of 1-4 mg/ml are reported to inhibit mitochondrial protein synthesis in vitro (Sebald et al, 1968) and in vivo (Howell et al, 1971).…”

Section: Treatment With Inhibitorsmentioning

confidence: 99%

The role of tip-localized mitochondria in hyphal growth

Levina¹,

Lew²

2006

Fungal Genetics and Biology

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Section: Treatment With Inhibitorsmentioning

confidence: 99%

The role of tip-localized mitochondria in hyphal growth

Levina¹,

Lew²

2006

Fungal Genetics and Biology

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“…1971). Moreover, cellular changes are observed during growth in the presence of CAP: the structure of the mitochondria is more or less disorganized (Kellerman et ai., 1969;Lenk and Penman, 1971;Howell et al, 1971).…”

Section: Permanent and Long-lasting Effects Of Cap On Morphologymentioning

confidence: 99%

Reversible and permanent effects of the carbon sources and various antibiotics on the morphology and metabolic properties of Ustilago cynodontis cells.

Durieu-Trautmann¹,

Tavlitzki²

1975

The Journal of Cell Biology

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The effects of various carbon sources and of antibiotics on the morphology of hypha cells of the fungus Ustilago cynodontis is described. Nonfermentable substrates promote readily reversible yeastlike colonies from hypha cells: all the hypha cells spread on these substrates give rise to yeastlike colonies that revert to the mycelial phenotype when transferred to glucose medium. Among the antibiotics tested, chloramphenicol (CAP) is found to promote, under certain circumstances, a long-lasting, even permanent modification on the morphology of the colonies: the colonies developed on CAP-glucose media are yeastlike, and a percentage of them give rise to colonies whose morphology remains yeastlike even on drug-free media; this effect is also obtained with cells cultivated in liquid medium. This permanent morphological modification is accompanied by a change of metabolic properties. Similar permanent effects are obtained with ethidium bromide, suggesting that mitochondrial functioning is involved in these modifications.Ustilago cynodontis, an obligate aerobic fungus, has two morphologically different cell types: mycelial and yeastlike which, on solid 4% glucose medium, give rise to colonies that are morphologically different also (Fig. 1). Yeastlike strains are derived from mycelial cells (Nozeran and Chevalier, 1965) and it has been shown that mycelial cells of the 4001 strain (M1) are prototrophic whereas yeastlike cells (L1) which are derived from this M1 mycelial strain require lysine and arginine to grow (Talou and Tavlitzki, 1969),In the present paper, reversible changes and long-lasting, even permanent changes in the morphology of mycelial cells and colonies of the 4001 strain are studied.It is observed that as in many other fungi the morphology of the colonies obtained from mycelial hyphae is dependent on the nature of the carbon source in the growth medium: yeastlike development is promoted on media containing a nonfermentable substrate as the carbon source, but this state is reversible, that is, the colonies remain yeastlike as long as the nonfermentable substrate is present in the growth medium and the phenotypic change reverts to the mycelial form when the cells are transferred to a fermentable growth medium.On the other hand, the results reported in this paper show that chloramphenicol (CAP) and ethidium bromide (EB) can initiate a long-lasting or permanent modification of the mycelial cells,

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“…Filamentous fungi are regarded as obligate aerobes, although a small number can produce ethanol from sugars under anaerobic conditions. For example, Fusarium lini and other Fusarium species as well as Neurospora crassa were found t o produce ethanol from glucose and/or sucrose (Cochrane 1956;Heath et al 1956;Howell et al 1971;Suikho & Enari 1981). A wide variety of sugars and starch were fermented by a n unidentified Paecilomyces species to give ethanol yields of 1 4 % (Wu et al 1986).…”

Section: Discussionmentioning

confidence: 99%

Ethanol production in table jelly by two species of Chrysosporium

Kinderlerer

1987

Journal of Applied Bacteriology

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Different commercial brands of tablet jelly were stored in sealed containers for 12 weeks at an initial water activity of 0.90. The surface of those containing real fruit juice became covered with a dense white mould identified as Chrysosporium inops. That of those without fruit juice did not. This type of spoilage has been observed in commercially stored samples. When spores of this fungus and the closely related species, C. xerophilum, were added to commercial tablet jelly, the fungi grew only on samples containing real fruit juice. It is suggested that the fruit juice may provide some essential nutrient required for growth of these nutritionally fastidious species as well as the fungal spores themselves. Ethanol was detected in the headspace gas of stored samples of mouldy jelly. The production of ethanol by the two Chrysosporium species was confirmed by fermentation of glucose in liquid batch culture: approximately 2% ethanol was formed in 14 d. The ability of C. xerophilum and C. inops to utilize the Embden-Meyerhof-Parnas pathway may explain why these slow-growing xerophilic moulds become dominant after storage at low oxygen tension.

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MITOCHONDRIAL BIOGENESIS IN NEUROSPORA CRASSA

Cited by 33 publications

References 39 publications

The role of tip-localized mitochondria in hyphal growth

The role of tip-localized mitochondria in hyphal growth

Reversible and permanent effects of the carbon sources and various antibiotics on the morphology and metabolic properties of Ustilago cynodontis cells.

Ethanol production in table jelly by two species of Chrysosporium

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