Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe

Lee, Min Hee; Park, Nayoung; Yi, Chunsik; Han, Ji Hye; Hong, Ji Hye; Kim, Kwang Pyo; Kang, Dong Hoon; Sessler, Jonathan L.; Kang, Chulhun; Kim, Jong Seung

doi:10.1021/ja506301n

Cited by 397 publications

(258 citation statements)

References 46 publications

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“…The average mitochondria pH of MCF-7 cells observed upon nutrient deprivation was determined to be 6.47 ± 0.26 based on the calibration curve shown in Figure 6 which was lower than that in the MCF-7 cells without nutrient deprivation. The observation of mitochondrial acidification under these conditions is fully consistent with previous reports in the literature, 41 in other words, those cells subjected to nutrient deprivation possess mitochondria characterized by lower pH than those untreated. Therefore, MSNs-EB-AF@SiO 2 -TPP could detect the pH changes in mitochondria and monitor the mitophagy in living MCF-7 cells.…”

Section: Intracellular Imaging Of Ph Fluctuations In Different Organesupporting

confidence: 92%

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Ratiometric Fluorescence Nanoprobes for Subcellular pH Imaging with a Single-Wavelength Excitation in Living Cells

et al. 2016

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Abnormal pH values in the organelles are closely associated with inappropriate cellular functions and many diseases. Monitoring subcellular pH values and their variations is significant in biological processes occurring in living cells and tissues. Herein, we develop a series of ratiometric fluorescence nanoprobes for quantification and imaging of pH values with a single-wavelength excitation in cytoplasm, lysosomes, and mitochondria. The nanoprobes consist of mesoporous silica nanoparticles assembled with aminofluorescein as the recognition unit for pH measurement and ethidium bromide as reference fluorophore. Further conjugation of subcellular targeting moiety enables the nanoprobes to specifically target lysosome and mitochondria. Confocal fluorescence imaging demonstrated that the nanoprobes could effectively monitor the pH fluctuations from 5.0 to 8.3 in living cells by ratio imaging with 488 nm excitation. Subcellular pH determination and imaging in lysosome and mitochondria could also be achieved in different conditions. The current method can offer a general strategy to determine subcellular analytes and investigate the interactions in biological samples.

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Section: Intracellular Imaging Of Ph Fluctuations In Different Organesupporting

confidence: 92%

“…Nutrient deprivation impairs mitochondria function through metabolic inhibition. [41][42][43] It leads to mitochondrial acidification, which in turn is correlated with an increase in mitophagy levels. MCF-7 cells were thus co-incubated with MSNs-EB-AF@SiO 2 -TPP in a serum-free medium.…”

Section: Intracellular Imaging Of Ph Fluctuations In Different Organementioning

confidence: 99%

Ratiometric Fluorescence Nanoprobes for Subcellular pH Imaging with a Single-Wavelength Excitation in Living Cells

et al. 2016

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“…[12] As shown in Figure S1, ATP-Red 1 displayed the strongest fluorescence response to ATP. This may be because the larger steric hindrance of phenylboronic acid at ortho position is favorable for the fluorescent ring-opening structure in the presence of ATP.…”

mentioning

confidence: 88%

A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells

et al. 2015

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Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite-binding switchable fluorescent probe, ATP-Red 1, which selectively and rapidly responds to intracellular concentrations of ATP. Live-cell imaging indicated that ATP-Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP-Red 1, we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell apoptosis. These results indicate that ATP-Red 1 is a useful tool for investigating ATP-relevant biological processes.

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“…The evidence for this has been provided by electron microscopy [130–134], and using confocal microscopy over a timescale ranging from seconds to minutes to follow the movement of fluorescent marker such as the photo-activatable mitochondrial green fluorescent protein [135, 136], mitochondrial red and green fluorescent proteins [137–140], Renilla luciferase complementation [141] to pH-sensitive fluorescent mitochondrial probes [142, 143] between mitochondria.…”

Section: Mitochondrial Morphology and The Adult Heartmentioning

confidence: 99%

Mitochondrial-Shaping Proteins in Cardiac Health and Disease – the Long and the Short of It!

Ong

Kalkhoran

Hernández‐Reséndiz

et al. 2017

Cardiovasc Drugs Ther

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Mitochondrial health is critically dependent on the ability of mitochondria to undergo changes in mitochondrial morphology, a process which is regulated by mitochondrial shaping proteins. Mitochondria undergo fission to generate fragmented discrete organelles, a process which is mediated by the mitochondrial fission proteins (Drp1, hFIS1, Mff and MiD49/51), and is required for cell division, and to remove damaged mitochondria by mitophagy. Mitochondria undergo fusion to form elongated interconnected networks, a process which is orchestrated by the mitochondrial fusion proteins (Mfn1, Mfn2 and OPA1), and which enables the replenishment of damaged mitochondrial DNA. In the adult heart, mitochondria are relatively static, are constrained in their movement, and are characteristically arranged into 3 distinct subpopulations based on their locality and function (subsarcolemmal, myofibrillar, and perinuclear). Although the mitochondria are arranged differently, emerging data supports a role for the mitochondrial shaping proteins in cardiac health and disease. Interestingly, in the adult heart, it appears that the pleiotropic effects of the mitochondrial fusion proteins, Mfn2 (endoplasmic reticulum-tethering, mitophagy) and OPA1 (cristae remodeling, regulation of apoptosis, and energy production) may play more important roles than their pro-fusion effects. In this review article, we provide an overview of the mitochondrial fusion and fission proteins in the adult heart, and highlight their roles as novel therapeutic targets for treating cardiac disease.

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Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe

Cited by 397 publications

References 46 publications

Ratiometric Fluorescence Nanoprobes for Subcellular pH Imaging with a Single-Wavelength Excitation in Living Cells

Ratiometric Fluorescence Nanoprobes for Subcellular pH Imaging with a Single-Wavelength Excitation in Living Cells

A Multisite‐Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells

Mitochondrial-Shaping Proteins in Cardiac Health and Disease – the Long and the Short of It!

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