Mitochondrial A10398G Alteration in Plasma Exosome of Non-small Cell Lung Cancer Patients

Abstract

This study identifies A10398G alteration of mitochondrial ND3 gene in plasma exosome of 29 non-small cell lung cancer (NSCLC) patients, 31 controls and 13 pairs of tumor tissue and adjacent tissue of NSCLC patients, thereby assessing the relationship between this alteration in plasma exosome and tissue as well as the pathological characteristics of NSCLC patients. Using the PCR-RFLP method, the homoplasmy and heteroplasmy of A10398G were initially identified in mitochondrial DNA from both exosomes and lung tissues. The rate of variant 10398G in plasma exosome was 62.1% in the NSCLC group and 61.3% in the control group. However, there was no statistically significant difference in A10398G between the patient and control groups. The alteration of A10398G in plasma exosome and in tissue correlated with each other (correlation coefficient 0.69; p = 0.009). However, this alteration was not related to age, gender, smoking, alcohol drinks status, tumor size, histological stage and TNM stage.
 Keywords
 A10398G alteration, mitochondrial DNA, plasma exosome, non-small cell lung cancer.
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Impact of Storage Conditions on EV Integrity/Surface Markers and Cargos

“…The following studies also confirmed that all eukaryotes and prokaryotes could produce EVs, and it was considered that cells used these EVs as cargo transporters to transfer unwanted materials outwards [ 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. However, recent research has shown that EVs are crucial for intercellular communication throughout normal and pathological development [ 11 , 12 , 13 , 14 , 15 ]; in fact, some of them are involved in cancer progression [ 16 , 17 , 18 , 19 ], obesity and metabolic diseases [ 20 , 21 , 22 , 23 , 24 ], inflammatory and autoimmune pathogenesis [ 25 , 26 , 27 ]. Nowadays, various studies revealed that EVs are used in several clinical applications such as disease diagnosis and development of vaccines, drug distribution, and extracellular vesicle (EV)-based therapies [ 17 , 18 , 19 ].…”