Mitochondria-rich cells in anuran Amphibia: chloride conductance and regional distribution over the body surface

Katz, Uri; Rozman, Alexander; G, Zaccone; Fasulo, Salvatore; Gabbay, S.

doi:10.1016/s1095-6433(99)00168-3

Cited by 17 publications

(4 citation statements)

References 26 publications

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“…MRCs showed a strong affinity for UEA‐I lectin in the epidermis of the thumb pad of P. ridibundus . Although these cells did not show any affinity for WGA, DBA and PNA in the present study, they showed an affinity for WGA and PNA lectin in B. viridis , and they showed an affinity for solely PNA lectin in B. marinus (Zaccone et al, ; Katz et al, ). The presence of MRC in dorsal and ventral amphibian skin, and its role in relation to osmoregulation, is well established (Martinez‐Palamo et al, ; Helman and Fisher, ; Ehrenfeld and Klein, ).…”

Section: Discussionmentioning

confidence: 52%

Seasonal lectin binding variations of thumb pad in the frog (Pelophylax ridibundus)

Kaptan

Bolkent

2013

Journal of Morphology

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The thumb pad is one of the most common secondary sexual characteristics in frogs. Although it is known that amphibian skin has affinity for several lectins, there is no report regarding lectin-binding affinity of the thumb pad or its structural components. This study investigated localization and seasonal variation of specific carbohydrate moieties of glycoconjugates in both the epidermal and dermal components of the frog thumb pad at the light microscopic level using lectin histochemistry. The study consisted of four seasonal groups of the frog species, Pelophylax ridibundus (Synonym of Rana ridibunda): active, prehibernating, hibernating and posthibernating. Four horseradish peroxidase conjugated lectins were employed. It was found that dolichos biflorus agglutinin (DBA), wheat germ agglutinin (WGA), and ulex europaeus (UEAI) gave positive reactions in both epidermal layers and breeding glands. These three lectins bound specific secretory cells in the breeding glands, and the distribution of the cells and epithelial lectin reactions exhibited seasonal changes. In addition, UEA-I and peanut agglutinin (PNA) showed an affinity in granular glands and the granular zone of mixed glands. Generally, epidermal lectin binding showed dense affinity during the posthibernation period. DBA, UEA-I, and WGA-specific cells in the mucous gland decreased gradually until the posthibernation period. These findings suggest that differences of lectin binding in the thumb pad may be related to functional activities and, thus, seasonal adaptations. Moreover, the presence of specific lectin-binding cells in the breeding glands indicated that they consisted of heterogeneous secretory cell composition or that the cells were at different secretory stages.

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Section: Discussionmentioning

confidence: 52%

Seasonal lectin binding variations of thumb pad in the frog (Pelophylax ridibundus)

Kaptan

Bolkent

2013

Journal of Morphology

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“…As is the case for the above‐mentioned concentration dependence of P Cl , the voltage dependence also seems to be a general feature of anurans: R. temporaria and Rana esculenta (Kristensen 1983), Bufo viridis (Katz & Larsen 1984), Bufo marinus (Larsen et al. 1987, Lacaz‐Vieira & Procopio 1988) and Hyla arborea (Katz et al. 2000).…”

Section: Voltage Dependence Of the Passive Chloride Permeabilitymentioning

confidence: 71%

Reconciling the Krogh and Ussing interpretations of epithelial chloride transport – presenting a novel hypothesis for the physiological significance of the passive cellular chloride uptake

