Mitochondria-targeted fluorescence probe for endogenous hypochlorite imaging in living cells and zebrafishs

Ji, Dandan; Li, Gang; Zhang, Shuman; Zhu, Mengyao; Li, Chao; Qiao, Renzhong

doi:10.1016/j.snb.2017.12.089

Cited by 38 publications

(16 citation statements)

References 27 publications

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“…This enabled the ratiometric imaging of endogenous ClO − stimulated by lipopolysaccharide (LPS) and was shown to localize in the mitochondria of RAW264.7 cells. Other mitochondria‐targeting probes have been developed based on BODIPY modified with a phenolic hydroxyl protecting group. The probes achieved the sensitive imaging of the basal ClO − concentration and fluctuations of endogenous/exogenous ClO − in the mitochondria of HeLa cells, as well as in zebra fish embryos ( Figure ) .…”

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

“…Other mitochondria‐targeting probes have been developed based on BODIPY modified with a phenolic hydroxyl protecting group. The probes achieved the sensitive imaging of the basal ClO − concentration and fluctuations of endogenous/exogenous ClO − in the mitochondria of HeLa cells, as well as in zebra fish embryos ( Figure ) . A water‐soluble and pH‐independent probe was also reported by Guo and co‐workers based on rhodol fluorophore for the imaging in A549 cells and in mice.…”

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Section: Fluorescent Imaging Agents For Peroxynitritementioning

confidence: 99%

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Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

et al. 2019

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Reactive oxygen species (ROS) consist of a diverse range of oxidative small molecular ions and free radicals that are produced throughout the body during certain biological processes. Due to their high reactivity, these molecules result in the damage of tissues and cells. Therefore, ROS have been implicated in an array of diseases including cancer, inflammation, and neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Owing to the simplicity, sensitivity, and selectivity of fluorescence‐based strategies, many small‐molecular sensors or imaging agents have been developed to sense and visualize ROS both in vitro and in vivo. Likewise, activatable drug delivery and prodrug systems that can be triggered by ROS for disease theranostics (diagnostic and therapeutic combined) have been developed. Herein, recently developed fluorescence‐based sensing and imaging agents for the detection of ROS both intracellularly and in vivo are summarized. In addition, drug delivery, which require activation by ROS to achieve disease theranostics is also discussed.

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Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Section: Fluorescent Imaging Agents For Peroxynitritementioning

confidence: 99%

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Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

et al. 2019

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“…ROS (reactive oxygen species) are generated in the normal physiological metabolism of cells, which play a crucial role in biological metabolism . As one of the most important ROS in living system, Hypochlorous/Hypochlorite (HClO/ClO − ) takes a critical role in many kinds of physiological processes and can be endogenously formed in activated leukocytes by the reaction of chloride ions and hydrogen peroxide with heme‐containing enzyme myeloperoxidase (MPO) served as the catalyst .…”

Section: Introductionmentioning

confidence: 99%

A Fluorescent Probe with a Significant Selective Turn‐On Response for HClO Detection and Bioimaging in Living Cells

et al. 2019

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Hypochlorous/Hypochlorite(HClO/ClO À ) plays an important biochemistry roles in normal operation of the life system. Herein, a fluorescent probe 7-chloro-3-((E)-3-(4-(dimethylamino)phenyl)acryloyl)-2H-chromen-2-one (CCO) was rationally designed and synthesized via aldehyde ketone condensation reaction to detect ClO À in Tris-HCl (pH = 7.2, 60% DMF) solution. Probe CCO was cleaved conceivably in the coumarin lactone ring by an oxidation reaction with ClO À and emitted intensive bright yellow fluorescence. The sensing mechanism was further confirmed by ESI-MS and DFT calculation. The fluorescence emission of probe CCO triggered by ClO À was obviously enhanced within 20 s. The limit of detection of probe CCO was calculated as low as 2.6 nM. Furthermore, probe CCO can be given as an effective and outstanding fluorescent exogenous ClO À probe in living HEK293T cells for microscopic cell imaging.

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Mitochondria‐Targeting BODIPY Probes for Imaging of Reactive Oxygen Species

Sun

Tang

et al. 2023

Advanced Sensor Research

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Reactive oxygen species (ROSs) are an important class of signaling molecules that play a critical role in regulating physiological processes in the human body. Mitochondria are the primary site of ROSs production, and abnormal concentrations of ROSs can lead to the malignant proliferation of cells, resulting in diseases. Therefore, it is crucial to detect ROSs in mitochondria. Fluorometric methods have gained significant attention from scientists because of their ease of observation, simplicity of operation, and noninvasiveness. Among the fluorescent dyes, boron‐dipyrromethene (BODIPY) stands out for its high quantum yield, large molar extinction coefficient, and excellent chemical stability, making it widely used in fluorescent imaging. The common design principle of fluorescent probe for the detection of ROSs includes photo‐induced electron transfer, intramolecular charge transfer, and fluorescence resonance energy transfer. This paper provides an overview of BODIPY‐based fluorescent probes designed for imaging ROSs in mitochondria, covering the sensing mechanisms, molecular engineering strategies, and recent advancements. Additionally, the review provides insight into the potential clinical applications of mitochondria‐targeting BODIPY probes in disease diagnosis.

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Mitochondria-targeted fluorescence probe for endogenous hypochlorite imaging in living cells and zebrafishs

Cited by 38 publications

References 27 publications

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

A Fluorescent Probe with a Significant Selective Turn‐On Response for HClO Detection and Bioimaging in Living Cells

Mitochondria‐Targeting BODIPY Probes for Imaging of Reactive Oxygen Species

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