Mitochondria metabolite transport

Klingenberg, Martin

doi:10.1016/0014-5793(70)80044-8

Cited by 140 publications

(34 citation statements)

References 61 publications

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“…20 The citratelmalate exchange is thus dependent on the pH differential across the membrane, in contrast to the pH-independent malate/Pi exchange. It is interesting to note that the adenine nucleotide exchange across the mitochondrial membrane in the form of an ATP4-/ADP3-exchange is electrogenic a t least to about 50 Ol0 and therefore controlled by a membrane potential [22,3]. Thus all three cases, electroneutral, A pH-dependent and AE-dependent exchange reactions, are realized for mitochondrial metabolite carriers.…”

Section: Electroneutrality Of Anion Exchange Reactionsmentioning

confidence: 99%

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

McGivan

Klingenberg²

1971

European Journal of Biochemistry

158

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The mechanism according to which anions such as di-and tricarboxylates are accumulated in mitochondria are investigated with respect to H+ movement and the role of the Pi carrier.1. I n the absence of respiration, malate is taken up mainly by net accumulation with H+/malate = 1.6. When corrected for exchange with Pi, H+/malate = 2 is found. Under aerobic conditions malate is taken up mainly in exchange for Pi. The ratio H+/malate is largely independent of the pH despite strong pH dependence of the malate uptake.2. The citratelmalate exchange is neutralized by the movement of 1 H+ in the direction of both citrate uptake or efflux.3. The net uptake of malate and citrate is abolished by SH reagents demonstrating a key role of the Pi carrier in the net accumulation of di-and tricarboxylates.4. The uptake of malate and citrate is increased by respiration. This increase is also dependent on the function of the Pi carrier.5. For the movement of Pi a ratio H+/Pi = 1.5 a t pH 6.9 is obtained, in fair agreement with a theoretical H+/Pi a t pH = p K of the dissociation of Pi-to Pi2-.6. Anion distribution not coupled to the Pi carrier, such as the distribution of glutamate, is not linked to H+ movement and to energy-linked accumulation. 7. The role of the H+ gradient and of energy coupling via the Pi carrier in maintaining different redox potentials across the inner mitochondrial membrane is discussed.It is generally accepted that the penetration of substrate anions through the mitochondrial membrane is mediated by specific transport systems. Evidence for the existence of these carriers has been reviewed recently 11-31. The various anion exchanges catalyzed have been clarified by the use of specific inhibitors 14-81, but problems of functional relationship between these anion carriers have not yet been elucidated.The uptake of anions by mitochondria is dependent on pH and it has been shown that the distribution of anions in inhibited mitochondria is quantitatively related to the pH gradient across the membrane [9]. There i s some preliminary evidence that the uptake of Pi is directly coupled to H+ movement while that of malate is not [lo, 111 (cf. also 1121). The uptake of malate and citrate by respiring mitochondria is higher than that by respiration-inhibited mitochondria, and this effect does not depend on cation movement [13].I n this paper, the relationship between substrate anion and Hf movement in tho absence of cation movements is investigated. It is shown here that the pH-dependent uptake of dicarboxylates, oxoglutarate Unusual Abbreviations. Ethylene glycol bis @-aminoethyl)-N,N-tetraacetic acid, EGTA; p-trifluoromethoxycarbonylcyanidephenylhydrazone, FCCP.--and citrate is mediated through the Pi carrier, which has been discussed earlier, as a catalyst for the net uptake of these substrates [13a]. The increased uptake of these anions in the respiring mitochondria is mediated by an effect of energy on Pi transport.The relation of these findings to the redox equilibrium of NAD in the cytoplasm and mitochondria is di...

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Section: Electroneutrality Of Anion Exchange Reactionsmentioning

confidence: 99%

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

McGivan

Klingenberg²

1971

European Journal of Biochemistry

158

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“…Several carrier-mediated anion transporting systems are known to be present in rat liver mitochondria (1,2,4). These are generally considered to be exchange-diffusion carriers or antiporters (5) and include a phosphate-hydroxyl transporter, a dicarboxylate-phosphate transporter, and a tricarboxylatedicarboxylate transporter as outlined in Figure 1.…”

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confidence: 99%

Anion Transporters in Plant Mitochondria

Phillips¹,

Williams²

1973

Plant Physiol.

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“…The dicarboxylic translocator located on the surface of the inner mitochondrial membrane (20) transports malate into the matrix where it is converted to oxaloacetate. The inner membrane, while a selective barrier, is nevertheless permeable to monocarboxylic acids including pyruvate (20) which in the matrix is oxidatively decarboxylated by the pyruvate dehydrogenase complex to acetylCoA. Thus, enzyme location, translocator and diffusion mechanisms provide an efficient system for the synthesis of citrate from malate.…”

Section: Resultsmentioning

confidence: 99%

Photosynthesis in the Higher Plant Vicia faba

Kent¹

1979

Plant Physiol.

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Mitochondria metabolite transport

Cited by 140 publications

References 61 publications

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

Anion Transporters in Plant Mitochondria

Photosynthesis in the Higher Plant Vicia faba

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Mitochondria metabolite transport

Cited by 140 publications

References 61 publications

Correlation Between H+ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

Correlation Between H+ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

Anion Transporters in Plant Mitochondria

Photosynthesis in the Higher Plant Vicia faba

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Product

Resources

About

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier

Correlation Between H⁺ and Anion Movement in Mitochondria and the Key Role of the Phosphate Carrier