Mite-Induced Allergic Airway Inflammation in Guinea Pigs

Hsiue, Tzuen Ren; Lei, Huan Yao; Hsieh, Ai Ling; Wang, Tzu Yao; Chang, Hsing Cheng; Chen, Chen Ren

doi:10.1159/000237469

Cited by 20 publications

(11 citation statements)

References 19 publications

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“…Among the many aeroallergens, house dust mites are the most common [3], and any approach to the experimental study of house dust mite allergy to improve medical treatment requires a suitable animal model, such as the mouse or guinea pig [4,5].…”

Section: Introductionmentioning

confidence: 99%

Experimental Monkey Model Sensitized with Mite Antigen

Yasue

Nakamura

Yokota

et al. 1998

Int Arch Allergy Immunol

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Background: Monkeys are considered to have an immune system very similar to that of humans, as compared with mice, rats, and guinea pigs. Although primate allergic models to several pollen allergens have been developed, no model of house dust mite allergy has been reported. In this study, we attempted to induce type I allergy to mite allergens in rhesus monkeys. Methods: Six rhesus monkeys were immunized subcutaneously with crude mite extract adsorbed on aluminum hydroxide for 4 months. Then 5 monkeys positive for IgE production to mite extract were further immunized subcutaneously and conjunctivally with recombinant Der f 2 (rDer f 2). The status of sensitization to mite extract and rDer f 2 in monkeys was examined before and after the immunization. Plasma antigen-specific IgE and IgG levels, cutaneous reaction, and histamine release from peripheral blood leukocytes were measured. After conjunctival immunization, immediate conjunctivitis and leukocyte influx into conjunctiva after rDer f 2 challenge were examined. Results: After immunization with crude mite extract, 5 of 6 sensitized monkeys showed IgE response to the mite, and 4 out of 5 rDer f 2-sensitized monkeys exhibited IgE production to rDer f 2. Three monkeys sensitized with rDer f 2 showed immediate conjunctivitis and conjunctival eosinophilia after applying rDer f 2 to their eyes. Sensitized animals also showed IgG response to mite antigens. Conclusion: Four rhesus monkeys were positive for IgE production and allergic reactions to both mite extract and rDer f 2. These monkeys could represent a useful model for studying the development and regulation of house dust mite allergy.

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Section: Introductionmentioning

confidence: 99%

Experimental Monkey Model Sensitized with Mite Antigen

Yasue

Nakamura

Yokota

et al. 1998

Int Arch Allergy Immunol

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“…To date, the majority of work, in animal models, relating to allergy to HDM Ags has been performed in immunologically deficient animals in the form of a humanized SCID mouse model (8,9) or in either of the above-mentioned conventional models (10 -13). In this regard, Hsiue et al (14) showed that multiple aerosol inhalations of crude mite extract were insufficient to cause sensitization in guinea pigs and that, in fact, i.p. injections were required to elicit overt Th2 sensitization.…”

mentioning

confidence: 99%

Intranasal Exposure of Mice to House Dust Mite Elicits Allergic Airway Inflammation via a GM-CSF-Mediated Mechanism

Cates

Fattouh

Wattie

et al. 2004

The Journal of Immunology

246

141

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It is now well established that passive exposure to inhaled OVA leads to a state of immunological tolerance. Therefore, to elicit allergic sensitization, researchers have been compelled to devise alternative strategies, such as the systemic delivery of OVA in the context of powerful adjuvants, which are alien to the way humans are exposed and sensitized to allergens. The objectives of these studies were to investigate immune-inflammatory responses to intranasal delivery of a purified house dust mite (HDM) extract and to evaluate the role of GM-CSF in this process. HDM was delivered to BALB/c mice daily for 10 days. After the last exposure, mice were killed, bronchoalveolar lavage was performed, and samples were obtained. Expression/production of Th2-associated molecules in the lymph nodes, lung, and spleen were evaluated by real-time quantitative PCR and ELISA, respectively. Using this exposure protocol, exposure to HDM alone generated Th2 sensitization based on the expression/production of Th2 effector molecules and airway eosinophilic inflammation. Flow cytometric analysis demonstrated expansion and activation of APCs in the lung and an influx of activated Th2 effector cells. Moreover, this inflammation was accompanied by airways hyper-responsiveness and a robust memory-driven immune response. Finally, administration of anti-GM-CSF-neutralizing Abs markedly reduced immune-inflammatory responses in both lung and spleen. Thus, intranasal delivery of HDM results in Th2 sensitization and airway eosinophilic inflammation that appear to be mediated, at least in part, by endogenous GM-CSF production.

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“…In this study, aerosol challenge of intraperitoneally mite–sensitized animals caused eosinophilia in BAL fluid, which peaked 24 h after the aerosol challenge. Eosinophilia was generally sustained for at least 1 week, and subsided after 2 weeks [13]. …”

Section: Discussionmentioning

confidence: 99%

“…They were actively sensitized with an intraperitoneal injection of 100 µg CME with 4 mg Al(OH) 3 in 1 ml normal saline on days 0 and 7 [13]. …”

Section: Methodsmentioning

confidence: 99%

Time Course of Pharmacological Modulation of Peak Eosinophilic Airway Inflammation after Mite Challenge in Guinea Pigs: A Therapeutic Approach

Hsiue

Lei

Hsieh

et al. 1999

Int Arch Allergy Immunol

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Background: It is well known that eosinophilic airway inflammation develops after allergen challenge in sensitized humans and animals. However, the detailed time course of suppression of early eosinophilic airway inflammation by pharmacological agents given just after challenge has not been discussed. Therefore, we aimed to evaluate the time course relationship of the suppression of peak eosinophilia by anti–cytokines and pharmacological agents given several hours after the aerosol challenge by a therapeutic approach. Methods: We used crude mite extract as an allergen to create a sensitization and inhalation challenge, and performed bronchoalveolar lavages (BAL) after the inhalation challenge to observe the degree of eosinophilic airway inflammation in guinea pigs. Various anti–cytokines (anti–IL–3 and anti–IL–5) and pharmacological agents (dexamethasone, theophylline, and roxithromycin) were given within several hours after the acute aeorosol challenge to evaluate the suppressive effect on peak eosinophilia in BAL fluid, which occurred 24 h after the challenge. Results: Our results show that anti–IL–5 and dexamethasone, given within 4 and 8 h after the inhalation challenge, respectively, inhibit the acute allergen–induced peak eosinophilia in BAL fluid. However, anti–IL–3, theophylline, and roxithromycin had no effect on peak eosinophilic airway inflammation after challenge. Conclusion: These observations suggest that several hours are needed to complete the process of cytokine–induced recruitment of eosinophils from the blood to the airways after acute allergen challenge. This may be the optimal time to administer anti–cytokines and dexamethasone to attenuate the subsequent eosinophilic airway inflammation after acute allergen–induced asthmatic attacks.

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Mite-Induced Allergic Airway Inflammation in Guinea Pigs

Cited by 20 publications

References 19 publications

Experimental Monkey Model Sensitized with Mite Antigen

Experimental Monkey Model Sensitized with Mite Antigen

Intranasal Exposure of Mice to House Dust Mite Elicits Allergic Airway Inflammation via a GM-CSF-Mediated Mechanism

Time Course of Pharmacological Modulation of Peak Eosinophilic Airway Inflammation after Mite Challenge in Guinea Pigs: A Therapeutic Approach

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