Abstract:The mitochondrial electron transport chain was understood as four respiratory complexes (RCs) independently moving in the mitochondrial inner membrane and electrons transferred between them by CoQ and cytochrome c1,2. The disruption of the mitochondrial membranes with mild detergents followed by blue native electrophoresis (BNGE), allowed visualization of RCs as independent entities, but also associated forming a variety of supercomplexes (SCs)1,3. This led to the proposal that RCs are not moving free entities… Show more
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