Mithramycin A suppresses expression of the human melanoma-associated gene ABCB8

Sachrajda, Iwona; Ratajewski, Marcin

doi:10.1007/s00438-010-0586-8

Cited by 11 publications

(11 citation statements)

References 44 publications

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“…AAEL006717) of human ABCB7 in the dengue vector, Aedes aegypti , was reported to be upregulated in an insecticide resistant strain [9,63]. The function of human ABCB8 is not well understood, but it was shown to mediate resistance against the chemotherapeutic agent doxorubicin in melanoma cells [64,65]. Also, Ichikawa et al found that disruption of the mouse orthologue of human ABCB8 lead to cardiomyopathy and decreased mitochondrial iron export [66].…”

Section: Resultsmentioning

confidence: 99%

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

et al. 2013

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BackgroundThe ABC (ATP-binding cassette) gene superfamily is widespread across all living species. The majority of ABC genes encode ABC transporters, which are membrane-spanning proteins capable of transferring substrates across biological membranes by hydrolyzing ATP. Although ABC transporters have often been associated with resistance to drugs and toxic compounds, within the Arthropoda ABC gene families have only been characterized in detail in several insects and a crustacean. In this study, we report a genome-wide survey and expression analysis of the ABC gene superfamily in the spider mite, Tetranychus urticae, a chelicerate ~ 450 million years diverged from other Arthropod lineages. T. urticae is a major agricultural pest, and is among of the most polyphagous arthropod herbivores known. The species resists a staggering array of toxic plant secondary metabolites, and has developed resistance to all major classes of pesticides in use for its control.ResultsWe identified 103 ABC genes in the T. urticae genome, the highest number discovered in a metazoan species to date. Within the T. urticae ABC gene set, all members of the eight currently described subfamilies (A to H) were detected. A phylogenetic analysis revealed that the high number of ABC genes in T. urticae is due primarily to lineage-specific expansions of ABC genes within the ABCC, ABCG and ABCH subfamilies. In particular, the ABCC subfamily harbors the highest number of T. urticae ABC genes (39). In a comparative genomic analysis, we found clear orthologous relationships between a subset of T. urticae ABC proteins and ABC proteins in both vertebrates and invertebrates known to be involved in fundamental cellular processes. These included members of the ABCB-half transporters, and the ABCD, ABCE and ABCF families. Furthermore, one-to-one orthologues could be distinguished between T. urticae proteins and human ABCC10, ABCG5 and ABCG8, the Drosophila melanogaster sulfonylurea receptor and ecdysone-regulated transporter E23. Finally, expression profiling revealed that ABC genes in the ABCC, ABCG ABCH subfamilies were differentially expressed in multi-pesticide resistant mite strains and/or in mites transferred to challenging (toxic) host plants.ConclusionsIn this study we present the first comprehensive analysis of ABC genes in a polyphagous arthropod herbivore. We demonstrate that the broad plant host range and high levels of pesticide resistance in T. urticae are associated with lineage-specific expansions of ABC genes, many of which respond transcriptionally to xenobiotic exposure. This ABC catalogue will serve as a basis for future biochemical and toxicological studies. Obtaining functional evidence that these ABC subfamilies contribute to xenobiotic tolerance should be the priority of future research.

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Section: Resultsmentioning

confidence: 99%

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

et al. 2013

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“…Mith suppresses the expression of the human melanomaassociated gene ABCB8 and has potential therapeutic applications for treating melanoma patients (Sachrajda and Ratajewski, 2011). Mith also decreased VEGF and XIAP to induce apoptosis and inhibit tumor cell migration in glioma cells (Seznec et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Mithramycin A Inhibits Myeloid Cell Leukemia-1 to Induce Apoptosis in Oral Squamous Cell Carcinomas and Tumor Xenograft through Activation of Bax and Oligomerization

et al. 2012

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“…We screened a chemical library of 1120 compounds using cells that were transfected with the previously described ABCB8 promoter construct [30]. The screening resulted in the identification of approximately 20 compounds that activated or repressed the transcriptional properties of the ABCB8 promoter (data not shown).…”

Section: Ac-93253 Influences the Expression Of The Abc Melanoma Gene mentioning

confidence: 99%

AC-93253 triggers the downregulation of melanoma progression markers and the inhibition of melanoma cell proliferation

Karwaciak

Gorzkiewicz

Ryba

et al. 2015

Chemico-Biological Interactions

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Mithramycin A suppresses expression of the human melanoma-associated gene ABCB8

Cited by 11 publications

References 44 publications

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

A burst of ABC genes in the genome of the polyphagous spider mite Tetranychus urticae

Mithramycin A Inhibits Myeloid Cell Leukemia-1 to Induce Apoptosis in Oral Squamous Cell Carcinomas and Tumor Xenograft through Activation of Bax and Oligomerization

AC-93253 triggers the downregulation of melanoma progression markers and the inhibition of melanoma cell proliferation

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