Mitochondrial alternative oxidase (AOX1a) is required for the mitigation of arsenic-induced oxidative stress in Arabidopsis thaliana

Demircan, Nil; Cucun, Gokhan; Uzilday, Barış

doi:10.1007/s11816-020-00595-9

Cited by 20 publications

(11 citation statements)

References 42 publications

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“…In previous studies, it was noticed that catalase activity in the seedlings decreased under stress and at the same time oxidase activity was increased. Similarly, in our studies, it is elucidated that the time in which seedlings were in stress, the catalase activity was low while the oxidase activity was high (Demircan et al, 2020).…”

Section: Discussionsupporting

confidence: 76%

Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid

Yousaf

Hussain

Iqbal

et al. 2021

Front. Bioeng. Biotechnol.

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Auxin is the reciprocal signaling molecule, which interferes with other phyto-hormonal and physiological processes during plant–microbes interaction. In this regard, Bipolaris spp., a growth-promoting endophytic fungus was used to inoculate pre-stressed Zea mays seedlings with yucasin (IAA inhibitor). The IAA-deficient host was heavily colonized by the endophyte that subsequently promoted the host growth and elevated the IAA levels with a peak value at 72 h. However, the seedling growth was inhibited later (i.e., at 120 h) due to the high levels of IAA that interfered with the activity of phytoalexins and brassinosteroids. Such interference also modulated the endophytic fungus from symbiotic to biotrophic pathogen that left the host plants defenseless.

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Section: Discussionsupporting

confidence: 76%

Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid

Yousaf

Hussain

Iqbal

et al. 2021

Front. Bioeng. Biotechnol.

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“…1e-g). Increased formation of ROS is a common effect of As toxicity in cells as a result of a disturbance in redox homeostasis, leading to an over-reduction of mitochondrial and chloroplastic electron transport chains and impaired metabolism, as well as by induction of ROS-dependent signaling pathways (Rodríguez-Ruiz et al 2019;Demircan et al 2020). Exogenous application of JA in As-stressed plants was able to modify the pattern of ROS accumulation (Fig.…”

Section: Ja Partially Reverts Ros Accumulation and Membrane Damagementioning

confidence: 95%

Exogenous jasmonic acid enhances oxidative protection of Lemna valdiviana subjected to arsenic

et al. 2020

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Oxidative damage is one of the most harmful effects arising from arsenic (As) toxicity in plants. Herein, the role of exogenous jasmonic acid (JA) in the modulation of As-induced oxidative stress in Lemna valdiviana was investigated. Plants were grown for 24 h in Clark's nutritive solution containing As (4.0 mg L −1) or As + JA (50, 100, 250 and 500 µM). Chlorophyll a and b content decreased under As stress, either in isolation or associated with JA. The decreased chlorophyll a/b ratio in As-exposed plants was recovered by JA treatment at 100 µM. The carotenoid content was higher in plants exposed to As compared to controls and lower when it was associated with JA. Arsenic triggered the accumulation of O 2 •− and H 2 O 2 , in addition to severely increasing lipid peroxidation. Application of JA in As-grown plants resulted in lower O 2 •− content and lipid peroxidation than in those grown under As alone, as a result of enhanced SOD activity. On the other hand, H 2 O 2 accumulation was increased by JA in As-stressed plants, associated with higher CAT, POX and GPX activity. The As content and bioaccumulation factor (BF) were improved by application of JA in the nutritive solution at 250 and 500 µM. Our findings indicate that JA modulates the pigment balance, thereby fine-tuning energy dissipation as well as alleviating As-induced oxidative damage in L. valdiviana through modulation of ROS homeostasis and improvement of the antioxidant enzymatic system, allowing increased accumulation of As without showing major damage.

