Mitigation of paracetamol-induced reproductive damage by chrysin in male rats via reducing oxidative stress

Abstract

Paracetamol (PRC) is a nonsteroidal anti-inflammatory drug used widely as a painkiller for various diseases and as the symptomatic flu cure in several countries worldwide. PRC toxicity may occur under conditions of the overdose usage. Chrysin (CR) is a flavonoid that is naturally present in several plants, honey and propolis. The aim of this study was to investigate the effects of CR (at the doses of 25 mg kg and 50 mg kg ) pre-treatment over seven consecutive days against PRC-induced reproductive toxicity in male rats. Our results showed that PRC toxicity decreased the sperm motility, and increased dead sperm rate, abnormal sperm cell rate, apoptosis and MDA levels in testicular tissues. Pre-treatment with CR at the dose of 25 and 50 mg kg for 7 days mitigated side effects of acute PRC toxicity in male reproductive system proportionally in a dose-dependent manner. This possible protection mechanism might be dependent on the antioxidant activity of CR. In conclusion, pre-treatment with CR at the dose of 25 and 50 mg kg for 7 days can be the beneficial against PRC-induced reproductive toxicity proportionally in a dose-dependent manner.

Karahindiba Ekstresi Yangılı ve Ağrılı Durumlarda Parasetamolün Alternatifi Olabilir mi? Biyokimyasal ve Reprodüktif Parametreler Yönünden Bir Değerlendirme

“…Whereas in overdose, PRC can be detrimental to various tissues [20,[33][34] . High dose PRC transform into NAPQI (N-Acetyl-p-benzoquinone imine) -toxic metabolite by cytochrome p450 enzymes in the biological systems.…”

“…In PRC induced hepatotoxicity studies when toxication group in comparison with control group, it was observed significant increase in MDA level (P<0.05) in rats [39,40] . Aksu et al [20] determined that the decreasing of GSH-px and SOD activity of testicular tissue in the PRC group when compared to the control group in rats. In present study, it is thought that the increasing in MDA level, the decreasing in GSH level, CAT, SOD and GPx activities arise from the harmful effect of PRC via NAPQI pathway.…”

“…Aksu et al [20] showed that PRC toxicity decreased the sperm motility. Similarly, we observed statistically significant reduction in terms of motility in the PRC group in comparison with TOE250 group in the present study.…”

“…To estimate the sperm motility, randomly selected three different fields from each sample were interpreted. The average of three field estimations was calculated as the final motility score of the sample [19,20] . To determine the sperm velocity, the distance of five head positions were measured and were divided into time spent for moving such distance.…”

Erkek Ratlarda Gentamisinin İndüklediği Üreme Hasarına Karşı Karahindiba (Taraxatum officinale) Ekstresinin Koruyucu Etkisi

“…To estimate the sperm motility, randomly selected three different fields from each sample were evaluated. The average of three field estimations was calculated as the final motility score of the sample [15,16] .…”