Mitochondria-rich (MR) cells and the activities of the and carbonic anhydrase in the gill and opercular epithelium of Oreochromis mossambicus adapted to various salinities

Laser scanning cytometry and tissue microarray analysis of salinity effects on killifish chloride cells

“…Our data support the notion that CC hypertrophy is much more closely correlated with salt secretory capacity than CC number (Foskett et al, 1981). Size distribution profiles of CC obtained in this study are very similar to those obtained previously in O. mossambicus (Kültz et al, 1992). In FW-adapted fish there is only one population of small mitochondria-rich cells (FW CC) while in SW there are two populations.…”

“…Thus, in F. heteroclitus salinity-dependent CC proliferation may be regulated differently in gill and opercular epithelia. Such differential regulation of CC proliferation in gill and opercular epithelia contrasts to other euryhaline teleosts, for instance Oreochromis mossambicus, where CC number increases in both types of epithelia proportional to external salinity (Kültz et al, 1992). Our data support the notion that CC hypertrophy is much more closely correlated with salt secretory capacity than CC number (Foskett et al, 1981).…”

“…Although it appears that most natural environments are characterized by gradual changes in salinity we know much more about CC responses to acute salinity transfer (e.g. Kültz et al, 1992). Interestingly, CC number in opercular epithelium in F. heteroclitus does not change in response to acute transfer from FW to 1× SW (Daborn et al, 2001).…”

Prolonged apoptosis in mitochondria-rich cells of tilapia (Oreochromis mossambicus) exposed to elevated salinity

“…At the same time, this antibody enabled us to quantify NKA abundance in individual MRC and to determine MRC size. While NKA antibodies have previously been used as a MRC marker (Lima and Kültz 2004;Sardella et al 2008) and MRC size has often been measured after live DAPSMI/DASPEI staining (Foskett et al 1981;Kültz et al 1992;Van Der Heijden et al 1997), the Wxation of whole cells on PLL-coated coverslips allowed us to combine these two approaches and utilize NKA antibodies for determination of MRC size in Wxed, dissociated cell populations. This method is advantageous over live cell stains such as DASPMI when co-labeling with dyes that require Wxed cells is desired (for instance TUNEL labeling) and also to measure NKA abundance per MRC.…”

Osmolality/salinity-responsive enhancers (OSREs) control induction of osmoprotective genes in euryhaline fish

“…The osmosensory signaling networks that control these effector genes are most potent and apparent in euryhaline species with highly dynamic osmoregulatory ability (8). One such species is Oreochromis mossambicus, which has a very wide salinity tolerance range of 0-120 g/kg (9) and is a well-established model for studies of teleost osmoregulation (10)(11)(12).…”