Mitigating challenges and expanding the future of vascular tissue engineering—are we there yet?

Shakeel, Adeeba; Corridon, Peter R.

doi:10.3389/fphys.2022.1079421

Cited by 24 publications

(18 citation statements)

References 114 publications

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“…Since such tissues do not possess antigens located at the cell surface, the risk of an adverse immune response is significantly reduced when transplanted to a different host. Decellularization is, therefore, today an important strategy in contemporary transplantology and regenerative medicine research [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Pantić

Cumic

Valjarević

et al. 2023

Preprint

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Decellularized corneas offer a promising and sustainable source of replacement grafts, mimicking native tissue and reducing the risk of immune rejection post-transplantation. Despite great success in achieving acellular scaffolds, little consensus exists regarding the quality of the decellularized extracellular matrix (ECM). Metrics used to evaluate ECM performance are study-specific, subjective, and semi-quantitative. Thus, this work focused on developing a computational method to examine the effectiveness of corneal decellularization. We combined conventional semi-quantitative histological assessments and automated scaffold evaluations based on textual image analyses to assess decellularization efficiency. Our study highlights that it is possible to develop contemporary artificial intelligence (AI) models based on random forests and support vector machine algorithms, which can identify regions of interest in acellularized corneal stromal tissue with relatively high accuracy. These results provide a platform for developing AI biosensing systems for evaluating fine morphological changes in decellularized scaffolds crucial for evaluating their functionality.

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Section: Introductionmentioning

confidence: 99%

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Pantić

Cumic

Valjarević

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Also, as anticipated, histological assessments allowed the visualization of nuclei and the ECM components (primarily collagen) in native sections. In comparison, such assessments highlight the effective removal of cellular compartments and retention of ECM components and confirm successful decellularization (1,11,(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 85%

A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste

Wang¹,

Shakeel²,

Salih³

et al. 2023

Preprint

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High-throughput platforms that can generate copious quantities of corneal grafts will be invaluable in addressing the existing global demand for keratoplasty. Slaughterhouses generate substantial underutilized biological waste that can be repurposed to reduce current environmentally unfriendly practices. Such efforts to support ecological sustainability can simultaneously drive the development of transplantable ovine corneas and patient-specific bioartificial keratoprostheses. This study presents a dataset highlighting techniques that pioneered the development of a simplified, inexpensive, and high-throughput corneal xenograft platform. To the best of our knowledge, this is the first dataset to be generated in the United Arab Emirates devised on repurposing slaughterhouse waste in a manner that supports circular economic practices. These efforts outline the first step in developing a platform that can produce dozens of native ovine xenografts and acellular corneal scaffolds that effectively preserve ocular transparency, transmittance, and ECM components, and can be used to potentially create individualized and transplantable grafts. Such native grafts can then be genetically modified to reduce rejection and retrovirus transmission in recipients. Analogously, decellularization technologies can support corneal regeneration with key attributes comparable to native xenografts.

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“…This form of therapy provides a means to treat and prevent the root cause of a given condition. Over the years, several methods have been proposed to elicit efficient gene transfer in various settings 75,77,[86][87][88][89][90][91][92][93] . However, progress in the development of direct gene transfer methods in the kidney has been limited compared with other organs.…”

Section: Discussionmentioning

confidence: 99%

Enhancing the expression of a key mitochondrial enzyme at the inception of ischemia-reperfusion injury can boost recovery and halt the progression of acute kidney injury

Corridon

2023

Preprint

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Hydrodynamic fluid delivery has shown promise in influencing renal function in disease models. This technique provided pre-conditioned protection in acute injury models by upregulating the mitochondrial adaptation, while hydrodynamic injections of saline alone have improved microvascular perfusion. Accordingly, hydrodynamic mitochondrial gene delivery was applied to investigate the ability to halt progressive or persistent renal function impairment following episodes of ischemia-reperfusion injuries known to induce acute kidney injury (AKI). The rate of transgene expression was approximately 33% and 30% in rats with prerenal AKI that received treatments 1 (T1hr) and 24 (T24hr) hours after the injury was established, respectively. The resulting mitochondrial adaptation via exogenous IDH2 (isocitrate dehydrogenase 2 (NADP+) and mitochondrial) significantly blunted the effects of injury within 24 hours of administration: decreased serum creatinine (» 60%, p < 0.05 at T1hr; » 50%, p < 0.05 at T24hr) and blood urea nitrogen (»50%, p < 0.05 at T1hr; » 35%, p < 0.05 at T24hr) levels, and increased urine output (» 40%, p < 0.05 at T1hr; » 26%, p < 0.05 at T24hr) and mitochondrial membrane potential, Δψm, (» by a factor of 13, p < 0.001 at T1hr; » by a factor of 11, p < 0.001 at T24hr), despite elevated histology injury score (26%, p < 0.05 at T1hr; 47%, p < 0.05 at T24hr). Therefore, this study identifies an approach that can boost recovery and halt the progression of AKI at its inception.

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Mitigating challenges and expanding the future of vascular tissue engineering—are we there yet?

Cited by 24 publications

References 114 publications

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

Computational approaches for evaluating morphological changes in the corneal stroma associated with decellularization

A scalable corneal xenograft platform: simultaneous opportunities for tissue engineering and circular economic sustainability by repurposing slaughterhouse waste

Enhancing the expression of a key mitochondrial enzyme at the inception of ischemia-reperfusion injury can boost recovery and halt the progression of acute kidney injury

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