Mitochondria–actin cytoskeleton crosstalk in cell migration

Yadav, Tarun; Gau, David; Roy, Partha

doi:10.1002/jcp.30729

Cited by 22 publications

(9 citation statements)

References 198 publications

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“…Interestingly, the effects of LARP4A and LARP4B, particularly for epithelial-derived PC3 cells, appear to be independent of any changes in genes associated with epithelial-mesenchymal transition (EMT) which is a widely accepted mechanism of carcinoma cell metastasis. Moreover, the emerging role of mitochondrial activity in regulating cancer cell migration and metastasis [71][72][73] provides a potential novel regulatory link between LARP4A/B mitochondrial localisation and their biological effects.…”

Section: Discussionmentioning

confidence: 99%

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

Coleman

Tattersall

Yianni

et al. 2023

Preprint

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RNA-binding proteins (RBPs) are emerging as important regulators of pathogenesis, including cancer. Here we reveal that the recently characterised RBPs LARP4A and LARP4B are differentially overexpressed in primary osteosarcoma and osteosarcoma lung metastases, as well as in prostate cancer. Depletion of LARP4A and LARP4B inhibited primary tumour growth and metastatic spread in xenograft studies, as well as inhibiting cell proliferation, motility and migration. Transcriptomic profiling combined with high content multiparametric cell cycle analysis unveiled a central role for LARP4B, but not LARP4A, in regulating cell cycle progression in osteosarcoma and prostate cancer cell lines, potentially through modulating the post-transcriptional regulation of RNA targets that include key cell cycle proteins such as Cyclins B1 and E2, Aurora B and E2F1. Our work assigns new functions to LARP4A and LARP4B as pro-tumorigenic proteins in bone and prostate cancer, highlights their similarities while indicating distinct functional aspects, and adds significantly to the rapidly increasing roles of RBPs in different cancer models. Uncovering clear biological roles for these paralogous proteins provides new avenues for identifying novel tissue-specific targets and potential druggable intervention.

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Section: Discussionmentioning

confidence: 99%

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

Coleman

Tattersall

Yianni

et al. 2023

Preprint

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“…( 28,29 ) Physical interactions between the actin cytosketon and mitochondria are known to regulate both cytoskeletal and mitochondrial dynamics. ( 30,31 ) The physiologic relevance of secretome‐induced changes in gene expression are not known, but differences in cell metabolism, rather than proliferation or survival, may explain our results.…”

Section: Discussionmentioning

confidence: 95%

A Conceptual Approach for Examining Effects of the Adolescent Bone Marrow Milieu on MSC Phenotype

et al. 2023

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Children with bone fragility often exhibit elevated bone marrow lipid levels, which may affect mesenchymal stem cell (MSC) differentiation potential and ultimately bone strength via cell-autonomous and/or non-cell-autonomous factors. Here, we use standard co-culture techniques to study biological effects of bone marrow cell-derived secretome on MSC. Bone marrow was collected during routine orthopedic surgery, and the entire marrow cell preparation, with or without red blood cell (RBC) reduction, was plated at three different densities. Conditioned medium (secretome) was collected after 1, 3, and 7 days. ST2 cells, a murine MSC line, were then cultured in the secretomes. Exposure to the secretomes was associated with reductions of up to 62% in MSC MTT outcomes that depended on the duration of secretome development, as well as marrow cell plating density. Reduced MTT values were not associated with diminished cell number and viability assessed using Trypan Blue exclusion. Expression of pyruvate dehydrogenase kinase 4 was modestly elevated, and β-actin levels were transiently reduced in ST2 cells exposed to secretome formulations that had elicited maximal reductions in MTT outcomes. The findings from this study can inform the design of future experimental studies to examine contributions of cellautonomous and non-cell-autonomous factors in the bone marrow to MSC differentiation potential, bone formation, and skeletal growth.

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“…Interestingly, the effects of LARP4A and LARP4B, particularly for epithelial-derived PC3 cells, appear to be independent of any changes in genes associated with epithelial-mesenchymal transition (EMT) which is a widely accepted mechanism of carcinoma cell metastasis. Moreover, the emerging role of mitochondrial activity in regulating cancer cell migration and metastasis 74 , 75 , 76 provides a potential novel regulatory link between LARP4A/B mitochondrial localization and their biological effects which should be explored in further research.…”

Section: Discussionmentioning

confidence: 99%

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

Coleman,

Tattersall,

Yianni

et al. 2024

iScience

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Mitochondria–actin cytoskeleton crosstalk in cell migration

Cited by 22 publications

References 198 publications

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

A Conceptual Approach for Examining Effects of the Adolescent Bone Marrow Milieu on MSC Phenotype

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

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