Mitochondrial and glycolytic activity of UV-irradiated human keratinocytes and its stimulation by a Saccharomyces cerevisiae autolysate

Schütz, Rolf; Kuratli, Karin; Richard, Nathalie; Stoll, Clarissa; Schwager, Joseph

doi:10.1016/j.jphotobiol.2016.03.031

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…Finally, our results suggest that the clear association of the m 1 A 947 16S rRNA modification with mtDNA transcript levels in skin not exposed to sun was lost in sun-exposed skin. Previously, it was shown that UV irradiation changes mitochondrial morphology and reduces mitochondrial oxygen consumption rate in skin keratinocytes, as well as altering expression of mitochondria-related genes (Leung et al 2013;Schutz et al 2016;Kelly and Murphy 2018). Accordingly, it is conceivable that the impact of m 1 A 947 16S rRNA modification on mtDNA transcript levels is influenced by UV exposure.…”

Section: Discussionmentioning

confidence: 99%

mtDNA eQTLs and the m1A 16S rRNA modification explain mtDNA tissue-specific gene expression pattern in humans

Cohen

Mordechai

Eran

et al. 2018

Preprint

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Expression quantitative trait loci (eQTLs) are instrumental in genome-wide identification of regulatory elements, yet were overlooked in the mitochondrial DNA (mtDNA). By analyzing 5079 RNA-seq samples from 23 tissues we identified association of ancient mtDNA SNPs (haplogroups T2, L2, J2 and V) and recurrent SNPs (mtDNA positions 263, 750, 1438 and 10398) with tissue-dependent mtDNA gene-expression. Since the recurrent SNPs independently occurred in different mtDNA genetic backgrounds, they constitute the best candidates to be causal eQTLs. Secondly, the discovery of mtDNA eQTLs in both coding and non-coding mtDNA regions, propose the identification of novel mtDNA regulatory elements.Third, we identified association between low m 1 A 947 MT-RNR2 (16S) rRNA modification levels and altered mtDNA gene-expression in twelve tissues. Such association disappeared in skin which was exposed to sun, as compared to sun-unexposed skin from the same individuals, thus supporting the impact of UV on mtDNA gene expression. Taken together, our findings reveal that both mtDNA SNPs and mt-rRNA modification affect mtDNA gene expression in a tissue-dependent manner.

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Section: Discussionmentioning

confidence: 99%

mtDNA eQTLs and the m1A 16S rRNA modification explain mtDNA tissue-specific gene expression pattern in humans

Cohen

Mordechai

Eran

et al. 2018

Preprint

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“…A similar trend was observed in another recent study that found an increase in SIRT4 at 72 h after UVR (30 mJ cm À2 of 75% UVB and 25% UVA) (34). Although there is currently no direct evidence linking SIRT5 to photoaging or UVR, it appears that SIRT5 may contribute to the retrieval of cell viability after UVR in keratinocytes due to its role in glycolysis regulation (35), as the augmentation of glycolysis can recover UV-stressed keratinocytes (60 mJ cm À2 of 68% UVB and 32% of UVA) (36). Overall, the available data suggest that mitochondrial sirtuins, especially SIRT4 may play a key role in the response of human keratinocytes to UV damage, and may, therefore, be potential targets to combat photoaging.…”

Section: Role In Ultraviolet Radiation (Uvr) Response and Photoagingmentioning

confidence: 99%

Mitochondrial Sirtuins in Skin and Skin Cancers

Ndiaye

Singh

et al. 2020

Photochem & Photobiology

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Mammalian sirtuins (SIRTs 1‐7) are a family of NAD+‐dependent deacetylases with distinct subcellular localization and biological functions that regulate various important cellular processes. Among these, SIRTs ‐3, ‐4 and ‐5 are located in the mitochondria and have been implicated in caloric restriction, oxidative stress, aging and various human diseases. Emerging evidence has found dysregulation of mitochondrial sirtuins in multiple dermatological conditions, including responses to ultraviolet radiation (UVR), suggesting their importance in maintaining skin health. In this review, we discuss the roles and implications of mitochondrial sirtuins in cutaneous cellular processes, and their emerging potential as a target for the management of skin diseases, including skin cancer. Among mitochondrial sirtuins, SIRT3 is the most studied and linked to multiple skin conditions and diseases (keratinocyte differentiation, wound healing, chronological aging, UVR and ozone response, systemic sclerosis, melanoma, basal cell carcinoma (BCC) and squamous cell carcinoma (SCC)). SIRT4 has been connected to keratinocyte differentiation, chronological aging, UVR response, alopecia, BCC and SCC. Further, SIRT5 has been associated with keratinocyte differentiation, melanoma, BCC and SCC. Overall, while there is compelling evidence for the involvement of mitochondrial sirtuins in skin, additional detailed studies are needed to understand their exact roles in skin and skin cancers.

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“…The aging phenomenon is a multifactorial process in which a high energy metabolism is essential to maintain cellular homeostasis, repair damage, and slow down the process of cellular deterioration. Thus, the decline in energy metabolism caused by DNA-damaging agents accelerates the aging process and, conversely, high glycolytic and respiratory activity increase the cellular resistance to environmental agents that cause stress [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Resistance to Ionizing Radiation in S. cerevisiae and Its Relationship with Aging, Oxidative Stress, and Antioxidant Activity

González-Vidal,

Mercado-Sáenz,

Burgos-Molina

et al. 2023

Antioxidants

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The repair of the damage produced to the genome and proteome by the action of ionizing radiation, oxidizing agents, and during aging is important to maintain cellular homeostasis. Many of the metabolic pathways influence multiple processes. In this way, this work aims to study the relationship between resistance/response to ionizing radiation, cellular aging, and the response mechanisms to oxidative stress, free radicals, reactive oxygen species (ROS), and antioxidant activity in the yeast S. cerevisiae. Systems biology allows us to use tools that reveal the molecular mechanisms common to different cellular response phenomena. The results found indicate that homologous recombination, non-homologous end joining, and base excision repair pathways are the most important common processes necessary to maintain cellular homeostasis. The metabolic routes of longevity regulation are those that jointly contribute to the three phenomena studied. This study proposes eleven common biomarkers for response/resistance to ionizing radiation and aging (EXO1, MEC1, MRE11, RAD27, RAD50, RAD51, RAD52, RAD55, RAD9, SGS1, YKU70) and two biomarkers for response/resistance to radiation and oxidative stress, free radicals, ROS, and antioxidant activity (NTG1, OGG1). In addition, it is important to highlight that the HSP104 protein could be a good biomarker common to the three phenomena studied.

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Mitochondrial and glycolytic activity of UV-irradiated human keratinocytes and its stimulation by a Saccharomyces cerevisiae autolysate

Cited by 10 publications

References 25 publications

mtDNA eQTLs and the m1A 16S rRNA modification explain mtDNA tissue-specific gene expression pattern in humans

mtDNA eQTLs and the m1A 16S rRNA modification explain mtDNA tissue-specific gene expression pattern in humans

Mitochondrial Sirtuins in Skin and Skin Cancers

Molecular Mechanisms of Resistance to Ionizing Radiation in S. cerevisiae and Its Relationship with Aging, Oxidative Stress, and Antioxidant Activity

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