Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens

Akiyama, Hiroko; Hoshino, Yuko Takada; Itakura, Manabu; Shimomura, Yumi; Yong, Wang; Yamamoto, Akinori; Tago, Kanako; Nakajima, Yasuhiro; Minamisawa, Kiwamu; Hayatsu, Masahito

doi:10.1038/srep32869

Cited by 60 publications

(42 citation statements)

References 31 publications

(59 reference statements)

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“…There are many studies showing N 2 O emissions from symbiotic N 2 fixation plants, such as leguminous crops and acacia trees as being larger than nonsymbiotic N 2 fixation plants (Arai et al 2008;Mori et al 2010;Uchida and Akiyama 2013;Zhang et al 2014). The N 2 O emission from soybean ecosystems could be mitigated by inoculation of high N 2 O reductase N-fixing rhizobium (Akiyama et al 2016). As a symbiotic N 2 fixing plant, how azolla affects N 2 O emission from rice paddy ecosystems as dual crops (rice and azolla covering on flooding water) is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere

Kimani

Cheng

Kanno

et al. 2017

Soil Science and Plant Nutrition

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Azolla (Azolla filiculoides) is a common aquatic fern that has been used successfully as a dual crop with lowland rice. It grows rapidly and has the ability to fix N 2 for rice paddy. However, its ecological significance especially on greenhouse gases emissions remains unclear. To investigate the effect of azolla cover on methane (CH 4 ) and nitrous oxide (N 2 O) emissions from rice paddy, a pot experiment with two treatments, control (rice plant only) and azolla cover (rice plus azolla covering on the flooding water), was carried out in Tsuruoka, Yamagata, Japan, in 2016. The results showed that the rice growth parameters, like shoot height, maximum and productive tiller numbers, and plant biomass were not significantly different between the two treatments. Dual cropping of azolla with rice significantly suppressed CH 4 emissions, likely due to an increase in dissolved oxygen concentration and redox potential at the soil-water interface between flooding water and soil surface. There were significant (P < 0.05) positive correlations between CH 4 flux and night respiration (CO 2 emissions) between the two treatments. The cumulated CH 4 emissions during the growth period until 106 days after transplanting (DAT) was significantly lower at 36.2 g C m −2 from azolla cover treatment than that from control treatment pot at 55.4 g C m −2 . A prolonged nonsignificant N 2 O emission under the azolla cover treatment after the initial highest peak at 15 DAT was recorded due to denitrification of the nitrate in initial soil. No further N 2 O emissions were recorded thereafter from both treatments. Azolla cover did not affect N 2 O emissions from both treatments. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere

Kimani

Cheng

Kanno

et al. 2017

Soil Science and Plant Nutrition

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“…Cupriavidus , Bradyrhizobium , and Azospirillum have been identified in soil as active denitrifying organisms (Akiyama et al. ; Di Palma et al. ; Verbaendert, Hoefman, Boeckx, Boon, & De Vos, ).…”

Section: Resultsmentioning

confidence: 99%

Understanding urban stormwater denitrification in bioretention internal water storage zones

Igielski

Kjellerup

Davis

2019

Water Environment Research

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Conventional free-draining bioretention systems promote nitrate production and continual 23 leaching to receiving waters. In this study, laboratory tests demonstrated the efficacy of an 24 internal water storage zone (IWSZ) to target nitrate removal via denitrification. Experimental 25 results confirmed that the carbon substrate characteristics (Willow Oak woodchip media) and the 26 hydraulic retention time of nitrified stormwater affected nitrate removal performance. A 2.6-day 27 batch treatment time reduced 3.0 mg-N/L to <0.01 mg/L, corresponding to a first-order 28 denitrification rate constant of 0.0011 min-1. Under various flow conditions, the associated 29 hydraulic retention time may be used as a predictive measurement of nitrate removal 30 performance. Scanning Electron Microscopy and 16S rRNA analysis of the woodchips showed that biofilms were present that could be responsible for anaerobic lignocellulose degradation and 32 denitrification. This knowledge, along with evaluation of the biofilm community composition, 33 reinforced the notion of a heterogeneous structure due to nutrient availability and hydrodynamic conditions.

