Mite allergen (Der p 1) is not only carried on mite feces

Lucca, Sandra D. De; Sporik, Richard; O'Meara, T.J.; Tovey, Euan R.

doi:10.1016/s0091-6749(99)70543-9

Cited by 38 publications

(27 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Personal monitoring of allergen entering the airways would considerably improve exposure assessment. Techniques determining nasal HDM allergen exposure as well as the size of the partides on which allergen enters the airways have recently been presented in preliminary reports (20,21 …”

Section: Methods Study Designmentioning

confidence: 99%

Variability of house dust mite allergen exposure in dwellings.

Hirsch¹,

Kuhlisch²,

Soldan³

et al. 1998

Environ Health Perspect

View full text Add to dashboard Cite

Tlhe variability of repeated house dust mite (HDM) allergen determinations at the same site within 3-24 months was evaluated on previously collected samples. Between two and four repeated measurements of Der p 1, a major allergen of Dermatophagoidespteronyssinus and Der f 1, a major allergen of D. farinae, on 46 carpets and 31 mattresses were analyzed. In 90% of carpets and mattresses, HDM allergen concentrations were cinically relevant (at least one measurement >0.1 pg Der p 1 + Der f l/g dust). The coefficients ofvariation (CVs) for allergen concentrations in repeated samples over time (55.3-82.0% for the two allergens in beds and carpets) were clearly greater than the CVs for multiple samples collected at the same time (4.0-32.6%). Determination ofallergen mass per square meter of surface instead of concentration per gram of dust resulted in an even greater There are some other considerations of practical relevance that have not been addressed so far. First, the Australian study measured variability in 2-week intervals. This design might fail to detect long-term variations of exposure. Second, the allergen concentration in sampled dust might be massively "diluted" with allergen-free dirt from outside. Is the total sampled mass of allergen a measure of less variability? Finally, can the reliability be improved if sampling is always done by the same trained field worker rather than by residents following written instructions?Answers to these questions should help improve methods of future field studies of HDM allergen exposure. For this purpose it is also useful to estimate how many samples are needed to compensate for the variability caused by influences that cannot easily be controlled by study design. Methods Study DesignVariability ofsampling and dust extraction procedures. To describe the real variability of HDM exposure, it is necessary to assess the impact of laboratory procedures on total variability. Besides the variability of the assay itself (interassay and intra-assay variability; see "Laboratory Analysis") the influence of our sampling and dust extraction procedures on the variability of results was examined in a methodical study.Sample pairs from six mattresses (both longitudinal halves) and six carpets (two squares of 1 m2) were collected at the same time. To evaluate the variability of the extraction procedure, each of these two samples and two additional single samples from two mattresses (n = 26) were divided into two to nine dust portions. These portions were analyzed separately, and the mean and the coefficient of variation (CV) were calculated for each set of portions. The mean was interpreted as the value of the whole original sample. The values of the two original samples collected at one site were then used to calculate CVs for sampling at the same site at the same time.Long-term variability ofallergen concentration at the same site. We retrospectively analyzed dust samples from two field studies in which HDM allergen exposure was determined repeatedly (within 3-24 months).

show abstract

Section: Methods Study Designmentioning

confidence: 99%

Variability of house dust mite allergen exposure in dwellings.

Hirsch¹,

Kuhlisch²,

Soldan³

et al. 1998

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…[5]. Studies by De Lucca et al [106]have shown that when collected by the filtration of inhaled air, the Der p 1 is associated with a range of particles and not just faecal material.…”

