Mite Sarcoptes scabiei Varieties Hominis in South Sumatra: Specific Identification and Comparative Study

Abstract

BACKGROUND: Sarcoptes scabiei mites have more than 15 genetically diverse varieties from various hosts. Identification of S. scabiei mite varieties hominis as an intervention in its prevention is still rarely done.
 AIM: This study aimed to observe the genetic relationship of the mite S. scabiei varieties hominis compare to the parasite S. scabiei varieties hominis in other regions.
 METHODS: This research used polymerase chain reaction (PCR) and sequencing methods with 16S gene-specific primers. From 32 S. scabiei samples, 22 samples were identified as varieties hominis that was marked by the appearance of the band at 132 bp.
 RESULTS: S. scabiei mites hominis varieties from South Sumatra (Yessi Scabies A2 and Yessi Scabies B3) have similarities with deoxyribonucleic acid (DNA) strands with S. scabiei hominis varieties from China (KJ781377 and KJ781376). In contrast, Yessi Scabies A1 has similarities with DNA strands with mite S. scabiei varieties hominis from Australia (AY493402). Still, all the DNA strands, this research is different from S. scabiei mites DNA strands hominis from Panama and Pakistan.
 CONCLUSION: The PCR method is advantageous and specific in identifying mites S. scabiei hominis varieties, the cause of scabies in humans.