Mitigation of Cd toxicity by Mn in young plants of cacao, evaluated by the proteomic profiles of leaves and roots

Oliveira, Bruna Rafaela Machado; Almeida, Alex-Alan Furtado de; Pirovani, Carlos Priminho; Barroso, Joedson Pinto; Neto, Carlos Henrique de Carvalho; Ahnert, Dário; Baligar, V. C.; Mangabeira, Pedro Antônio Oliveira

doi:10.1007/s10646-020-02178-4

Cited by 14 publications

(6 citation statements)

References 86 publications

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“…Similar studies carried out on plants, such as Phytolacca americana [ 7 ] and Spinacia oleracea L. [ 28 ], demonstrated that Cd inhibits the activity of RuBisCO. However, in young Theobroma cacao plants, Cd increased the abundance of RuBisCO by sevenfold compared to controls [ 11 ]. Therefore, these results show that Cd stress has significant adverse effects on the efficiency of carbon fixation, which could be related to chlorophyll damage [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…In general, changes in the plant proteome begin with the molecular perception of stress, followed by the activation of signal transduction and the expression of a great variety of genes induced by stress [ 8 , 9 , 10 ]. At the protein level, the overproduction of heat shock proteins (HSPs) occurs during abiotic stress, as they act as chaperones, protecting proteins that have lost their native conformation or have been misfolded during a stress event, and helping to eliminate and degrade the non-corrected proteins [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Changes in Paspalum fasciculatum Leaves Exposed to Cd Stress

Salas-Moreno

Castillejo

Rodríguez-Cavallo

et al. 2022

Plants

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(1) Background: Cadmium is a toxic heavy metal that is widely distributed in water, soil, and air. It is present in agrochemicals, wastewater, battery waste, and volcanic eruptions. Thus, it can be absorbed by plants and enter the trophic chain. P. fasciculatum is a plant with phytoremediation capacity that can tolerate Cd stress, but changes in its proteome related to this tolerance have not yet been identified. (2) Methods: We conducted a quantitative analysis of the proteins present in P. fasciculatum leaves cultivated under greenhouse conditions in mining soils doped with 0 mg kg−1 (control), 30 mg kg−1, or 50 mg kg−1. This was carried out using the label-free shotgun proteomics technique. In this way, we determined the changes in the proteomes of the leaves of these plants, which allowed us to propose some tolerance mechanisms involved in the response to Cd stress. (3) Results: In total, 329 variable proteins were identified between treatments, which were classified into those associated with carbohydrate and energy metabolism; photosynthesis; structure, transport, and metabolism of proteins; antioxidant stress and defense; RNA and DNA processing; and signal transduction. (4) Conclusions: Based on changes in the differences in the leaf protein profiles between treatments, we hypothesize that some proteins associated with signal transduction (Ras-related protein RABA1e), HSPs (heat shock cognate 70 kDa protein 2), growth (actin-7), and cellular development (actin-1) are part of the tolerance response to Cd stress.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomic Changes in Paspalum fasciculatum Leaves Exposed to Cd Stress

Salas-Moreno

Castillejo

Rodríguez-Cavallo

et al. 2022

Plants

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“…Few significant proteins were produced in the presence of Cd while repressed in the presence of Cd + Mn, and vice versa. These findings suggested that Mn mitigated the adverse effects of Cd on cacao plants ( Oliveira et al, 2020 ).…”

Section: Seven-(omics)-based Approaches To Improve Toxic Metals/metalloids Tolerance In Plantsmentioning

confidence: 92%

“…Nevertheless, Mn interaction with Cd and attenuation of the toxic effects on plants are also found to play a major role in photosynthesis. Oliveira et al (2020) studied the mechanisms of Mn-response in Cd toxicity mitigation in young plants of cacao by analyzing the alterations in DAPs and exclusive proteins (EP). Few significant proteins were produced in the presence of Cd while repressed in the presence of Cd + Mn, and vice versa.…”

