Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL

Abstract

PurposeLacrimal gland benign lymphoepithelial lesion (LGBLEL) is an IgG4-related disease of unknown etiology with a risk for malignant transformation. Estrogen is considered to be related to LGBLEL onset.MethodsSeventy-eight LGBLEL and 13 control clinical samples were collected and studied to determine the relationship between estrogen and its receptors and LGBLEL development.ResultsThe serological analysis revealed no significant differences in the levels of three estrogens be-tween the LGBLEL and control groups. However, immunohistochemical analyses indicated that the expression levels of ERβ and its downstream receptor RERG were relatively lower in LGBLEL samples than in control samples, with higher expression in the lacrimal gland and lower expression in the lymphocyte infiltration region. However, low expression of ERα was detected. The transcriptome sequence analysis revealed upregulated genes associated with LGBLEL enriched in lymphocyte proliferation and activation function; downregulated genes were enriched in epithelial and vascular proliferation functions. The key genes and gene networks were further analyzed. Interactions between B cells and epithelial cells were analyzed due to the identified involvement of leukocyte subsets and epithelial cells. B cell proliferation was found to potentially contribute to lacrimal gland apoptosis.ConclusionTherefore, the tissue-heterogeneous expression pattern of ERβ is potentially related to the clinical manifestations and progression of LGBLEL, although further investigations are required to confirm this finding.

maGENEgerZ: An Efficient Artificial Intelligence-Based Framework Can Extract More Expressed Genes and Biological Insights Underlying Breast Cancer Drug Response Mechanism

“…It has been reported that the NABA MATRISOME ASSOCIATED and NABA SECRETED FACTORS pathways play a key role in breast cancer metastasis through their involvement with extracellular matrix proteins [42,43]. Also, genes linked to the positive regulation of epithelial cell proliferation biological processes are related to the induction of metastasis and inhibition of breast cancer cell apoptosis through the promotion of epithelial cell proliferation via estrogen [44,45]. These results demonstrate the effectiveness of our computational method in unveiling important molecular mechanisms pertaining to breast cancer therapy) that have been approved for breast cancer treatment (see Figure 3) [61][62][63].…”