Mitochondria-rich cells and carbonic anhydrase content of toad skin epithelium

Katz, Uri; Gabbay, S.

doi:10.1007/bf00215851

Cited by 28 publications

(5 citation statements)

References 22 publications

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“…None of the MR cells, either in the tadpole epidermis or in the adult epithelium showed any reaction with PAS. The MR cells in the epithelium of the adult are stained by silver (unpublished observation), resembling MR cells in other anurans [9,18]. The rarity of MR cells in the tadpole epidermis does not permit a definitive conclusion as to whether the tadpole's MR cells are stained by silver.…”

Section: Resultsmentioning

confidence: 94%

Developmental changes in the heterocellular epidermis of Pelobates syriacus integument

Gabbay

Rosenberg

Warburg

et al. 1992

Biology of the Cell

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Changes in characteristic components of the skin epidermis of the large tadpole of Pelobates syriacus were studied throughout its development. The fate of two specific cells in the skin epidermis was followed, from the young tadpole to the adult was studied. It was found that flask-shaped type cells in the tadpole epidermis which are PAS-positive, stain with peanut lectin (PNA). There is no detectable band 3 in the premetamorphosed stages, and mitochondria-rich cells are very rare. This pattern of staining changes completely upon metamorphosis: the PAS-positive cells, specific to the tadpole epidermis disappear, and the mitochondria-rich (MR) cells in the adult skin epithelium react with polyclonal anti-band 3 antibody. Western blot analysis showed the presence of a band 3-like protein of about 95 kDa, only in the adult epithelial extract, corroborating the immunocytochemical observations. The finding of the presence of band 3-like protein in the MR cells of Pelobates, is similar to the observations made in the skin of other amphibian species. On the other hand, the binding of peanut lectin to MR cells is species-specific, since it does not react with the MR cells in the skin epithelium of Pelobates syriacus.

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Section: Resultsmentioning

confidence: 94%

Developmental changes in the heterocellular epidermis of Pelobates syriacus integument

Gabbay

Rosenberg

Warburg

et al. 1992

Biology of the Cell

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“…Much attention has been paid to the MR cells of amphibian skin because they provide a useful model for studying ion-transporting mechanisms. The MR cells of amphibian skin con-tain carbonic anhydrase II, which catalyzes the dehydration of CO2 to produce H + and HCO3 - (Rosen and Friedley, 1973;Katz and Gabbay, 1988). HCO3 -is secreted across the skin surface via a Cl -/HCO3 -anion exchanger (band 3) (Jensen et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression of Vacuolar Proton-Pumping ATPase Subunits in the Follicular Epithelium of the Bullfrog Endolymphatic Sac

et al. 2007

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In an investigation aimed at clarifying the mechanism of crystal dissolution of the calcium carbonate lattice in otoconia (the mineral particles embedded in the otolithic membrane) of the endolymphatic sac (ELS) of the bullfrog, cDNAs encoding the A-and E-subunits of bullfrog vacuolar protonpumping ATPase (V-ATPase) were cloned and sequenced. The cDNA of the A-subunit consisted of an 11-bp 5'-untranslated region (UTR), a 1,854-bp open reading frame (ORF) encoding a protein comprising 617 amino acids with a calculated molecular mass of 68,168 Da, and a 248-bp 3'-UTR followed by a poly(A) tail. The cDNA of the E-subunit consisted of a 72-bp 5'-UTR, a 681-bp ORF encoding a protein of 226 amino acids with a calculated molecular mass of 26,020 Da, and a 799-bp 3'-UTR followed by a poly(A) tail. Western blot and immunofluorescence analyses using specific anti-peptide antisera against the V-ATPase A-and E-subunits revealed that these subunits were present in the ELS, urinary bladder, skin, testes, and kidneys. In the ELS, positive cells were scattered in the follicular epithelium which, as revealed by electron microscopy, corresponds to the location of mitochondria-rich cells. These findings suggest that V-ATPase, including the A-and Esubunits, exists in mitochondria-rich cells of the ELS, which might be involved in dissolution of the calcium carbonate crystals in the lumen of the ELS.

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“…5-10 d before use the toads were transferred to trays with 1-2 cm 0.1 M NaNOs or, in few experiments, with distilled H20. The treatment with C1 -free external solution increases the number of MR-cells (Katz and Gabbay, 1988) and the apical area per cell, thus facilitating the study of the apical membrane. Untreated animals were often found to have so few MRcells and/or so small an apical membrane area that patch-clamp studies were unfeasible.…”

Section: Preparation Of Isolated Mitochondria-rich Cellsmentioning

confidence: 99%

Heterogeneity of chloride channels in the apical membrane of isolated mitochondria-rich cells from toad skin.

Sørensen

Larsen

1996

The Journal of General Physiology

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A B S T RA C T The isolated epithelium of toad skin was disintegrated into single cells by treatment with collagenase and trypsine. Chloride channels of cell-attached and excised inside-out apical membrane-patches of mitochondria-rich cells were studied by the patch-clamp technique. The major population of C1-channels constituted small 7-pS linear channels in symmetrical solutions (125 mM C1-). In cell-attached and inside-out patches the single channel//V-relationship could be described by electrodiffusion of C1-with a Goldmann-Hodgkin-Katz permeability of, Pct = 1.2 • 10 -14 -2.6 3< 10 -14 cm ~ 9 s -1. The channel exhibited voltage-independent activity and could be activated by cAMP. This channel is a likely candidate for mediating the well known cAMP-induced transepithelial C1-conductance of the amphibian skin epithelium. Another population of CI-channels exhibited large, highly variable conductances (upper limit conductances, 150-550 pS) and could be activated by membrane depolarization. A group of intermediate-sized C1--channels included: (a) channels (mean conductance, 30 pS) with linear or slightly outwardly rectifying//V-relationships and activity occurring in distinct "bursts," (b) channels (conductance-range, 10-27 pS) with marked depolarization-induced activity, and (c) channels with unresolvable kinetics. The variance of current fluctuations of such "noisy" patches exhibited a minimum close to the equilibrium-potential for C1-. With channels occurring in only 38% of sealed patches and an even lower frequency of voltage-activated channels, the chloride conductance of the apical membrane of mitochondria-rich cells did not match quantitatively that previously estimated from macroscopic Ussing-chamber experiments. From a qualitative point of view, however, we have succeeded in demonstrating the existence of Cl-channels in the apical membrane with features comparable to macroscopic predictions, i.e., activation of channel gating by cAMP and, in a few patches, also by membrane depolarization.

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Mitochondria-rich cells and carbonic anhydrase content of toad skin epithelium

Cited by 28 publications

References 22 publications

Developmental changes in the heterocellular epidermis of Pelobates syriacus integument

Developmental changes in the heterocellular epidermis of Pelobates syriacus integument

Cloning and Expression of Vacuolar Proton-Pumping ATPase Subunits in the Follicular Epithelium of the Bullfrog Endolymphatic Sac

Heterogeneity of chloride channels in the apical membrane of isolated mitochondria-rich cells from toad skin.

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