Mitigating the negative impact of soluble and insoluble lignin in biorefineries

Solid feeding and co-culture strategies for an efficient enzymatic hydrolysis and ethanol production from sugarcane bagasse

“…Besides the previously reported effects, according to da , the presence of hemicellulose-derived products, like xylose and xylooligomers, can impair CC and reduce glucose yields due to enzyme inhibition. This effect was also observed by Pinto et al (2021), who got lower conversions in the enzymatic hydrolysis when using hydrothermal hydrolysate of sugarcane bagasse, rich in xylose and xylooligomers, instead of buffer solution.…”

“…Despite the many advantages, SSCF presents several bottlenecks regarding combining the optimal conditions for hydrolysis and fermentation, bringing a range of possibilities to be studied and optimized. Great progresses have already been achieved (Silva et al, 2015;Brondi et al, 2021;Pinto et al, 2021;Zhao et al, 2020;Pratto et al, 2020), but there is a lack of studies performed under more realistic conditions for process integration, such as lower temperatures for the hydrolysis and the use of non-conditioned biomass. Moreover, only few studies report the use of non-recombinant strains for pentose and hexose co-fermentation.…”

Toward a Fundamental Understanding of the Role of Lignin in the Biorefinery Process

“…It was reported that these additives could improve glucose release by cellulases treatment by binding to lignin, thus decreased nonproductive cellulases adsorption to lignin (Borjesson et al, 2007;Zheng et al, 2008). Furthermore, biomass washing combined with soybean protein addition was evaluated recently on glucose release during the enzymatic hydrolysis process, which is cost-competitive in a large-scale industrial process (Pinto et al, 2021).…”

Screening of yeast co‐culture using crude hydrolysate for co‐fermentation of pentose and hexose

“…The strains were cultivated in crude hemicellulosic hydrolysate (HH) supplemented with 55 g L ‐1 glucose and 20 g L ‐1 xylose (without nutrients addition), denominated as the HH medium (HHm). The concentrations of glucose and xylose supplemented were defined based on concentrations that could be achieved through the enzymatic hydrolysis of the whole pretreated biomass, considering 15% (w/v) of solid load, and approximately 60% of cellulose and 90% of hemicellulose conversion 28 . The initial pH was adjusted to 5 with NaOH (2 mol/L) solution.…”