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“…Do these AOX isoforms share the same functionality as AOX1a under certain perturbed metabolic conditions or do they act independently? While the upregulation of AOX1d transcripts during senescence (Clifton et al, 2006) and various stressful treatments (Strodtkotter et al, 2009, Feng et al, 2013, Demircan et al, 2020 is known, the perceived low abundance of AOX1d, compared to AOX1a, has limited further investigation (Fig 1C). In this study, we provided genetic and biochemical evidence of AOX1d's role in Pro catabolism and its apparent functional redundancy to AOX1a in this role.…”

Section: Both Aox1a and Aox1d Are Responsible For Aox Capacity In Metc Upon Pro Exposurementioning

confidence: 99%

“…Both AOX1a and AOX1d demonstrate a clear pattern of elevated expression under a range stress conditions such as salinity (Van Aken et al, 2009, Feng et al, 2013 and low nitrogen stress (Watanabe et al, 2010) and also in response to chemical inhibition of mETC complexes, such as with antimycin A (Strodtkotter et al, 2009). Manipulation of AOX1a expression in Arabidopsis under diverse environmental treatments such as cold (Fiorani et al, 2005), drought (Giraud et al, 2008), high CO 2 concentrations (Gandin et al, 2012), high arsenic (Demircan et al, 2020) and ecologically relevant doses of UV-B (Garmash et al, 2020) have highlighted the potential of AOX1a to enable acclimation to unfavourable growth conditions. AOX1a expression is particularly strongly upregulated by chemical disruption of complex II and III, and has been linked to mitochondrial retrograde signalling (Sweetlove et al, 2002, Clifton et al, 2005, Schwarzländer et al, 2011, Umbach et al, 2012 and the alleviation of excess reducing power arising from impediments to normal mETC activity (Jiang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Alternative oxidase1a and 1d limit proline-dependent oxidative stress and aid salinity recovery in Arabidopsis

O’Leary

Signorelli

et al. 2021

Preprint

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A link between Pro catabolism and mitochondrial reactive oxygen species production has been established across eukaryotes and in plants increases in leaf respiration rates have been reported following Pro exposure. Here we investigated how alternative oxidases (AOXs) of the mitochondrial electron transport chain accommodate the large, atypical flux resulting from Pro catabolism and limit oxidative stress during Pro breakdown in mature Arabidopsis leaves. Following Pro treatment, AOX1a and AOX1d accumulate at transcript and protein levels, with AOX1d approaching the level of the typically dominant AOX1a isoform. We therefore sought to determine the function of both AOX isoforms under Pro respiring conditions. Oxygen consumption rate measurements in aox1a and aox1d leaves suggested these AOXs can functionally compensate for each other to establish enhanced AOX catalytic capacity in response to Pro. Generation of aox1a.aox1d lines showed complete loss of AOX proteins and activity upon Pro treatment, yet respiratory induction in response to Pro was still possible via the cytochrome pathway. However, aox1a.aox1d leaves suffered increased levels of oxidative stress and damage during Pro metabolism compared to WT or the single mutants. During recovery from salt stress, when high rates of Pro catabolism occur naturally, photosynthetic rates in aox1a.aox1d recovered slower than WT or the single aox lines, showing that both AOX1a and AOX1d are beneficial for cellular metabolism during Pro drawdown following osmotic stress. This work provides physiological evidence of a beneficial role for AOX1a but also the less studied AOX1d isoform in allowing safe catabolism of alternative respiratory substrates like Pro.

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Mitochondrial alternative oxidase (AOX1a) is required for the mitigation of arsenic-induced oxidative stress in Arabidopsis thaliana

Cited by 20 publications

References 42 publications

Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid

Transformation of Endophytic Bipolaris spp. Into Biotrophic Pathogen Under Auxin Cross-Talk With Brassinosteroids and Abscisic Acid

Exogenous jasmonic acid enhances oxidative protection of Lemna valdiviana subjected to arsenic

Alternative oxidase1a and 1d limit proline-dependent oxidative stress and aid salinity recovery in Arabidopsis

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