Mite-induced Inflammation: More than Allergy

Sánchez‐Borges, Mario; Fernández-Caldas, Enrique; Capriles‐Hulett, Arnaldo; Caballero‐Fonseca, Fernan

doi:10.2500/ar.2012.3.0025

Cited by 30 publications

(18 citation statements)

References 47 publications

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“…rDer p 1 was found to significantly change the expression of PTGS1 and PTGS2 genes, and in this regard our results contradict those of Sánchez-Borges et al [55] where HDM extract inhibited COX-1. The authors reported that this effect can be caused by COX-1-inhibitory substances in commercial mite allergenic extracts rather the allergen alone; the lack of any inhibitory effect observed in the present study can be explained by our use of the recombinant allergen (rDer p 1).…”

Section: Discussioncontrasting

confidence: 57%

Exacerbating Factors Induce Different Gene Expression Profiles in Peripheral Blood Mononuclear Cells from Asthmatics, Patients with Chronic Obstructive Pulmonary Disease and Healthy Subjects

Pniewska

Sokołowska²,

Kupryś‐Lipińska³

et al. 2014

Int Arch Allergy Immunol

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Background: Despite several common phenotypic features, chronic obstructive pulmonary disease (COPD) and severe asthma differ with regard to their causative factors and pathophysiology. Both diseases may be exacerbated by environmental factors, however, the molecular profiles of disease episodes have not been comprehensively studied. We identified differences in gene and protein expression profiles expressed by peripheral blood mononuclear cells (PBMC) of COPD patients, patients with atopic asthma and healthy subjects when challenged with exacerbating factors in vitro: lipopolysaccharide (LPS), house dust mite (HDM) and cat allergen. Methods: PBMC isolated from patients with severe atopic asthma and COPD, as well as healthy subjects were stimulated with rDer p 1 DG, rFel d 1 DG and LPS. The changes in the expression of 47 genes belonging to five groups (phospholipase A₂, eicosanoids, transcription factors, cytokines and airway remodeling) were studied using TaqMan low density array cards. Immunoblotting was used to study relative protein expression. Results: rDer p 1 significantly up-regulated the expression of PLA2G4A, PLA2G6, PLA2G15, CYSLTR1, LB4R2, PTGS1, PTGS2, FOXP1, GATA3, HDAC2, IREB2, PPARG, STAT4, TSLP and CHI3L1 genes in asthmatics in comparison to healthy subjects. LPS induced significant expression of ANXA1 and LTA4H in asthmatics when compared to COPD patients and healthy subjects. SOX6,STAT4 and IL1RL1 were induced in COPD after LPS stimulation. Analysis of protein expression revealed a pattern similar to mRNA expression. Conclusions: LPS-induced exacerbation of asthma and COPD is characterized by differential expression of selected genes in PBMC. HDM allergen changed the expression profile of inflammatory genes between patients with asthma of atopic origin and healthy controls.

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Section: Discussioncontrasting

confidence: 57%

Exacerbating Factors Induce Different Gene Expression Profiles in Peripheral Blood Mononuclear Cells from Asthmatics, Patients with Chronic Obstructive Pulmonary Disease and Healthy Subjects

Pniewska

Sokołowska²,

Kupryś‐Lipińska³

et al. 2014

Int Arch Allergy Immunol

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“…1,11,15---17 An association particularly with house dust mite sensitisation has been described. 18 The exact mechanism remains unclear. 15,17 However, this association was not found by other authors.…”

Section: Discussionmentioning

confidence: 99%

Non-steroidal anti-inflammatory drug hypersensitivity in children

Alves

Romeira

Abreu

et al. 2017

Allergologia et Immunopathologia

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Introduction: There are rather few publications about hypersensitivity reactions to nonsteroidal anti-inflammatory drugs (NSAID) in the paediatric age. In this study, we aimed to assess the frequency of confirmed NSAID hypersensitivity in children with a previous reported reaction to NSAID in order to investigate the role of the drug provocation test (DPT) in the diagnostic workup and to explore the factors associated with confirmed NSAID hypersensitivity. Methods: We conducted a retrospective analysis of the clinical files from every patient under 18 years old who attended two Portuguese paediatric allergy outpatient clinics, from January 2009 to August 2014, due to a suspected NSAID hypersensitivity. Results: We included 119 patients, with a median age of nine years (P 25 ---P 75 : 5---14). Ibuprofen was the commonest implicated NSAID in the patients' reports (n = 94---79%). After DPT, NSAID hypersensitivity was confirmed in nine (7.6%) patients, excluded in 93 (78.2%) and was inconclusive in 17 (14.3%). In the majority (n = 95---79.8%), the reaction occurred in the first 24 h after intake. Eighty-four patients (70.6%) reported only cutaneous manifestations and 18 (15.1%) had systemic symptoms. Anaphylaxis represented a relative risk to NSAID hypersensitivity confirmation. No association was found for atopy and the number of previous reactions. Conclusion: In our study, NSAID hypersensitivity was confirmed in a small proportion of the patients with a previous reported reaction. Ibuprofen was the most implicated drug with urticaria/angio-oedema as the commonest manifestation. Anaphylaxis was associated with confirmed drug hypersensitivity. The drug provocation test was essential to establish the diagnosis.

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“…The latter is most frequently induced by these drugs, responsible for around 60% of reactions [8]. Intriguingly, over 60% of NIUA patients are atopic although the reason is still unknown [8,9]. NERD and NECD are considered to be caused by a nonspecific immunological mechanism whereby NSAIDs inhibit COX enzymes, leading to a dysregulation of leukotriene homeostasis [7].…”

Section: Introductionmentioning

confidence: 98%

Copy number variation in ALOX5 and PTGER1 is associated with NSAIDs-induced urticaria and/or angioedema

Plaza-Serón

Ayuso

Pérez‐Sánchez

et al. 2016

Pharmacogenetics and Genomics

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A total of 151 cases and 139 controls were analyzed during the discovery phase and 148 cases and 140 controls were used for replication. CNVs in open reading frames were found for ALOX5, PTGER1, PTGER3, and PTGER4. Statistically significant differences in the CNV frequency between NIUA and controls were found for ALOX5 (Pc=0.017) and PTGER1 (Pc=1.22E-04). This study represents the first analysis showing an association between CNVs in exonic regions of ALOX5 and PTGER1 and NIUA. This suggests a role of CNVs in this pathology that should be explored further.

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Mite-induced Inflammation: More than Allergy

Cited by 30 publications

References 47 publications

Exacerbating Factors Induce Different Gene Expression Profiles in Peripheral Blood Mononuclear Cells from Asthmatics, Patients with Chronic Obstructive Pulmonary Disease and Healthy Subjects

Exacerbating Factors Induce Different Gene Expression Profiles in Peripheral Blood Mononuclear Cells from Asthmatics, Patients with Chronic Obstructive Pulmonary Disease and Healthy Subjects

Non-steroidal anti-inflammatory drug hypersensitivity in children

Copy number variation in ALOX5 and PTGER1 is associated with NSAIDs-induced urticaria and/or angioedema

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