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“…In both season, rice farming is always done in waterlogged status. This provided an interesting insight particularly for Philippines agriculture since USDA110 T was proven to be a highly effective and efficient inoculant for soybean (21, 46) including its complete denitrification ability (2, 14, 41, 43) that is beneficial for climate change mitigation. The locations where these strains were isolated had no history of USDA110 inoculation so these strains are considered indigenous.…”

Section: Discussionmentioning

confidence: 99%

“…Philippines is a tropical country characterized by high temperature, humidity and abundant rainfall. It has two pronounced seasons based mainly from rainfall: (1) dry season – from December to May and (2) rainy season – from June to November. Aside from Baguio City, the average temperature in the country is almost similar at 25.5 – 27.5°C.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

Mason

Tabing

Yamamoto

et al. 2018

Preprint

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18The diversity of indigenous bradyrhizobia from soils collected at 11 locations in the 19 Philippines was investigated using PSB-SY2 local soybean cultivar as the host plant. 20 Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) 21 treatment for 16S rRNA, 16S-23S rRNA internal transcribed spacer (ITS) region and rpoB 22 housekeeping gene was performed primarily to detect the genetic variation among the 424 23 isolates collected. Then, sequence analysis of 16S rRNA, ITS region and rpoB gene was 24 performed for the representative isolates. Majority of the isolates were classified under 25 Bradyrhizobium elkanii, B. diazoefficiens, B. japonicum, Bradyrhizobium sp., and few 26 isolates were related to B. yuanmingense. Genetic variations observed through PCR-RFLP 27 and sequence analyses of the ITS region and rpoB gene generally occurred in B. elkanii, 28 suggesting an occurrence of gene transfer. Shannon's diversity index showed varied results 29 with a lowest score of 0.00 and highest at 0.98 indicating a very diverse population of 30 bradyrhizobia across the country. Among all the factors considered in this work, soil 31 management such as period of flooding and some soil properties provided major influence 32 on the distribution and diversity of soybean bradyrhizobia in the country. Thus, it is 33 proposed that the major micro-symbiont of soybean in the Philippines are B. elkanii for 34 3 non-flooded soils, then B. diazoefficiens and B. japonicum for flooded soils. 35 Importance 36Agriculture production in the Philippines has been and is currently heavily dependent on 37 chemical inputs with mainly rice or corn mono-cropping that it rendered the soil acidic and 38 unproductive. Legume research in the country are mainly focused on plant varietal 39 improvements and very few are aimed at understanding the ecological niche of rhizobia 40 present in the soil. Since soybean has mutual relationship with rhizobia, this legume is a 41 good fallow crop or a rotation crop after rice and corn to help build up the nitrogen stock in 42 the soil. The significance of this research is the better understanding of the ecological niche 43 of indigenous soybean bradyrhizobia, particularly in a tropical archipelago like the 44 Philippines. This work was conceptualized with the utmost goal to increase soybean yield 45 by harnessing and evaluating the indigenous rhizobia in the soil to make production more 46 sustainable and human-friendly. 47 48 KEYWORDS 49 B. elkanii, B. diazoefficiens, B. japonicum, flooded soil, non-flooded, soil management 50 51 52 The genus Bradyrhizobium is a major micro-symbiont of soybean (Glycine max [L.] 53 Merrill) that could form nodule with legumes through symbiosis. The symbiotic 54 relationship between rhizobia and legume is a highly specific, complicated and 55 energy-exhaustive process (50) which may lead to improved crop yield if efficient and 56 sustained. It is considered that Bradyrhizobium is the predominant genus of rhizobia in the 57 tropics (9). Tropical regions ...

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Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens

Cited by 60 publications

References 31 publications

Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere

Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere

Understanding urban stormwater denitrification in bioretention internal water storage zones

Genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

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Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens

Cited by 60 publications

References 31 publications

Azolla cover significantly decreased CH4but not N2O emissions from flooding rice paddy to atmosphere

Azolla cover significantly decreased CH4but not N2O emissions from flooding rice paddy to atmosphere

Understanding urban stormwater denitrification in bioretention internal water storage zones

Genetic diversity and distribution of indigenous soybean-nodulating bradyrhizobia in the Philippines

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Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere

Azolla cover significantly decreased CH₄but not N₂O emissions from flooding rice paddy to atmosphere