Larsen

2011

Acta Physiologica

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In 1937, August Krogh discovered a powerful active Cl(-) uptake mechanism in frog skin. After WWII, Hans Ussing continued the studies on the isolated skin and discovered the passive nature of the chloride uptake. The review concludes that the two modes of transport are associated with a minority cell type denoted as the γ-type mitochondria-rich (MR) cell, which is highly specialized for epithelial Cl(-) uptake whether the frog is in the pond of low [NaCl] or the skin is isolated and studied by Ussing chamber technique. One type of apical Cl(-) channels of the γ-MR cell is activated by binding of Cl(-) to an external binding site and by membrane depolarization. This results in a tight coupling of the uptake of Na(+) by principal cells and Cl(-) by MR cells. Another type of Cl(-) channels (probably CFTR) is involved in isotonic fluid uptake. It is suggested that the Cl(-) channels serve passive uptake of Cl(-) from the thin epidermal film of fluid produced by mucosal glands. The hypothesis is evaluated by discussing the turnover of water and ions of the epidermal surface fluid under terrestrial conditions. The apical Cl(-) channels close when the electrodiffusion force is outwardly directed as it is when the animal is in the pond. With the passive fluxes eliminated, the Cl(-) flux is governed by active transport and evidence is discussed that this is brought about by an exchange of cellular HCO(3) (-) with Cl(-) of the outside bath driven by an apical H(+) V-ATPase.

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“…Three ENaC subtypes have been reported in PCs and MRCs in the larvae and adult skin of the Japanese tree frog (Takada et al, 2006). Furthermore, MRCs have been divided into three subtypes (Larsen et al, 1987; Hillyard et al, 2009), which are different from PCs, according to immunohistochemical observations (Zaccone et al, 1986; Katz et al, 2000). The α‐type MRC is specialized for H + secretion, whereas the β‐type MRC is specialized for eliminating a base surplus as HCO

_{3}^{−}

and is associated with Cl − uptake.…”

Section: Discussionmentioning

confidence: 99%

Immunohistological classification of ionocytes in the external gills of larval Japanese black salamander, Hynobius nigrescens Stejneger

Uchiyama¹,

Kumano²,

Komiyama³

et al. 2011

Journal of Morphology

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In this cytological and immunohistological study, we clarified the localization of the membrane transporters Na(+) , K(+) -ATPase (NKA), vacuolar-type H(+) -ATPase (VHA), and epithelial sodium channel (ENaC) and distinguished ionocyte subtypes in the gill of the Japanese salamander (Hynobius nigrescens). In larvae (IY stages 43-65), NKA immunoreactivity was observed on the basolateral plasma membrane in more than 60% cells and less than 20% cells in the primary filaments and secondary lamellae of the external gills, respectively. VHA immunoreactivity was observed on the apical membrane of some epithelial cells in the secondary lamellae of the external gills. High ENaCα immunoreactivity was widely observed on the apical cell membrane of a population of squamous cells, presumably pavement cells (PVCs), and mitochondria-rich cells (MRCs), in the primary filaments and secondary lamellae of the external gills. Using double immunofluorescence microscopy, epithelial cell types involved in ionic regulation were characterized and divided into three ionocyte types: NKA-, NKA- and ENaC-, and VHA-positive cells. VHA-immunoreactive cells as well as NKA-positive cells were observed during IY stages 43-65 of the salamander larvae. During late stages of metamorphosis, NKA, VHA, and ENaCα immunoreactivities in the external gills decreased and finally disappeared during the completion of metamorphosis (IY stage 68). PVCs and MRCs in the external gills are probably involved in acid-base balance regulation and osmoregulation in urodele amphibian larvae. The results are discussed in relation to the ionocytes previously reported in fish gills and the frog skin epithelium.

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Mitochondria-rich cells in anuran Amphibia: chloride conductance and regional distribution over the body surface

Cited by 17 publications

References 26 publications

Seasonal lectin binding variations of thumb pad in the frog (Pelophylax ridibundus)

Seasonal lectin binding variations of thumb pad in the frog (Pelophylax ridibundus)

Reconciling the Krogh and Ussing interpretations of epithelial chloride transport – presenting a novel hypothesis for the physiological significance of the passive cellular chloride uptake

Immunohistological classification of ionocytes in the external gills of larval Japanese black salamander, Hynobius nigrescens Stejneger

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