Mites (Acari: Trombidiformes) parasitizing mosquitoes (Diptera: Culicidae) in an Atlantic Forest area in southern Brazil with a new mite genus country record

Santos, Emili Bortolon dos; Favretto, Mario Arthur; Costa, Samuel Geremias Dos Santos; Navarro-Silva, Mário Antônio

doi:10.1007/s10493-016-0045-2

Cited by 7 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 6 , 10 , 31 , 32 ]). Arrenurus is the genus most reported to parasitize mosquitoes, evidence of its flexibility regarding host specificity [ 33 ]. Despite this, recent findings suggest that these mites prefer Culex species [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Correlation between Larval, Deutonymph and Adult Stages of the Water Mite Arrenurus (Micruracarus) Novus

Alarcón-Elbal

García‐Jiménez

Peláez

et al. 2020

Life

View full text Add to dashboard Cite

The systematics of many groups of organisms has been based on the adult stage. Morphological transformations that occur during development from the embryonic to the adult stage make it difficult (or impossible) to identify a juvenile (larval) stage in some species. Hydrachnidia (Acari, Actinotrichida, which inhabit mainly continental waters) are characterized by three main active stages—larval, deutonymph and adult—with intermediate dormant stages. Deutonymphs and adults may be identified through diagnostic morphological characters. Larvae that have not been tracked directly from a gravid female are difficult to identify to the species level. In this work, we compared the morphology of five water mite larvae and obtained the molecular sequences of that found on a pupa of the common mosquito Culex (Culex) pipiens with the sequences of 51 adults diagnosed as Arrenurus species and identified the undescribed larvae as Arrenurus (Micruracarus) novus. Further corroborating this finding, adult A. novus was found thriving in the same mosquito habitat. We established the identity of adult and deutonymph A. novus by morphology and by correlating COI and cytB sequences of the water mites at the larval, deutonymph and adult (both male and female) life stages in a particular case of ‘reverse taxonomy’. In addition, we constructed the Arrenuridae phylogeny based on mitochondrial DNA, which supports the idea that three Arrenurus subgenera are 'natural': Arrenurus, Megaluracarus and Micruracarus, and the somewhat arbitrary distinction of the species assigned to the subgenus Truncaturus.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Correlation between Larval, Deutonymph and Adult Stages of the Water Mite Arrenurus (Micruracarus) Novus

Alarcón-Elbal

García‐Jiménez

Peláez

et al. 2020

Life

View full text Add to dashboard Cite

show abstract

“…A vertebrate host was considered as identified when sequence similarity was ≥ 98%. As some species of Culicidae that were present in the PEP [48] did not contain genetic data in GenBank or BOLD, by standardization, we considered as identified species only those with similarity equal to 100%. Similarly values below those cut-off values were considered as identification to the genus or subgenus levels.…”

Section: Methodsmentioning

confidence: 99%

Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil

et al. 2019

Self Cite

View full text Add to dashboard Cite

The study of host associations of mosquitoes (Diptera, Culicidae) provides valuable information to assist in our understanding of a variety of related issues, from their life-history to the entomological surveillance of pathogens. In this study, we identified and characterized mosquito blood meals from both urban and forested areas in the city of Paranaguá, state of Paraná, Brazil, by analyzing the amplification of host DNA ingested by mosquitoes under different storage conditions and digestion levels. Host DNA preservation was evaluated in fresh blood meals according to storage duration (30 to 180 days) and temperature (-20°C / -80°C) and, in digested blood, according the degree of digestion classified on the Sella scale. Molecular analysis of blood meals was based on DNA extraction and amplification of a fragment of the mitochondrial COI gene. We determined that, up to180 days of storage, the evaluated temperatures did not influence the preservation of fresh blood meals DNA, whereas the amplification success was increasingly reduced over the course of the digestion process. The species Anopheles cruzii , Aedes fluviatilis , Aedes scapularis , Psorophora ferox , Culex quinquefasciatus , Culex mollis , and Culex intrincatus , together with specimens representing four subgenera and one genus of Culicidae [ Ae . ( Ochlerotatus ), Cx . ( Culex ), Cx . ( Melanoconion ), Cx . ( Microculex ), and Limatus , respectively] had their blood meals identified. Their diverse host use was evidenced by the identification of 19 species of vertebrate host, namely two amphibians, three mammals and 14 birds. Birds were the most commonly identified host in blood meals. These results not only show the diversity of mosquito hosts, but also underscore the challenges involved in monitoring arboviruses of public health importance, given potential combinations of host use for each mosquito species.

show abstract

“…Conversely, parasitism of mosquitoes by mites is usually associated with adverse effects on mosquito fitness (i.e. reduced reproductive ability and survival) ( Dos Santos et al. 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Meta-transcriptomics reveals potential virus transfer between Aedes communis mosquitoes and their parasitic water mites

et al. 2022

View full text Add to dashboard Cite

Arthropods harbor a largely undocumented diversity of RNA viruses. Some arthropods, like mosquitoes, can transmit viruses to vertebrates, but are themselves parasitized by other arthropod species, such as mites. Very little is known about the viruses of these ectoparasites and how they move through the host-parasite relationship. To address this, we determined the virome of both mosquitoes and the mites that feed on them. The mosquito Aedes communis is an abundant and widely distributed species in Sweden, in northern Europe. These dipterans are commonly parasitized by water mite larvae (Trombidiformes: Mideopsidae) that are hypothesized to impose negative selection pressures on the mosquito by reducing fitness. In turn, viruses are dual-host agents in the mosquito-mite interaction. We determined the RNA virus diversity of mite-free and mite-detached mosquitoes, as well as in their parasitic mites, using meta-transcriptomic sequencing. Our results revealed an extensive RNA virus diversity in both mites and mosquitoes, including 37 putative novel RNA viruses that cover a wide taxonomic range. Notably, a high proportion of viruses (20/37) were shared between mites and mosquitoes, while a limited number of viruses were present in a single host. Comparisons of virus composition and abundance suggest potential virus transfer between mosquitoes and mites during their symbiotic interaction. These findings shed light on virome diversity and ecology in the context of arthropod host-parasite-virus relationships.

show abstract

Mites (Acari: Trombidiformes) parasitizing mosquitoes (Diptera: Culicidae) in an Atlantic Forest area in southern Brazil with a new mite genus country record

Cited by 7 publications

References 35 publications

Molecular Correlation between Larval, Deutonymph and Adult Stages of the Water Mite Arrenurus (Micruracarus) Novus

Molecular Correlation between Larval, Deutonymph and Adult Stages of the Water Mite Arrenurus (Micruracarus) Novus

Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil

Meta-transcriptomics reveals potential virus transfer between Aedes communis mosquitoes and their parasitic water mites

Contact Info

Product

Resources

About