Section: Group 1 Allergensmentioning

confidence: 99%

Characterization and Immunobiology of House Dust Mite Allergens

Thomas¹,

Smith²,

Hales³

et al. 2002

Int Arch Allergy Immunol

304

254

View full text Add to dashboard Cite

The examination of house dust mite extracts has indicated that over 30 different proteins can induce IgE antibody in patients allergic to the house dust mite. There are however dominant specificities especially the group 1 and 2 allergens which can account for much of the allergenicity of extracts. Of the 19 denominated allergens, the major IgE binding has been reported for the group 1, 2, 3, 9, 11, 14 and 15 allergens. The high-molecular-weight group 11, 14 and 15 allergens have only recently been described and although high IgE binding has been anticipated from immunoblotting, there is a need for considerable corroboration. Similarly, the study of the group 3 and 9 serine protease allergens has been incomplete. The group 4, 5, 7 and 8 allergens have shown intermediate IgE binding and the group 10 tropomyosins are of interest because of their potential cross-reactivity with allergen from disparate species. Although the progress with the production of recombinant group 1 allergens has been recent, many of the allergens can be produced as high IgE-binding polypeptides. The tertiary structure of the group 2 allergens has been determined from recombinant proteins and they are an excellent model for the investigation of modified allergens. An unexpected property of the group 1, 2 and 3 allergens has been the high degree of polymorphism found by cDNA analysis. It has however been possible to identify sequences to represent the variation in the natural allergens. The group 7 and 14 allergens show secondary modifications which vary in different extracts creating batch variation. While some estimate of the importance of allergens can be obtained from IgE binding, few analyses of T-cell responses have been made and these regulate both the development of, and the protection from sensitization.

show abstract

“…Personal monitoring of allergen entering the airways would considerably improve exposure assessment. Techniques determining nasal HDM allergen exposure as well as the size of the particles on which allergen enters the airways have recently been presented in preliminary reports (20,21).…”

Section: Articles * Variability Of Dust Mite Allergen Exposurementioning

confidence: 99%

Variability of House Dust Mite Allergen Exposure in Dwellings

Hirsch¹,

Kuhlisch²,

Soldan³

et al. 1998

Environmental Health Perspectives

View full text Add to dashboard Cite

Tlhe variability of repeated house dust mite (HDM) allergen determinations at the same site within 3-24 months was evaluated on previously collected samples. Between two and four repeated measurements of Der p 1, a major allergen of Dermatophagoidespteronyssinus and Der f 1, a major allergen of D. farinae, on 46 carpets and 31 mattresses were analyzed. In 90% of carpets and mattresses, HDM allergen concentrations were cinically relevant (at least one measurement >0.1 pg Der p 1 + Der f l/g dust). The coefficients ofvariation (CVs) for allergen concentrations in repeated samples over time (55.3-82.0% for the two allergens in beds and carpets) were clearly greater than the CVs for multiple samples collected at the same time (4.0-32.6%). Determination ofallergen mass per square meter of surface instead of concentration per gram of dust resulted in an even greater CV (72.3-86.7%). The 95% range of expected values was about 10-fold above and below the result of a single determination. We conclude that single determi-nations of HDM allergen in dust give very limited information about long-term exposure of an individual to the allergen. Repeated measurements are recommended. Studies of factors that affect HDM allergen exposure must be planned with appropriate sample sizes. Key words: asthma , house dust mite allergens, indoor allergen eTosure, reliability, repeated determination, sample size, yariability. Environ Health Perspect 106:659-664 (1998). [Online 11 September 1998] hbfp://ehpnetl.niehs.nih.gov/does/998/106p659-664birshlabstract.hbtmI Some studies have shown that exposure to house dust mite (HDM) allergens at home is associated with prevalence of allergic sensitiza-tion (1-3) and asthma (4,5) in susceptible children, but others did not (6,7). The reliability of the determination of indoor exposure to these allergens has not been investigated extensively. Marks and co-workers (8) calculated that threefold above and below a result was the range within which the true value lies with 95% certainty. The authors referred to 117 duplicate determinations of Der p 1, a major allergen of Dermatophagoides pteronyssi-nus, within 2 weeks in Sydney, Australia. In contrast to most other parts of the world (2,3,6,5), Sydney is characterized by extremely high concentrations of Der p 1 [geometric mean 38.9 pg/g dust on mattresses and 22.4 pg/g dust on bedroom floors (8)] and the absence of other HDM allergens. Two recent studies have shown that the prevalence of allergic sensitization in children correlates with exposure to HDM allergen at concentrations far below these levels [0.1 pg Der p 1 + Der f 1 (a major allergen of D. faninae)lg carpet dust (3) or even lower (2]. Thus, it seems reasonable to compare the Australian results with data from a European region with low to moderate levels of Der p 1 and, in addition, moderate concentrations of the HDM allergen Der f 1 to answer two questions: 1) Is the (relative) variability of allergen concentration higher at lower exposure levels? 2) Is the variability of Der f 1 exposure lo...

show abstract

Mite allergen (Der p 1) is not only carried on mite feces

Cited by 38 publications

References 4 publications

Variability of house dust mite allergen exposure in dwellings.

Variability of house dust mite allergen exposure in dwellings.

Characterization and Immunobiology of House Dust Mite Allergens

Variability of House Dust Mite Allergen Exposure in Dwellings

Contact Info

Product

Resources

About