Mitigation Effects of Vitamin C and E Against Copper Toxicity in Broiler Chickens, With Reference to Biochemical, Genotoxicity and Pathological Studies

Abstract

Copper (Cu) is necessary for biological utility, nevertheless when existing in abundance; it can produce plentiful injurious impacts. This enquiry was carried out to explore the efficiency of individual or combined doses of vitamin C (Vit C) and vitamin E (Vit E) in ameliorating some biochemical, genotoxicity and pathological changes in the liver of copper sulphate (CuSO 4 )-intoxicated chickens. One hundred- one day old broiler chicks were haphazardly divided into 5groups of 20 chicks each. The broilers were fed on basal diet only (control, gp.1 ) or supplemented with 300 mg CuSO 4 /kg diet (Cu, gp.2 ), CuSO 4 + 250 mg Vit C /kg diet (Cu+ Vit C, gp.3 ), CuSO 4 +250 mg Vit E /kg diet (Cu+ Vit E, gp.4 ) and both vitamins C + E (Cu+ Vit C+ Vit E, gp.5 ) for six weeks. The results displayed that CuSO 4 - intoxicated birds (gp.2) had significantly ( p<0.05 ) dwindled body weight, gain and feed consumption with increased feed conversion rate from week 2 till the 6 th week compared with control group. Serum aminotransferases (ALT, AST), and alkaline phosphatase (ALP) were significantly ( p<0.05 ) augmented in CuSO4- exposed group (gp.2) with significantly ( p<0.05 ) drop in serum total protein (TP), albumin, globulins, triglycerides (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C), and high density lipoprotein-cholesterol (HDL-C) levels compared to control. Concomitantly, histopathological and DNA changes were perceived in liver of CuSO 4 -intoxicated birds. Co-supplementation of Vit C, and Vit E single-handedly or incorporation to CuSO 4 -intoxicated chickens displayed an enhancement in performance traits and abovementioned changes, especially with those given combination of vitamins. From the extant enquiry, it could be established that supplementation of vitamin C and E were beneficial for alleviation the harmful effects of CuSO 4 on performance and other studied parameters in broiler chickens.

Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical, and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens

“…In our previous study, we reported alterations in the liver tissues in Cu-intoxicated chickens represented by hyperplastic and necrotic biliary epithelium with various degenerative and necrotic changes at the third week. Also, cholestasis, necrotic bile duct epithelia, in addition to lymphocytic portal aggregation and fibroblast proliferation, were encountered at the 6th week [15]. Chronic exposure also leads to hemolytic anemia and affects the central nervous system [14,16].…”

“…Both vitamins can function synergistically to inhibit the adverse outcomes of copper toxicity [12]. In our previous study, it was reported that a combination of vitamin C and E can mitigate the histopathological and DNA changes in the liver of CuSO 4 -intoxicated birds [15]. The present study aimed to evaluate the efficacy of single and combined supplementation of vitamin C and vitamin E in mitigating oxidative stress, erythrogram, leukogram changes, and histopathological alterations in the kidney induced by copper sulfate (CuSO 4 ) toxicity in broiler chickens.…”

Dual Junction GaInP/GaAs Solar Cell with Enhanced Efficiency Using Type‐A InP Quantum Wells

“…Growth processes of the top cell are well explained in the reference article. [32] Even though the two materials have a lattice mismatch of about 3-4%, the MOVPE growth process is directed to a very specific growth condition for the nucleation of the coherently strained InP QWs and does not contain dislocations as experimentally demonstrated. [23] So, this structure is feasible from a fabrication point of view.…”

“…τ T becomes insignificant in the QW region due to a very small thickness of the quantum layers and even smaller depletion regions. [32] Increasing the thermionic emission reduces the τ esc , and more carriers will be transported.…”

An Overview of Copper Toxicity and Public Health Concerns with Mitigation Strategies

“…While excessive copper in diet is linked with several detrimental effects on poultry. Supplementation of Cu in excess amount reduced the feed intake, growth and feed conversion ratio in birds (Hashem et al 2021). Numerous studies have verified the harmful effects of copper on birds (Yigit et al 2012;Hefnawy and El-khaiat 2015;Huang et al 2021).…”