Section: Seven-(omics)-based Approaches To Improve Toxic Metals/metalloids Tolerance In Plantsmentioning

confidence: 99%

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

et al. 2022

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Food safety has emerged as a high-urgency matter for sustainable agricultural production. Toxic metal contamination of soil and water significantly affects agricultural productivity, which is further aggravated by extreme anthropogenic activities and modern agricultural practices, leaving food safety and human health at risk. In addition to reducing crop production, increased metals/metalloids toxicity also disturbs plants’ demand and supply equilibrium. Counterbalancing toxic metals/metalloids toxicity demands a better understanding of the complex mechanisms at physiological, biochemical, molecular, cellular, and plant level that may result in increased crop productivity. Consequently, plants have established different internal defense mechanisms to cope with the adverse effects of toxic metals/metalloids. Nevertheless, these internal defense mechanisms are not adequate to overwhelm the metals/metalloids toxicity. Plants produce several secondary messengers to trigger cell signaling, activating the numerous transcriptional responses correlated with plant defense. Therefore, the recent advances in omics approaches such as genomics, transcriptomics, proteomics, metabolomics, ionomics, miRNAomics, and phenomics have enabled the characterization of molecular regulators associated with toxic metal tolerance, which can be deployed for developing toxic metal tolerant plants. This review highlights various response strategies adopted by plants to tolerate toxic metals/metalloids toxicity, including physiological, biochemical, and molecular responses. A seven-(omics)-based design is summarized with scientific clues to reveal the stress-responsive genes, proteins, metabolites, miRNAs, trace elements, stress-inducible phenotypes, and metabolic pathways that could potentially help plants to cope up with metals/metalloids toxicity in the face of fluctuating environmental conditions. Finally, some bottlenecks and future directions have also been highlighted, which could enable sustainable agricultural production.

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“…Under metal stress, plants produce endogenous signaling molecules in which signal by ABA perception activates endogenous signal molecules, which result in regulation of the expression and activity of biosynthetic enzymes participated in secondary metabolism, leading to accumulation of target metabolites. Contingent on the states of biotic and abiotic stresses in roots, the gene in control of the synthesis of isoflavone is generated (Oliveira et al 2020).…”

Section: Varied Expression Pattern Of Nucleotide Metabolismmentioning

confidence: 99%

Histological and proteome analyses of Microbacterium foliorum-mediated decrease in arsenic toxicity in Melastoma malabathricum

et al. 2021

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Arsenic (As) is an increasing threat across the globe, widely known as a non-threshold carcinogen, and it is reaching harmful values in several areas of the world. In this study, the effect of plant growth promoting bacteria (Microbacterium foliorum) on inorganic arsenic (Arsenate) phytoremediation by Melastoma malabathricum plants was investigated through histological analysis and proteome profiling of the M. malabathricum plants. Two-dimensional gel electrophoresis and transmission electron microscopy were used to conduct the proteome and histological analysis. When arsenic-treated cells were compared to untreated cells, substantial changes were found (1) severely altered the morphology of the cells, intensely disturbed; (2) the cell wall was thicker; (3) drastically changed the cytoplasm, the cells were polygonal in shape, different in size (scattered), and relatively dense. Compared to the control group, the ultra-structure of the root cells of the control group revealed intact cytoplasm, vacuole, and cell wall under exposure to As + bacteria that had a minor effect on the cell form. To further understand As + bacteria interaction, proteome profiling of the root cell was analyzed. The As-induced oxidative stress enrichment was confirmed by the up-regulation of tubulin, nucleoside diphosphate kinase, and major allergen during As + bacteria exposure It was observed that the profusion of proteins involved in defence, protein biogenesis, signaling, photosynthesis, nucleoside and energy metabolism was greater in As + bacteria as compared to the rooting out of As only. Overall, it can be obviously seen that the current study demonstrates the effectiveness of phytoremediation by M. foliorum on proteins involved and responsivepathways in dealing with As toxicity in M. malabathricum plant.

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Mitigation of Cd toxicity by Mn in young plants of cacao, evaluated by the proteomic profiles of leaves and roots

Cited by 14 publications

References 86 publications

Proteomic Changes in Paspalum fasciculatum Leaves Exposed to Cd Stress

Proteomic Changes in Paspalum fasciculatum Leaves Exposed to Cd Stress

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

Histological and proteome analyses of Microbacterium foliorum-mediated decrease in arsenic toxicity in Melastoma malabathricum

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